After a 3-hour fast, whole blood was collected via tail snip and allowed to clot on ice for 30 minutes. Samples were then centrifuged at 3000g for 15 minutes. The resultant serum supernatant was used for analysis. Serum insulin and IGF-1 levels were measured with ELISAs from ALPCO (Salem, NH). Serum insulin levels were measured alongside glucose at 6, 10, 16, 22, and 29 weeks of age and IGF-1 levels were determined at sacrifice.
Elisas
Manufactured by ALPCO
Sourced in United States
ELISAs are laboratory assays used to detect and quantify specific proteins, hormones, antibodies, or other analytes in biological samples. These assays utilize antibodies to capture and detect the target analyte, providing a quantitative measurement of its concentration.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using elisas
1
Serum Insulin and IGF-1 Evaluation
2
Biomarkers of Inflammation and CVD Risk
3
Serum Hormone Levels Before and After Supplementation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting blood samples were aseptically collected into 5 mL serum separator tubes (BD Vacutainer, Franklin Lakes, NJ, USA) from the antecubital vein before and after 12 weeks of supplementation and training during the same time of day (±2 hours). Notably, SPC, WPC or PLA supplementation occurred up until the day prior to blood and tissue extraction. After collection in serum tubes, blood was centrifuged at 4,000 g at 4 °C for 5 min. Serum was then pipetted into cryotubes and placed in a −80 °C freezer for batch-processing. 17β estradiol and total testosterone concentrations in serum were measured using commercially available enzyme linked immunosorbent assay kits (ELISAs, ALPCO Diagnostics, Salem, NH, USA).
4
Biomarkers in Gestational Diabetes Mellitus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the two blood collections prior to the GDM screening test (i.e., at enrollment visit and visit 1), biomarkers were measured among all the case (n = 107) and control subjects (n = 214). At the following blood collections at visits 2 and 4, biomarkers were measured among all the case subjects (n = 107) and one of their control subjects (n = 107). Plasma concentrations of total IGF-I, IGFBP-2, and IGFBP-3 were measured in ng/mL using ELISAs (ALPCO Diagnostics, Salem, NH). Plasma concentrations of glucose, insulin, and C-reactive protein (CRP) were measured using hexokinase, immunosorbent, and immunoturbidimetric assays (Roche Diagnostics, Indianapolis, IN), respectively. Plasma adiponectin levels were measured using a quantitative sandwich enzyme immunoassay (Beckman Coulter, Inc., Fullerton, CA). Plasma lipids (total cholesterol, LDL cholesterol, HDL cholesterol, and triglycerides) were measured using enzymatic assays (Roche Diagnostics, Indianapolis, IN). All the inter- and intra-assay coefficients of variation were <6.7%. All assays were performed without knowledge of GDM status.
5
Plasma Biomarker Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma samples were obtained from venous blood collected on potassium‐EDTA coated tubes (BD Vacutainers) and centrifuged according to the manufacturer's protocol. The samples were immediately aliquoted on ice and frozen at −80°C until analysis. All biomarker measurements were made without knowledge of genotypes. Lipid concentrations in serum were assessed by enzymatic/colorimetric assay using the Dimension Vista laboratory system (Siemens). Apolipoprotein A‐I and apolipoprotein B concentrations were obtained by nephelometry on a BN ProSpec nephelometer (Siemens). The plasma concentrations of lecithin/cholesterol acyltransferase and myeloperoxidase were measured by ELISAs from Alpco Diagnostics. Nuclear magnetic resonance profiling of HDL subclasses was performed by Liposcience (Raleigh, NC).
6
Quantifying Adipokine Secretion in ASCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Conditioned media from induced ASCs and 3T3-L1 wells, as well as non-induced controls were collected at 14-days post-induction, where the media had been conditioned for 48 hours prior to analysis. Leptin and adiponectin content in the media was measured by ELISA following the manufacturer’s protocols (Crystal Chem Inc., IL, USA). Total leptin and adiponectin levels in the supernatants were normalized to total intracellular protein content measured using the Bio-Rad Protein Assay. Additionally, for the in vivo studies, ELISAs (ALPCO, NH, USA) were performed following the manufacturer’s protocols for insulin and adiponectin, while cholesterol was assessed by CHOD-PAP kit (Roche Diagnostics, Indianapolis, IN) and triglyceride analysis was conducted by Triglycerol/Glycerol kit (Roche Diagnostics, Indianapolis, IN) following manufacturer’s protocols. Analyses for the in vivo studies were conducted using serum collected from congenic mice fed on a 5-week HFD.
7
Glucose Tolerance Test in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Mice were fasted 4 hours prior to testing. Fasted blood glucose was measured via a glucometer (OneTouch Ultra). Glucose tolerance testing was conducted by administration of 1 g/kg of glucose by intraperitoneal injection, and blood glucose was monitored at 0, 15, 30, 60, and 120 minutes via tail vein puncture. Glucose area under the curve (AUC) was calculated. At sacrifice, blood was collected, and plasma was isolated. ELISAs (ALPCO, NH, USA) were performed following the manufacturer's protocol for insulin, cholesterol was assessed by CHOD-PAP kit (Roche Diagnostics, Indianapolis, IN), and triglyceride analysis was conducted by Triglycerol/Glycerol kit (Roche Diagnostics, Indianapolis, IN) following manufacturer's protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!