Trizol reagent and reverse transcription kit

A fourth-generation poly (amidoamine) dendrimer (G4.0 PAMAM)) was obtained from Chenyuan Molecular (Weihai, Shandong, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinimidyl (polyethylene glycol)-2000] (DSPE-PEG₂₀₀₀-NHS) was obtained from Ponsure Biotechnology (Shanghai, China). The Cell Counting Kit 8 (CCK8), and Lyso-Tracker Green were supplied by Beyotime Biotechnology (Shanghai, China). The Alizarin Red Staining Kit was bought from Solarbio Science & Technology Co. (Beijing, China). Fluorescein isothiocyanate (FITC), Nile Red, Rhodamine B (Rho B), ALA, dimethyl sulfoxide (DMSO), and Streptozotocin (STZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The collagen membrane was obtained from Geistlich Pharma AG (Wolhusen, Switzerland). AGE glycated bovine serum albumin (AGE-BSA) was bought from Abcam (Cambridge, MA, USA), Mino was purchased from AbMole Bioscience (Houston, TX, USA). Primary antibody for inducible nitrous oxide synthase (iNOS) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The TRIzol reagent and reverse transcription kit were bought from Invitrogen (Carlsbad, CA, USA) and Takara Shuzo (Kyoto, Japan). All other reagents and products for cell culture were bought from GIBCO (Gaithersburg, MD, USA) unless otherwise stated.