Cd11b bv510

Manufactured by BD

Sourced in United States

CD11b-BV510 is a fluorescently labeled antibody that binds to the CD11b surface antigen. CD11b is a cell adhesion molecule expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. The BV510 fluorophore is used to label the antibody, enabling its detection and quantification in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Cd8 pe cy7, by BD (3 mentions) Facscanto, by BD (3 mentions) Ly6g percp cy5, by BD (3 mentions) Cd8 bv510, by BD (2 mentions) Cd19 percp cy5, by BD (2 mentions) Ly6c fitc, by BD (2 mentions) Cd4 bv421, by BD (2 mentions) Fc block, by Thermo Fisher Scientific (2 mentions) Cd45 apc cy7, by BD (2 mentions) Cd19 apc, by BD (2 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (2 mentions) Cd45 pb, by BD (2 mentions) Ly6c pe, by BD (2 mentions) Ly6g fitc, by BD (2 mentions) Fortessa x20, by BD (1 mentions) Fortessa1, by BD (1 mentions) Cd4 apc cy7, by BD (1 mentions) Cd11c pe cy7, by BD (1 mentions) Cd279 pe, by BD (1 mentions) Cd69 bv421, by BD (1 mentions)

Close spelling variants of this product

CD11b (454 citations) Anti-CD11b-BV510 (6 citations) Anti-mouse CD11b-BV510 (2 citations) CD11B/C-BV510 (2 citations)

12 protocols using cd11b bv510

Comprehensive Immune Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

1 × 10⁶ splenocytes were taken for antibody staining and downstream analysis. Specific monoclonal antibodies were purchased from eBioscience (Thermofisher Scientific, Waltham, MA, USA) or BD Biosciences (San Jose, CA, USA): CD4 APC-Cy7 (RM4-5), CD8 BV510 (53-6.7), CD11b BV510 (M170), CD11c PECy7 (HL3), CD19 PerCP-Cy5.5 (ID3), CD69 BV421 (H1.2F3), CD279 PE (PD1; J43), Gr-1 Alexa700 (Ly6G/Ly6C; RB6-8C5), MHC-II FITC (I-A/I-E; 2G9) and CD16/32 (2.4G2). H-2D^b restricted LCMV tetramer staining and peptide restimulation were performed as previously described [20 (link)]. All flow cytometry data were collected on a Fortessa X20 or Fortessa1 (BD Biosciences) and analyzed using FlowJo software (version 10, Flowjo LLC, Ashland, OR, USA).

Preston S.P., Allison C.C., Schaefer J., Clow W., Bader S.M., Collard S., Forsyth W.O., Clark M.P., Garnham A.L., Li-Wai-Suen C.S., Peiris T., Teale J., Mackiewicz L., Davidson S., Doerflinger M, & Pellegrini M. (2023). A necroptosis-independent function of RIPK3 promotes immune dysfunction and prevents control of chronic LCMV infection. Cell Death & Disease, 14(2), 123.

+ Open protocol

+ Expand

Immunophenotyping of Mouse Blood Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The heparinized blood from each animal was divided into two parts. A volume of 150 µl was designated for blood count and measured using an ABX Pentra 60 C+ hemoanalyzer (Horiba, Kyoto, Japan). The rest of the blood (200–300 µl) was lysed using EasyLyse™ (Dako, Glostrup, Denmark). The amount of cells in suspension was as determined by Turk’s solution (2% acetic acid; Sigma-Aldrich) using a hemocytometer chamber. Cells (5 × 10⁵/100 µl) were marked with two panels of monoclonal antibodies. The first panel was delineated to detect T, B, and NK lymphocytes (CD3ϵ, CD4, CD8, CD19, and NK1.1). The second panel was designed for determination of monocytes and neutrophils (CD11b, F4/80, Ly6C, and Ly6G). The following monoclonal antibodies with fluorochromes for flow cytometry analysis were purchased from BioLegend (San Diego, CA, United States): anti-mouse CD3ϵ–FITC, CD4–BV421, CD19–PE, CD11c–BV421, CD45–APC/Fire™750, F4/80–PE, and from BD Bioscience: CD8–PECy7, CD11b–BV510, Ly6C–FITC, Ly6G–PECy7, and NK1.1–APC.

Lierova A., Kasparova J., Pejchal J., Kubelkova K., Jelicova M., Palarcik J., Korecka L., Bilkova Z, & Sinkorova Z. (2020). Attenuation of Radiation-Induced Lung Injury by Hyaluronic Acid Nanoparticles. Frontiers in Pharmacology, 11, 1199.

+ Open protocol

+ Expand

Mycobacterium tuberculosis Infection in Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were infected with H37Rv Mtb-mCherry and sacrificed at D10 and D14. Lungs were excised and submerged in BD Cytofix fixative solution diluted 1:3 with PBS for 24hr at 4°C. Lungs were washed 2x in PBS and dehydrated in 30% sucrose for 24hr prior to OCT embedding and rapid freezing in a methylbutane-dry ice slurry. 20um sections were stained overnight at RT (with the following antibodies: CD11c BV480; HL3 (BD), CD11b BV510; M1/70 (BD), CD45.2 AF700; 104 (BioLegend), MHC-II APC-Fire750; M5/114.15.2 (BioLegend), Siglec-F BV421; E50-2440 (BD), pS6 AF488; 2F9 (Cell Signaling), CD3 CF633; 17A2, CD4 CF660C; RM4-5) and coverslipped with Fluoromount G mounting media (Southern Biotec). Images were acquired on a Leica SP8X confocal microscope, compensated for fluorophore spillover using LAS X (Leica), and analyzed with Imaris (Bitplane) and FlowJo (11 (link)). More details can be found in Supplemental Fig. 3.

Delahaye J.L., Gern B.H., Cohen S.B., Plumlee C.R., Shafiani S., Gerner M.Y, & Urdahl K.B. (2019). Bacillus Calmette-Guerin-induced T cells shape Mycobacterium tuberculosis infection before reducing the bacterial burden. Journal of immunology (Baltimore, Md. : 1950), 203(4), 807-812.

+ Open protocol

+ Expand

Multiparametric flow cytometry for immune cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood was mixed with PBS/5mM EDTA and spun down. Lung tissue was digested as described above. After RBC lysis with PharmLyse (BD), resuspended leukocytes were incubated with F_c block (eBioscience) in FACS buffer for 10 min; followed by staining with antibodies: CD45-APC-Cy7, CD11b-PE-Cy7, Ly6C-FITC, Ly6G-PerCP-Cy5.5, CD45-PerCP-Cy5.5, CD3-APC-Cy7, CD4-FITC, CD8-PE-Cy7, CD11b-BV510, CD19-APC, NK1.1-PerCP-Cy5.5, CD45-PB, anti-CD11b-APC-Cy7, anti-Ly6C-APC (all from BD) and CD146-PerCP-Cy5.5 (Biolegend) for 30 min on ice. For staining of CCR2 we used MC-21 antibody (30 (link)), followed by goat-anti-rat-PE antibodies (BD). Data were acquired with a FACSCanto (BD), in some cases with CountBright absolute counting beads (Life Technologies), and analyzed by FlowJo software (Tree Star).

Roblek M., Protsyuk D., Becker P.F., Stefanescu C., Gorzelanny C., Glaus Garzon J.F., Knopfova L., Heikenwalder M., Luckow B., Schneider S.W, & Borsig L. (2018). CCL2 is a vascular permeability factor inducing CCR2-dependent endothelial retraction during lung metastasis. Molecular cancer research : MCR, 17(3), 783-793.

+ Open protocol

+ Expand

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was performed on primary cells obtained from explanted organs. Spleens, lungs, primary tumors, and livers were minced and enzyme digested with Liberase TL or TM (Roche), then filtered through a 70 μm cell strainer (Corning) to obtain a cell suspension. Whole blood was collected by cardiac puncture using a 23 guage needle (BD) attached to a 1 mL BD Luer-Lok syringe and mixed with 10 v/v% 25 mM EDTA (Life Technologies). Red blood cell lysis of whole blood, spleens, and lungs was performed with ACK Lysing Buffer (Life Technologies). Cells were pelleted by centrifugation at 400 × g for 5 minutes and resuspended in MACS buffer. NP⁺ cells were identified by Cy5.5 fluorescence signal. Nonspecific staining was blocked with anti-CD16/32 (Biolegend) and samples were stained for innate immune markers with anti-mouse CD45 AF700, F4/80 PE-Cy7, Ly6G PacBlue, Ly6C FITC (Biologend), and CD11b BV510 (BD Biosciences). For adaptive immune markers, samples were stained with anti-mouse CD45 AF700, CD4 V500, CD8 FITC, CD19 PacBlue, and CD49b PE-Cy7 (Biolegend). Stained samples were analyzed using the MoFlo Astrios Flow Cytometer or CytoFLEX (Beckman Coulter), and data were processed using FlowJo (BD) with the gating strategies shown in Figure S1.

Zhang Y., Hughes K.R., Raghani R.M., Ma J., Orbach S., Jeruss J.S, & Shea L.D. (2021). Cargo-free immunomodulatory nanoparticles combined with anti-PD-1 antibody for treating metastatic breast cancer. Biomaterials, 269, 120666.

+ Open protocol

+ Expand

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood cells were collected from the eye vein of anesthetized mice, and splenocytes from the spleen after filtration through cell strainers (70 µm). Ammonium-chloride-potassium lysing buffer was used for the lysis of red blood cells. Blood cells were labeled for 15 min at 4°C with fluorochrome-conjugated monoclonal antibodies: CD45-FITC BD Biosciences Clone 30-F11 (1:100), Ly6G BV421 BioLegend Clone 1A8 (1:500), Ly6C BV605 BD Biosciences Clone AL-21 (1:500), CD3e BV786 BD Biosciences Clone 5OOA2 (1:500), CD45R (B220) PE BD Biosciences Clone RA3-6B2 (1:500) and CD11b PE-Cy7 eBioscience Clone M1/70 (1:500). Splenocytes were labeled for 15 min at 4°C with fluorochrome-conjugated monoclonal antibodies: CD3 BV786 BD Biosciences (1:500), CD11b BV510 BD Biosciences (1:500), CD49b PE eBioscience (1:500), CD4 BV650 BD Biosciences (1:500), CD25 FITC BioLegend (1:500) and Foxp3 PerCP-Cy5.5 eBioscience (1:100). Samples were acquired with a BD LSRII Fortessa (BD Biosciences) and analyzed with FlowJo software.

Cordero-Sanchez C., Riva B., Reano S., Clemente N., Zaggia I., Ruffinatti F.A., Potenzieri A., Pirali T., Raffa S., Sangaletti S., Colombo M.P., Bertoni A., Garibaldi M., Filigheddu N, & Genazzani A.A. (2019). A luminal EF-hand mutation in STIM1 in mice causes the clinical hallmarks of tubular aggregate myopathy. Disease Models & Mechanisms, 13(2), dmm041111.

+ Open protocol

+ Expand

Tumor Single-Cell Isolation and Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

After intracardiac injection of PBS, breast cancer tissues, lung metastases or lungs were harvested, minced and digested at 37°C for 45 min with DMEM medium containing collagenase type 1A (1.5 mg/ml), hyaluronidase (1.5 mg/ml), and DNase (20 U/ml). The digestion mixtures were filtered through 70 μm cell strainers. Single-cell suspensions were incubated with rat anti-mouse CD16/CD32 mAb (BD Biosciences), and then stained, washed and re-suspended in cold buffer (1%BSA, 0.1% NaN3 in PBS). 7AAD reagent (eBioscience) was added to the stained tubes (5 μl/tube) just before running the flow analysis. Flow cytometry data were acquired on a Gallios flow cytometer (Beckman, USA), and data were analyzed with Kaluza software (version 1.3). The appropriate, fluorochrome-conjugated, isotype-matched, control IgGs were used in all experiments. The following monoclonal anti-mouse antibodies were used: CD45-PE-Cy7, CD45-PerCP, CD45-BV421, Gr1-PerCP-Cy5.5, Gr1-APC, Gr1-APC-Cy7, CD11b-APC-Cy7, CD11b-BV510, Ly-6G-FITC, Ly-6C-PE (BD Biosciences) and F4/80-PE, F4/80-FITC, F4/80-APC (eBioscience).

Fang Z., Wen C., Chen X., Yin R., Zhang C., Wang X, & Huang Y. (2017). Myeloid-derived suppressor cell and macrophage exert distinct angiogenic and immunosuppressive effects in breast cancer. Oncotarget, 8(33), 54173-54186.

+ Open protocol

+ Expand

Comprehensive Immunological Assays for In-Depth Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gum arabic, activated carbon, 2,7-dichlorofluorescein diacetate (DCFDA), toluidine blue, protease inhibitor cocktail tablets, phosphatase inhibitor cocktail tablets, MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel were purchased from Merck KGaA (Darmstadt, Germany). 25% Aqueous Solution Glutaraldehyde, Paraformaldehyde, Sorensen's Phosphate Buffer, 2% Aqueous Solution Osmium Tetroxide, Ethyl Alcohol, Acetone, Araldite, Dibutyl phthalate (DBP) were purchased Electron Microscopy Sciences (Hatfield, USA). Leukocyte Activation Cocktail with BD GolgiPlug™, FACS antibodies include anti-mouse I-A/I-E-BV510, IgG1-BB700, IgM-BV605, IgE-BV786, IgD-BV711, CD1d-BV421, CD5-PE, CD45-BUV395, CD19-APC, CD45R/B220-BUV496, CD45-APC-Cy7, CD3e-FITC, CD4-V450, CD8-BV510, CD25-BV605, IL-4-PE-Cy7, IFN-γ-PE, FoxP3-AF647, CD103-BUV395, F4/80-BV711, CD80-BV650, CD11b-BV510, Ly6-G-PerCP Cy5.5, PE-Ly6-C, CD45-APC-Cy7, CD11c--AF700 were purchased from BD (Heidelberg, Germany); Another FACS antibody Anti-mouse-IL-17A-BV650 was purchased from eBioscience (Frankfurt am Main, Germany). Anti-mouse HMGB1, Anti-mouse phospho-p65, anti-mouse phospho-p38, anti-mouse GAPDH and anti-rabbit IgG HRP were obtained from Cell signaling Technology (Frankfurt am Main, Germany). IgE and histamine ELISA kits were purchased from Abcam (Berlin, Germany).

Lin S., Kühn F., Schiergens T.S., Zamyatnin AA J.r., Isayev O., Gasimov E., Werner J., Li Y, & Bazhin A.V. (2021). Experimental postoperative ileus: is Th2 immune response involved?. International Journal of Medical Sciences, 18(13), 3014-3025.

+ Open protocol

+ Expand

Profiling Tumor-Associated Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The lungs of 4T1-tumor bearing mice were perfused, minced and digested in Collagenase D and A (2 mg each, Roche) for 1 hour at 37°C. A single-cell suspension using 40μm cell strainers was prepared, erythrocytes lysed. Cells were resuspended in staining buffer with F_c block (eBioscience), incubated on ice for 10 min, followed by staining with following antibodies: CD45-PB, CD11b-BV510, CD64-PE, Ly6C-APC, Ly6G-PerCP-Cy5.5 (all from BD) for 30 min. Data were collected with CountBright absolute counting beads (Life Technologies) using a FACSCanto (BD) and analyzed by FlowJo software (FlowJo, LCC).

Knopfová L., Biglieri E., Volodko N., Masařík M., Hermanová M., Garzón J.G., Dúcka M., Kučírková T., Souček K., Šmarda J., Beneš P, & Borsig L. (2017). Transcription factor c-Myb inhibits breast cancer lung metastasis by suppression of tumor cell seeding. Oncogene, 37(8), 1020-1030.

+ Open protocol

+ Expand

Immune Cell Analysis in Aged Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

To analyze immune cells (innate and adaptive) in the spleen, bone marrow or inguinal lymph nodes of 6- and 12-month-old Mlkl^WT/WT, Mlkl^–/–, Ripk3^WT/WT, and Ripk3^–/– mice, single-cell suspensions were subjected to osmotic red blood cell lysis and incubated with a combination of antibodies: CD4-BV421 (BD Biosciences #562891), CD8-PeCy7 (BD Biosciences #561097), CD19-PerCPCy5.5 (BD Biosciences #551001), CD11b-BV510 (BD Biosciences #562950) or CD11b-BV786 (BD Biosciences #740861), CD45-Alexa700 (BD Biosciences #560566), Ly6G-V450 (BD Biosciences #560603) and Ly6C-APCCy7 (BD Biosciences #560596). Samples were analyzed on an Aurora Cytex flow cytometer, with the automated volume calculator used to quantify absolute cell numbers. FlowJo v10.8 was used for all analyses.

Tovey Crutchfield E.C., Garnish S.E., Day J., Anderton H., Chiou S., Hempel A., Hall C., Patel K.M., Gangatirkar P., Martin K.R., Li Wai Suen C.S., Garnham A.L., Kueh A.J., Wicks I.P., Silke J., Nachbur U., Samson A.L., Murphy J.M, & Hildebrand J.M. (2023). MLKL deficiency protects against low-grade, sterile inflammation in aged mice. Cell Death and Differentiation, 30(4), 1059-1071.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!