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Goat anti rat af594

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Goat anti-rat AF594 is a secondary antibody conjugate designed for use in immunofluorescence applications. It is generated by conjugating goat anti-rat IgG antibodies with the Alexa Fluor 594 fluorescent dye. This product is intended for detection and visualization of rat target proteins in various biological samples.

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Lab products found in correlation

Prolong gold antifade, by Thermo Fisher Scientific (3 mentions) Goat serum, by Vector Laboratories (2 mentions) Clone cc1, by Abcam (2 mentions) Clone soia15, by Thermo Fisher Scientific (2 mentions) Dm5500 epifluorescence microscope, by Leica (2 mentions) Mouse on mouse immunodetection kit, by Vector Laboratories (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions)

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AF594 (49 citations)

3 protocols using goat anti rat af594

1

Immunohistochemical Analysis of Spinal Cord

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After blocking with 10% goat serum (Vector Laboratories), spinal cord sections were incubated with antibodies for APC (1:100, clone CC1, Abcam), Ki67 (1:200, clone SoIA15, eBioscience), and Olig2 (1:200; cat. no. AB9610, Millipore) overnight. Secondary antibodies goat anti-rabbit AF488 (1:200; cat. no. A-11008, Life Technologies) and goat anti-rat AF594 (1:200; cat. no. A-11007, Life Technologies) were incubated for 1 hr at RT. For CC1 detection acidic antigen retrieval was performed and a mouse-on-mouse immunodetection kit was used (Vector Laboratories). Nuclei were counterstained with DAPI before coverslipping with ProLong Gold Antifade (Life Technologies). Spinal cord images were acquired on a Leica DM5500 epifluorescence microscope.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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2

Immunohistochemical Analysis of Spinal Cord

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After blocking with 10% goat serum (Vector Laboratories), spinal cord sections were incubated with antibodies for APC (1:100, clone CC1, Abcam), Ki67 (1:200, clone SoIA15, eBioscience), and Olig2 (1:200; cat. no. AB9610, Millipore) overnight. Secondary antibodies goat anti-rabbit AF488 (1:200; cat. no. A-11008, Life Technologies) and goat anti-rat AF594 (1:200; cat. no. A-11007, Life Technologies) were incubated for 1 hr at RT. For CC1 detection acidic antigen retrieval was performed and a mouse-on-mouse immunodetection kit was used (Vector Laboratories). Nuclei were counterstained with DAPI before coverslipping with ProLong Gold Antifade (Life Technologies). Spinal cord images were acquired on a Leica DM5500 epifluorescence microscope.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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3

Multicolor Immunofluorescence Staining of Synovium

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Snap-frozen synovium biopsies were cut into 5-μm slices and mounted onto Menzel-Gläser SuperFrost slides and stored at − 20 °C. Briefly, slides were thawed, fixed in cold acetone, blocked with TNB (0.1 M Tris–HCl, 0.15 M NaCl, 0.5% blocking agent; Roche, Basel, Switzerland), stained with primary and secondary antibodies separately and embedded with ProLong Gold Antifade (Life Technologies). The panel included rat anti-human CD3 (CD3-12; Biorad, Hercules, CA, USA), mouse anti-human Vα7.2 (IgG1, 3C10; Biolegend), rabbit anti-human CD20 (RB-9013-P; Life Technologies, ThermoFisher Scientific), mouse anti-human CD161 (IgG2a, 191B8; Miltenyi) and Hoechst 33342 (50 μg/ml; Life Technologies). Secondary antibodies included goat anti-rat-AF594, goat anti-mIgG1-AF488, goat anti-rabbit-AF647 and goat anti-mIgG2a-AF546 (Life Technologies). Acquisition of images was done on a Leica TCS SP8 X White Light Laser with a 63× oil objective using Leica Acquisition Suite X software (Leica Microsystems B.V, Amsterdam, the Netherlands).
Koppejan H., Jansen D.T., Hameetman M., Thomas R., Toes R.E, & van Gaalen F.A. (2019). Altered composition and phenotype of mucosal-associated invariant T cells in early untreated rheumatoid arthritis. Arthritis Research & Therapy, 21, 3.
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