Cultures of Escherichia coli O157, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Morganella morganii, Salmonella enteritidis, Klebsiella pneumoniae and Listeria monocytogenes, associated to foodborne gastroenteritis outbreaks in the city of Ngaoundéré (Adamawa Region of Cameroon) were provided by the Laboratory of Food Microbiology and Biotechnology of the National School of Agro-industrial Sciences, University of Ngaoundéré (Cameroon). Prior utilization, the strains were sub cultured twice at 37 °C for 24 h in 10 mL of Brain Heart Infusion broth (LiofilChem, Via Scozia, Italy).
Brain heart infusion broth
Manufactured by Liofilchem
Sourced in Italy
Brain Heart Infusion broth is a general-purpose, nutritionally rich microbiological culture medium. It is used to support the growth and cultivation of a wide range of microorganisms, including bacteria, yeasts, and fungi.
Automatically generated - may contain errors
Lab products found in correlation
Sabouraud dextrose agar, by Liofilchem (2 mentions)
Listeria monocytogenes atcc13932, by American Type Culture Collection (1 mentions)
Sabouraud dextrose broth (sdb), by Liofilchem (1 mentions)
Brain heart infusion agar, by Liofilchem (1 mentions)
Mops acid, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
4 protocols using brain heart infusion broth
1
Foodborne Pathogens in Ngaoundéré, Cameroon
2
Pathogenic Bacteria Culture Preparation
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Two reference cultures of pathogenic bacteria, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028 and Listeria monocytogenes ATCC 13932, were obtained from the American Type Culture Collection and used for contamination of model meat samples. Bacterial cultures were stored at the microbiology laboratory of the Food Institute of Kaunas University of Technology at minus 72–74 °C in a VIABANK system. Revitalization was performed in brain heart infusion broth (Liofilchem, Roseto, Italy).
3
Microbial Growth Dynamics in Dental Infections
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E. faecalis ATCC2912 and C. albicans ATCC90028 were cultured for 24 hours at 37°C in Brain-Heart agar (Liofilchem, Italy) and Sabouraud dextrose agar (Liofilchem, Italy), respectively. A cell suspension of 1 × 108 cells/mL was prepared in Brain-Heart infusion broth (Liofilchem, Italy) for each microorganism. Subsequently, the teeth were incubated aerobically at 37°C for 3 days. The inoculum was refreshed after 48 hours.
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E. faecalis ATCC2912 and C. albicans ATCC90028 were cultured for 24 hours at 37°C in Brain-Heart agar (Liofilchem, Italy) and Sabouraud dextrose agar (Liofilchem, Italy), respectively. A cell suspension of 1 × 108 cells/mL was prepared in Brain-Heart infusion broth (Liofilchem, Italy) for each microorganism. Subsequently, the teeth were incubated aerobically at 37°C for 3 days. The inoculum was refreshed after 48 hours.
4
Preparation of Microbial Growth Media
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Mueller-Hinton Broth (MHB), Mueller Hinton Agar (MHA), Brain Heart Infusion Broth (BHIB), Brain Heart Infusion Agar (BHIA), Sabouraud Dextrose Broth and Sabouraud Dextrose Agar from Liofilchem were prepared accordingly to manufacturer instructions. Phosphate buffered saline solution pH 7.4 (PBS) and RPMI 1640 medium supplemented with L-glutamine and buffered with MOPS acid, both from Sigma-Aldrich, were prepared accordingly to manufacturer instructions. Polyethylenimine (branched poly (ethyleneimine) solution, average Mw ~750.000, reference 181978; Sigma-Aldrich) stock solution at 25% (w/v) was used in the experiments.
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