PC cell lines SW1990 and CFPAC-1 and normal pancreatic duct epithelial cell line HPDE were acquired from BeNa Culture Collection (Beijing, China). All cells were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640) medium (Gibco, Carlsbad, CA, USA) added with 10% fetal bovine serum (FBS; Gibco), penicillin (100 units/mL) and streptomycin (100 μg/mL) at 37°C atmosphere with 5% CO2.
Sw1990
Manufactured by BNCC
Sourced in China
The SW1990 is a laboratory equipment designed for general scientific applications. It features a compact and durable construction, with precise controls and reliable performance. The core function of the SW1990 is to provide a controlled environment for various laboratory experiments and procedures.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Cfpac 1, by BNCC (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Panc 1, by BNCC (2 mentions)
Hs766t, by BNCC (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Gemcitabine, by Selleck Chemicals (1 mentions)
Aspc 1, by BNCC (1 mentions)
Hpde6 c7, by BNCC (1 mentions)
Bxpc 3, by BNCC (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Iscove modified dulbecco medium, by Merck Group (1 mentions)
Cfpac 1, by Merck Group (1 mentions)
Sw1990, by Merck Group (1 mentions)
Leibovitz l 15 medium, by Merck Group (1 mentions)
M3 base f culture medium, by INCELL (1 mentions)
Penicillin, by Solarbio (1 mentions)
Streptomycin, by Solarbio (1 mentions)
4 protocols using sw1990
1
Cell Culture of Pancreatic Cell Lines
2
Gemcitabine Cytotoxicity in PC Cell Lines
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Four human PC cell lines (SW1990, AsPC-1, PANC-1 and BxPC-3) and normal human pancreatic ductal epithelial cell line HPDE6c7 were bought from BeNa Culture collection (Beijing, China) and cultured in RPMI 1640 medium (Gibco, Grand Island, NY, U.S.A.). Culture medium contained 10% fetal bovine serum (Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin–streptomycin (Invitrogen). Cells were allowed to grow in a humidified incubator at 37°C with 5% CO2.
SW1990 and BxPC-3 cell lines (3 × 104 cells/well) were planted in 96-well plates and treated with various concentrations (ranging from 0 to 128 μg/ml) of gemcitabine (Selleck, Houston, TX, U.S.A.) for 72 h.
SW1990 and BxPC-3 cell lines (3 × 104 cells/well) were planted in 96-well plates and treated with various concentrations (ranging from 0 to 128 μg/ml) of gemcitabine (Selleck, Houston, TX, U.S.A.) for 72 h.
3
Culturing Human Pancreatic Cell Lines
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Human pancreatic ductal cell line (HPNE) and PC cell lines, including PANC-1, SW1990,
HS766T, and CFPAC-1, were purchased from BeNa Culture Collection (Beijing, China) and
cultured in incubator with 100% humidity and 5% CO2 at 37°C. The HPNE cells
were grown in Dulbecco modified Eagle medium (DMEM; Sigma, St Louis, Missouri) containing
1 volume of M3 Base F culture medium (InCell Corp, San Antonio, Texas), 3 volumes of
glucose-free DMEM, 10% fetal bovine serum (FBS; (Invitrogen, Carlsbad, California), 5.5 mM
glucose, 10 ng/mL epidermal growth factor (EGF), and 50 µg/mL gentamycin. The DMEM medium
with 10% FBS was applied to culture PANC-1 and HS766T cells. SW1990 cells were maintained
in Leibovitz L-15 Medium (Sigma). Furthermore, CFPAC-1 cells were cultured in Iscove
Modified Dulbecco Medium (Sigma) adding 10% FBS.
HS766T, and CFPAC-1, were purchased from BeNa Culture Collection (Beijing, China) and
cultured in incubator with 100% humidity and 5% CO2 at 37°C. The HPNE cells
were grown in Dulbecco modified Eagle medium (DMEM; Sigma, St Louis, Missouri) containing
1 volume of M3 Base F culture medium (InCell Corp, San Antonio, Texas), 3 volumes of
glucose-free DMEM, 10% fetal bovine serum (FBS; (Invitrogen, Carlsbad, California), 5.5 mM
glucose, 10 ng/mL epidermal growth factor (EGF), and 50 µg/mL gentamycin. The DMEM medium
with 10% FBS was applied to culture PANC-1 and HS766T cells. SW1990 cells were maintained
in Leibovitz L-15 Medium (Sigma). Furthermore, CFPAC-1 cells were cultured in Iscove
Modified Dulbecco Medium (Sigma) adding 10% FBS.
4
Cell Culture of Pancreatic Lines
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Normal human pancreatic duct epithelial cell line (HPDE) and two pancreatic cancer cell lines (Hs 766T and SW1990) were purchased from BeNa Culture Collection (Beijing, People's Republic of China) and cultured with Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) added with 10% fetal bovine serum (FBS; Gibco) and 1% antibiotic (100 units/mL penicillin/100 μg/mL streptomycin; Solarbio, Beijing, People's Republic of China) in a 37°C incubator with 5% CO2/95% air.
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