Formalin-fixed, paraffin-embedded sections (3 μm thick) were stained with H&E (MilliporeSigma) or periodic acid-Schiff (MilliporeSigma). Slices were scored by a nephropathologist in a blinded manner. A kidney injury score grading scale from 0 to 5 was used to assess the severity of the injury as follow: 0 = no lesions; 1 = minimal injury characterized by the occurrence of necrosis and debris; 2 = mild injury with single-cell necrosis, pyknotic cells, and apoptosis; 3 = moderate injury characterized by tubular distension, vacuolation, and some cellular debris; 4 = severe injury with occasional hyaline casts observed, patchy epithelial necrosis in all segments, and loss of epithelial lining; and 5 = very severe injury characterized by extensive tubular epithelial necrosis in all segments, loss of the epithelial layer from many tubules, widespread intraluminal cellular debris, and frequent hyaline casts particularly prominent in the medullary region (72 (link)).
Periodic acid schiff
Manufactured by Merck Group
Sourced in United States, United Kingdom, Japan, Germany
Periodic Acid-Schiff (PAS) is a histochemical staining technique used in laboratory settings. It is designed to detect the presence of certain carbohydrates, particularly glycogen and mucopolysaccharides, in biological samples. The PAS reaction involves the oxidation of these carbohydrates by periodic acid, followed by the subsequent reaction with Schiff's reagent, which results in the formation of a characteristic purple-magenta color.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Merck Group (4 mentions)
Alcian blue, by Merck Group (3 mentions)
Hematoxylin and eosin h e, by Merck Group (3 mentions)
Axioskop 2, by Merck Group (3 mentions)
Optimal cutting temperature compound, by Thermo Fisher Scientific (3 mentions)
Axioskop 2 microscope, by Zeiss (3 mentions)
Xylene, by Avantor (3 mentions)
Leica dmr upright microscope, by Leica Microsystems (3 mentions)
Mitotracker red, by Thermo Fisher Scientific (3 mentions)
Schiff s reagent, by Merck Group (2 mentions)
Nis elements software, by Nikon (2 mentions)
Tryphostin ag1478, by Merck Group (2 mentions)
Image pro plus 7, by Media Cybernetics (2 mentions)
Toluidine blue, by Merck Group (2 mentions)
Paraformaldehyde (pfa), by Merck Group (2 mentions)
Formalin, by Merck Group (2 mentions)
Giemsa, by Merck Group (2 mentions)
Formaldehyde, by Merck Group (2 mentions)
Hematoxylin eosin he, by Merck Group (2 mentions)
Masson s trichrome, by Merck Group (2 mentions)
74 protocols using periodic acid schiff
1
Kidney Injury Scoring Protocol
2
Detecting Mucin-Secreting Goblet Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To detect mucin-secreting goblet cells, AB-PAS and mucicarmine staining were performed. Alcian blue (AB, Abcam), periodic acid-Schiff (PAS; MilliporeSigma, Burlington, MA, USA), and mucicarmine (Abcam) stain kits were applied to sectioned tissue slides following the manufacturers' recommended protocols.
3
Histological Analysis of HH35 Hearts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sections from HH35 hearts were prepared as described for histological studies and then subjected to diastase (Sigma), periodic acid‐Schiff (Merck) and Haemalum (Haematoxylin; Sigma) as per manufacturers’ instructions. Quantitative analysis was performed in imagej using colour deconvoluted images (Ruifork & Johnston 2001 ). Images were taken using an AxioPlan (Zeiss). Statistics were performed using a two‐way analysis of variance (anova ).
4
Histological Staining of Paraffin Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded sections were prepared as described above. periodic acid Schiff staining was performed with periodic acid (0.5%) (Merck), Schiff’s reagent (Merck) and hematoxylin (Fluka). For Sirius red staining, sections were treated with 0.2% phosphomolybdic acid, 0.1% Sirius red in 3% picric acid, 0.01 mol/L HCl, 70% and 100% ethanol in the order specified.
5
Histological Analysis of Intestinal Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fixated tissues were dehydrated in an ethanol gradient of 77%‐99% (absolute ethanol, cat. no. 83813.360, VWR), cleared using xylene (cat. no. 28973.363, VWR) and embedded in paraffin (cat. no. 2270.60.60, Hounisen, Skanderborg, Denmark). Sections of 2 μm were stained with Meyer's Hematoxylin (cat. no. AMPQ00254.0500, Ampliqon, Odense, Denmark) and Eosin Y (cat. no. 341973R, VWR) to identify eosinophils and 0.5% Toluidine Blue (TB; cat. no. 89640, Sigma‐Aldrich) in 1 M hydrochloric acid to identify mast cells, or Periodic acid‐Schiff (PAS; periodic acid: Cat. no. 1.00524.0025, Merck and Schiff′s reagent: Cat. 3952016, Sigma‐Aldrich) to identify goblet cells.34 Slides were examined using a Leica DMR upright microscope (Leica Microsystems GmbH, Wetzlar, Germany). The software ImagePro Plus 7.0 (MediaCybernetics, Rockville, MD, US) was used for image analysis. Villus length was measured from the villus tip to the crypt‐villus junction. Cell count and villi length in the SI were averaged from three sections of three similar consecutive villi and crypts. Cell count in the colon was averaged from six individual crypts. Analysis of histological sections was performed blinded.
6
Lubricin and Cartilage Glycoprotein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified native lubricin and SF samples from CG incubations, and also SF samples (16 OA patient samples, 2 μl SF) used for screening of endogenous CG or lubricin degradation fragments, were reduced with 50 mM dithiothreitol (DTT) (Merck KGaA) followed by boiling at 95 °C for 15 minutes, and alkylation by 125 mM iodoacetamide (Sigma-Aldrich, St. Louis, MO, US) in dark for 45 minutes. Samples were analysed on NuPAGE Tris-acetate 3–8% gel (Invitrogen, Thermo Fisher Scientific, Waltham, MA, US). One μg of rhPRG4 or 22 ng CG (for endogenous CG assay) were included on each gel as controls. Molecular weight was compared to PageRuler Plus Prestained Protein Ladder (10 to 250 kDa, Thermo Fisher Scientific, USA). Gels were stained either with Coomassie brilliant blue R-250 (Bio-Rad Laboratories, Hercules, CA, US) or with Periodic Acid-Schiff (PAS) (Sigma-Aldrich) according to the manufacturers’ instructions. Lubricin band intensities were plotted and peak values were calculated by ImageJ (ImageJ 1.50i, USA)53 (link).
7
Murine Mastitis Induction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6 lactating female mice (6–8 wk old; 10–14 d after parturition) were housed in specific pathogen-free facilities at the University of Calgary with ad libitum access to food and water. Mice were inoculated intramammarily with either P. zopfii GT-II (50 µL containing 1 × 105 CFU/mL) or an equal volume of phosphate buffered saline (PBS) (control) in the left fourth and right fourth (L4 and R4) mammary glands. Mice were euthanized 4 d post inoculation (dpi) to collect mammary tissue samples. Tissues were mixed into TRIzol (Invitrogen, Carlsbad, CA, USA) and later homogenized for quantitative PCR (qPCR) or fixed in 10% formalin solution, embedded in paraffin wax, sectioned with a microtome (5 µm) and stained with hematoxylin and eosin (H&E; Sigma, USA) for histological examination25 (link) and with Periodic Acid-Schiff (PAS; Sigma, USA) and Grocott-Gomori’s methenamine silver stain (GMS) as a screen for fungal organisms.
8
Histological Quantification of Ocular Goblet Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eyeballs and adnexa were surgically excised and fixed with 4% paraformaldehyde (Biosesang, Seongnam-si, Gyeonggi-do, South Korea) for 3 days at 4°C followed by a 3 day PBS (Welgene, Gyeongsan-si, Gyeongsangbuk-do, South Korea) rinse, cryoprotected using a sucrose gradient and embedded in OCT compound (Tissue-Tek; Sakura Finetek, Torrance, CA, USA) at -80°C. A 7 μm section was cut and dried at room temperature. The sectioned tissue was stained with periodic acid-Schiff (PAS; Sigma-Aldrich) for 15 min, with haematoxylin counterstaining (Muto Pure Chemicals, Tokyo, Japan) for 5 min. Each slide was examined and photographed under a microscope (Ti-U; Nikon Instruments), and images were captured using NIS-Elements software (Nikon Instruments). PAS-positive goblet cells were counted with ImageJ on three individual slides for each treatment by a blinded investigator (C.Y.Y.).
9
Quantifying Renal Tubular Necrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum levels of creatinine were determined by using the quantitative enzymatic method of the Creatinine LiquiColor Test kit (Stanbio Laboratory, Texas) according to the manufacturer's protocol, while blood urea nitrogen (BUN) was measured by using BUN Assay kit (Jiancheng bio, Nanjing, China). Kidney tissues were fixed with Histochoice Tissue Fixation MB (AMRESCO, VWR Life Science, PA) and tubular necrosis was examined in paraffin sections (3 µm) by hematoxylin and Eosin (HE) or Periodic Acid-Schiff (PAS) staining (Sigma, St. Louis, MO). A total of 500 cortical tubules were examined and the numbers of tubules with necrosis was counted and expressed as percentage as previously described 8 (link)-11 (link).
10
Stereological Analysis of Liver Steatosis and Gut Goblet Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver and cecum fragments fixed in Millonig-buffered formalin (pH 7.2-7.4) were subsequently dehydrated, diaphanized, embedded in Paraplast Plus (Sigma–Aldrich, St. Louis, MO, United States) and sectioned (5 μm thick) with a microtome. Slides stained with hematoxylin and eosin (liver) or Alcian Blue (Sigma Chemical Company-pH 2.5) plus Periodic Acid-Schiff (PAS, intestine-Sigma Chemical Company) were photographed using a Leica DMRBE microscope (Wetzlar, Germany) and an Infinity Lumenera digital camera (Ottawa, ON, Canada). The images were analyzed in a blinded manner with STEPanizer (www.stepanizer.com ) as described below:
Hepatic stereology: Five animals per group and ten images per animal were analyzed. The volume density of liver steatosis [Vv (liver, st)] was estimated by the point-counting technique, following the formula: Vv (liver, st) = Pp (liver, st)/PT (Pp is the number of points that reached fat droplets, and PT is the total test points). The images were analyzed with STEPanizer using a 36-point test system[17 (link)].
Gut stereology: The number of goblet cells per area [QA (goblet)] was estimated using STEPanizer. All goblet cells within the test area were counted, except those touching the forbidden lines. The result was divided by the test area measured in mm2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!