A panel of ten BTC cell lines was used in this study: EGI-1 (ACC 385 [32 (link)]) and TFK-1 (ACC 344 [33 (link)]) cells were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ; https://www.dsmz.de , last accessed on 1 August 2021); HuCCT1 (JCRB0425 [34 (link)]), HuH28 (JCRB0426 [35 (link)]), KKU-055 (JCRB1551), KKU-100 (JCRB1568 [36 (link)]), KKU-213 (JCRB1557 [37 (link)]), NOZ (JCRB1033 [38 ]), OCUG-1 (JCRB0191 [39 (link)]) and OZ (JCRB1032 [40 (link)]) were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB; https://cellbank.nibiohn.go.jp , last accessed on 1 August 2021). The immortalized human cholangiocyte cell line MMNK-1 (JCRB1554 [41 (link)]) was purchased from JCRB. All cell lines were cultured in L-glutamine-free Dulbecco’s Modified Eagle Medium (DMEM) containing 4.5 g/L glucose (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Medium was supplemented with 10% fetal bovine serum (FBS; Serana Europe GmbH, Pessin, Germany) and 1% penicillin/streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Cells were cultured at standard conditions (37 °C, 21% O2, 5% CO2, and 98% humidity).
Egi 1 acc 385
Manufactured by Leibniz Institute DSMZ
The EGI-1 (ACC 385) is a laboratory equipment product offered by the Leibniz Institute DSMZ. It is a device designed for the cultivation and analysis of microorganisms. The core function of the EGI-1 is to provide a controlled environment for the growth and study of various microbial species.
Automatically generated - may contain errors
Lab products found in correlation
Ocug 1, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Huh28, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Kku 055, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Kku 100, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Kku 213, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Hucct1, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Tfk 1, by Leibniz Institute DSMZ (1 mentions)
Mmnk 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
2 protocols using egi 1 acc 385
1
Establishment and Characterization of Biliary Tract Cancer Cell Lines
2
Establishment and Culture of BTC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human BTC cell lines HuCC-T1 (JCRB0425 [29 ]), HuH-28 (JCRB0426 [30 ]), OCUG-1 (JCRB0191 [31 (link)]), OZ (JCRB1032 [32 (link)]), NOZ (JCRB1033 [33 (link)]), KKU-055 (JCRB1551), KKU-100 (JCRB1568 [34 (link)]), KKU-213 (JCRB1557), and immortalized human cholangiocyte cell line MMNK-1 (JCRB1554 [35 (link)]) were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB). Human BTC cell lines EGI-1 (ACC385) and TFK-1 (ACC344 [36 (link)]) were purchased from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ). All cell lines were cultured in L-glutamine-free Dulbecco’s Modified Eagle Medium (DMEM) containing 4.5 g/l glucose (Catalogue #: 11,960,044, Gibco, Thermo Fisher Scientific, Vienna, Austria). Medium was supplemented with 10% foetal bovine serum (FBS; Catalogue #: S-FBS-SA-015, Serana Europe GmbH, Pessin, Germany) and 1% Penicillin-Streptomycin (Catalogue #: P4333, Sigma-Aldrich Handels GmbH, Vienna, Austria). Cells were cultured under standard conditions (37°C, 21% O2, 5% CO2, and 98% humidity).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!