The human dopaminergic neuroblastoma SH-SY5Y cell line was purchased from the Shanghai Institutes for Biological Sciences. Bupivacaine hydrochloride (purity 99.9%), glucose (purity 99.5%), and N-acetyl-L-cysteine (NAC) were purchased from Sigma (St. Louis, MO). Other reagents used included Dulbecco's modified Eagle medium (DMEM)/F12 (including 17.5 mM glucose) and fetal bovine serum (FBS: Gibco, Grand Island, NY); Cell Counting Kit-8 (CCK8) assay kit (Dojindo, Kumamoto, Japan); 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (Beyotime, China); 4′,6-diamidino-2-phenylindole dihydrochloride n-hydrate (DAPI) which were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan); comet assay (Trevigen, Inc., Gaithersburg, MD); anti-OGG1 and anti-8-oxodG (Abcam, Cambridge, UK, ab115841 and ab62623); anti-caspase-9 (CST, USA, 9508) and anti-β-actin (KangChen Bio-tech, China, KC-5A08). All reagents were obtained from commercial suppliers and were of standard biochemical quality.
Anti β actin
Manufactured by Kangchen Biotech
Sourced in United States
Anti-β-actin is a primary antibody that specifically recognizes the β-actin protein, a ubiquitous and highly conserved cytoskeletal protein found in eukaryotic cells. It is commonly used in Western blotting and immunocytochemistry applications to detect and quantify the expression of β-actin as a loading control or reference standard.
Automatically generated - may contain errors
Lab products found in correlation
Bupivacaine hydrochloride, by Merck Group (2 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Beyotime (2 mentions)
4 6 diamidino 2 phenylindole dihydrochloride n hydrate dapi, by Fujifilm (1 mentions)
Cell counting kit 8 cck 8 assay kit, by Dojindo Laboratories (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
N acetyl l cysteine, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rhod 2 acetoxymethyl ester rhod 2 am, by Thermo Fisher Scientific (1 mentions)
Ru360, by Merck Group (1 mentions)
Anti mcu, by Abcam (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Anti p62, by Cell Signaling Technology (1 mentions)
Anti il 18, by Cell Signaling Technology (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Anti parkin, by Cell Signaling Technology (1 mentions)
Anti nlrp3, by Cell Signaling Technology (1 mentions)
Anti rab7, by Cell Signaling Technology (1 mentions)
Anti caspase 1 p20, by Santa Cruz Biotechnology (1 mentions)
Anti pink1, by Cell Signaling Technology (1 mentions)
3 protocols using anti β actin
1
Bupivacaine-Induced Oxidative Stress in SH-SY5Y Cells
2
Bupivacaine-Induced Mitochondrial Dysfunction
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The SH-SY5Y cell line was purchased from Shanghai Institutes for Biological Sciences (Shanghai, China). Dulbecco's modified Eagle medium (DMEM)/F12 (including 17.5 mM glucose) and fetal bovine serum were purchased from Gibco company (Grand Island, NY, USA). Bupivacaine hydrochloride, glucose, Ru360, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rhod-2-acetoxymethyl ester (Rhod-2/AM) was purchased from Invitrogen (Carlsbad, CA, USA). Other reagents are the following: 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (Beyotime, Haimen, China), propidium iodide and annexin V-fluorescein isothiocyanate (FITC) (KeyGEN, Nanjing, China), anti-MCU (Abcam, Cambridge, MA, USA), and anti-β-actin (KangChen Bio-tech, Shanghai, China).
3
NLRP3 Inflammasome Pathway Regulation
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Drugs such as MCC950 (Sigma-Aldrich, St Louis, USA), caspase-1 inhibitor AC-YVAD-CMK (Sigma-Aldrich), FBXL2 activator BC-1258 (Sigma-Aldrich), and FBXL2 inhibitor GGTI-2418 (Med Chem Express, Princeton, USA) were applied to mice or cardiomyocytes. Anti-NLRP3 (1:1000; Cell Signaling Technology, Danvers, USA), anti-caspase-1 p20 (1:100; Santa Cruz Biotechnology, Santa Cruz, USA), anti-IL-1β (1:1000; Cell Signaling Technology), anti-IL-18 (1:1000; Cell Signaling Technology), anti-Pink1 (1:1000; Cell Signaling Technology), anti-Parkin (1:1000; Cell Signaling Technology), anti-p62 (1:1000; Cell Signaling Technology), anti-LC3B (1:1000; Cell Signaling Technology), anti-Rab7 (1:1000; Cell Signaling Technology), anti-Bax (1:500; Cell Signaling Technology), anti-Bcl-2 (1:500; Cell Signaling), anti-FBXL2 (1:2000; Novus Biologicals, Littleton, USA), anti-GAPDH (1:1000; Cell Signaling Technology), anti-β-actin (1:5000; KangChen Biotech, Shanghai, China), and anti-rabbit or anti-mouse secondary antibody (1:5000; Proteintech, Chicago, USA) were used in western blot analysis.
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