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Fluorescein isothiocyanate (fitc)

Manufactured by Dingguo
Sourced in China

Fluorescein isothiocyanate (FITC) is a fluorescent dye commonly used in various laboratory applications. It is a derivative of fluorescein, which exhibits green fluorescence when excited by light. FITC can be used to label and track biomolecules, such as proteins, antibodies, and nucleic acids, in order to study their behavior and localization within a biological system.

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4 protocols using fluorescein isothiocyanate (fitc)

1

Docetaxel-Loaded Albumin Nanoparticles

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Docetaxel (DTX) was purchased from Guilin Huiang Biopharmaceutical Co., Ltd. (Guilin, China). HSA (20%) was purchased from Octapharma (Vienna, Austria). Dimethylsulfoxide (DMSO), 3-[4,5-dimethylthiazol-2-yl]-2,5,-diphenyltetrazolium bromide (MTT), and 4′,6-diamidino-2-phenylindole (DAPI) were supplied by Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Fluorescein isothiocyanate (FITC) was obtained from Beijing Dingguo Bio-Technology Co., Ltd. (Beijing, China). Human lung cancer cell line A549 was acquired from American Type Culture Collection (ATCC, Logan, UT, USA). Organic solvents of chromatography grade were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd. (Beijing, China).
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2

Synthesis and Characterization of Peptide-Based Compounds

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Boc-Arg (Pbf)-OH and Boc-Lys (Boc)-OH were purchased from GL Biochem Ltd. (Shanghai, China). CPT was purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). Trifluoroacetic acid (TFA) was obtained from Xiya Reagent Co., Ltd. (Linyi, China). EDC hydrochloride was provided by J&K Scientific Ltd. (Beijing, China). N-Hydroxysuccinimide and DMAP were supplied by Aladdin Co. Ltd. (Shanghai, China). 4’-6-diamidino-2-phenylindole was purchased from Sigma-Aldrich (St. Louis, MO, USA). Methyl thiazolyl tetrazolium (MTT) was obtained from Amresco (Solon, Ohio, USA). Annexin V-FITC apoptosis detection kit, ROS Assay Kit (DCFH-DA), Mitochondrial Membrane Potential Assay Kit with Rhodamine 123, Fluo-4 AM, and MitoTracker Red were obtained from Beyotime (Shanghai, China). Fluorescein isothiocyanate (FITC) was obtained from Dingguo Biotechnology Company (Beijing, China).
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3

Synthesis and Characterization of Polymeric Nanoparticles

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PTX and DOX were provided by Xi’an Natural Field Bio-Technique Co., Ltd. (Xi’an, China) and Hubei Yuancheng Company (Hubei, China) with a purity >99%. Styrene (St) monomer, potassium persulfate (K2S2O8), anhydrous ethanol, tetrahydrofurane, and calcium chloride (CaCl2) were purchased from Tianjin Yongsheng Fine Chemical Co., Ltd. (Tianjin, China). Ammonium dihydrogen phosphate (NH4H2PO4) and dichloromethane were supplied by Tianjin Guangfu Fine Chemical Co., Ltd. (Tianjin, China). Soya lecithin was purchased from Shanghai Tywei Pharmaceutical Co., Ltd. (Shanghai, China), and cholesterol was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Fluorescein isothiocyanate (FITC) and rhodamine phalloidin were purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd. (Beijing, China) and trypsin was purchased from Genview (Beijing, China). Deionized water was used in all experiments.
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4

Fenofibrate-loaded Sustained-release Nanoparticles

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Fenofibrate was provided by the Dalian Meilun Company (Dalian, China) with a purity >99%. Methyl methacrylate (MMA) and gelatin were obtained from Sinopharm Chemical Reagent Company, Ltd. (Shanghai, China). Potassium persulfate, tetrahydrofurane, acetic acid, and anhydrous ethanol were purchased from Tianjin Yongsheng Fine Chemical Company, Ltd. (Tianjin, China). Fluorescein isothiocyanate (FITC) and Rhodamine B were purchased from Beijing Dingguo Changsheng Biotechnology Company, Ltd. (Beijing, China). Trypsin was provided by GENView (Beijing, China). Dichloromethane was obtained from Tianjin Guangfu Fine Chemical Company, Ltd. (Tianjin, China). Commercial sustainedrelease capsules were produced by Ethypharm Pharmaceutical Company, Ltd. (Shanghai, China) and deionized water was used in all experiments.
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