Citric acid monohydrate, sucrose, ethylenediamine, protoporphyrin IX, sodium chloride, (N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide), N-Hydroxysuccinimide, 2-hydroxyethylagarose, formaldehyde, perinaphthenone, 2-(N-Morpholino) ethanesulfonic acid, acetone, dimethyl sulfoxide, 2-mercaptoethanol and N,N-dimethylformamide were acquired from Sigma Aldrich (United Kingdom). Dulbecco’s modified Eagle’s medium (DMEM, high glucose), Dulbecco’s modified Eagle’s medium (DMEM, high glucose, without phenol red), foetal bovine serum (FBS), Quant-iT Picogreen dsDNA quantification kit, Pierce LDH cytotoxicity assay kit, and trypsin-EDTA were obtained from Thermo Fisher (United Kingdom). Syringe filters with a 0.2 μm pore size were acquired from Sarstedt (United Kingdom). 1 KDa MWCO, 6.4 ml/cm dialysis tubing was acquired from Spectrum Labs (United States of America). All chemicals were used as received unless stated otherwise. Deionized water was used for all buffers and samples in experiments. Septa steel ring caps and 35 ml glass reaction vessels were obtained from CEM Corporation (United Kingdom).
Perinaphthenone
Manufactured by Merck Group
Sourced in United Kingdom, United States
Perinaphthenone is a chemical compound used in laboratory settings. It serves as a reagent for various analytical and synthetic applications. The core function of Perinaphthenone is to facilitate chemical reactions and analyses, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.
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Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Furfuryl alcohol ffa, by Merck Group (2 mentions)
Protoporphyrin 9, by Merck Group (1 mentions)
Quant it picogreen dsdna quantification kit, by Thermo Fisher Scientific (1 mentions)
Syringe filter, by Sarstedt (1 mentions)
Pierce ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions)
2 hydroxyethylagarose, by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide, by Merck Group (1 mentions)
2 n morpholino ethanesulfonic acid, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Citric acid monohydrate, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Ethylenediamine, by Merck Group (1 mentions)
Septa steel ring caps, by CEM Corporation (1 mentions)
35 ml glass reaction vessels, by CEM Corporation (1 mentions)
7 protocols using perinaphthenone
1
Synthesis and Characterization of Fluorescent Conjugates
2
Synthesis of Heterocyclic Compounds
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Perinaphthenone (Sigma-Aldrich), new methylene blue (NMB, Sigma-Aldrich), anthracene (Sigma-Aldrich), naphthalene (Sigma-Aldrich), 1-methyl-2-pyrrolidone, (Sigma-Aldrich), 2-methylbenzo[1,2-d]oxazole (Sigma-Aldrich), 2-methylnaphtho[1,2-d]oxazole (AK Scientific, Inc.), 2-methylnaphtho[3,2-d]oxazole (TCI), 2-aminophenol (Sigma-Aldrich), 1-amino-2-naphthol hydrochloride (Sigma-Aldrich), 2-amino-3-naphthol (Sigma-Aldrich), furfural (Sigma-Aldrich), 5-methylfurfural, (Sigma-Aldrich), 5-bromofurfural (Sigma-Aldrich), 5-phenylfurfural (Sigma-Aldrich) and furoyl chloride (Sigma-Aldrich) were used as received. All solvents used were UV or HPLC grade.
3
Protease Activity Assay Protocol
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Materials. Aeromonas proteolytica aminopeptidase (BLAP, PDB 1RTQ, Sigma, 116.51 units mg -1 ), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA, BioUltra, 99.5-101.5 %), iodoacetamide (IAM, 99 %), DL-dithio-threitol (DTT, 99 %), perinaphthenone (PN, 97 %), 7-hydroxy-4-methylcoumarin, 7-leucine-7-amido-4-methylcoumarin hydrochloride, were purchased from Sigma. Furfuryl alcohol (FFA, Merck, 98%) was distilled prior to use.
ProteaseMAX TM (Promega, lyophilized, V207A), sequencing grade chymotrypsin (Promega, lyophilized, V106A), iRT peptides (iRT Kit, Biognosys) were used for protein digestion and analytical measurements, respectively. Further chemicals purchased from commercial vendors are listed in Section S1.
ProteaseMAX TM (Promega, lyophilized, V207A), sequencing grade chymotrypsin (Promega, lyophilized, V106A), iRT peptides (iRT Kit, Biognosys) were used for protein digestion and analytical measurements, respectively. Further chemicals purchased from commercial vendors are listed in Section S1.
4
Synthesis and Characterization of Thiophene-C60 Dyads
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Thiophene– and selenophene–C60 dyads (Figure 1 ) were synthesized based on previously reported procedures [30 (link),31 (link),32 (link),33 (link)]. The detailed synthetic route and the identification of the obtained products are shown in the Supporting Information . 2,2′:5′,2″-Terthiophene (TTh) and fullerene C60 were purchased from Alfa Aesar, Haverhill, MA, USA (purity 99%) and Sigma Aldrich, St. Louis, MO, USA (purity 98%), respectively. dichloromethane (HPLC grade, Sigma Aldrich, St. Louis, MO, USA) was used as a solvent for UV–Vis spectroscopy and photophysical studies. Tetraphenylcyclopentadienone (TPCPD) (Acros Organics, Geel, Belgium, purity 99%) in dichloromethane was used as a specific 1O2 quencher. Perinaphthenone (Sigma Aldrich, St. Louis, MO, USA, purity 97%) was applied as a reference for the determination of the quantum yield of singlet oxygen production. Photooxidation of 1,5-dihydroxynaphtalene (DHN, Sigma Aldrich, St. Louis, MO, USA, purity 97%) was conducted in dichloromethane: methanol (Across Organics, Geel, Belgium, purity 99.9%) mixture (9:1 v/v).
5
Synthesis and Purification of Perinaphthenone
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Perinaphthenone (PN), sodium bromide, guanidinium chloride, lithium chloride and sodium phosphate dibasic were purchased from Sigma Aldrich. Furfuryl alcohol (FFA) was obtained from Merck, and puried by distillation prior to use. Sodium bicarbonate, sodium chloride and magnesium dichloride hexahydrate were also purchased from Merck. Potassium dihydrogen phosphate, magnesium sulfate heptahydrate, calcium dichloride dehydrate, sodium perchlorate monohydrate and potassium chloride were from Fluka. D 2 O was obtained from Armar. All solvents used for the analysis were of HPLC grade. All aqueous solutions were prepared in ultrapure water (resistivity > 18 MU, Barnstead Nanopure Diamond System). N 2 (99.999%) and O 2 (99.9995%) were purchased from CarbaGas.
6
Antioxidant Enzyme Kinetics Study
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Prednisolone 99% (Predn), superoxide dismutase from bovine erythrocytes Biuret>95% (SOD) and catalase from bovine liver (CAT) Biuret> 95%. Sodium azide99% (NaN 3 ) and isopropanol 99.7% (isoOH) were purchased from Merck. Furfuryl acetate 99% (FFAc), Perinaphthenone 97% (PN) and Rose Bengal 95%were provided by Aldrich Chemical Company. Acetonitrile (MeCN), HPLC quality, were provided by Sintorgan.
All these compounds were used as received. The water used was triply distilled. All measurements were carried out with freshly prepared solutions at 298 K, except when other temperature is indicated. In order to increase the solubility of Predn, 1% MeCN was added.
Where otherwise indicated, "aqueous solution" refers to water/MeCN 99/1% v/v mixture.
All these compounds were used as received. The water used was triply distilled. All measurements were carried out with freshly prepared solutions at 298 K, except when other temperature is indicated. In order to increase the solubility of Predn, 1% MeCN was added.
Where otherwise indicated, "aqueous solution" refers to water/MeCN 99/1% v/v mixture.
7
Synthesis and Characterization of Gold(I) Complexes
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All manipulations were performed under prepurified N 2 using standard Schlenk techniques. All solvents were distilled from appropriate drying agents. 4-Ethynylamine (Aldrich, 97%), perinaphthenone (Aldrich, 97%) and alginic acid sodium salt (Aldrich) was used as received. Literature methods were used to prepare [AuCl(tht)], 43
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