Antibodies against phospho smad2 3

Manufactured by Cell Signaling Technology

Sourced in United States

Antibodies against phospho-smad2/3 are laboratory reagents used to detect and measure the levels of phosphorylated Smad2 and Smad3 proteins. These proteins are key components of the TGF-beta signaling pathway, which plays a crucial role in various cellular processes, such as cell growth, differentiation, and apoptosis. The antibodies can be used in techniques like Western blotting, immunohistochemistry, and ELISA to analyze the activation state of the TGF-beta pathway in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using antibodies against phospho smad2 3

TGF-β1 Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human TGF-β1 was purchased from ProSpec (East Brunswick, NJ, USA). Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM), and RPMI 1640 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Bouin's solution, Giemsa solution, and 4′,6-diamidino-2-phenylindole were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against phospho-smad2/3, phospho-p38, phospho-ERK, smad2/3, p38, ERK, Snail, and E-cadherin were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Kee J.Y., Han Y.H., Mun J.G., Park S.H., Jeon H.D, & Hong S.H. (2017). Effect of Korean Red Ginseng extract on colorectal lung metastasis through inhibiting the epithelial–mesenchymal transition via transforming growth factor-β1/Smad-signaling-mediated Snail/E-cadherin expression. Journal of Ginseng Research, 43(1), 68-76.

+ Open protocol

+ Expand

Immunoblotting Analyses of Liver Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot assays were performed using whole liver lysates or membrane extracts of the liver samples as described previously (26 (link), 27 (link)). Antibodies against HNF4α (cat # sc-374229) or p53 (cat # sc-6243) were purchased from Santa Cruz Biotechnology. Antibodies against CYP7A1 (cat # TA351400) or CYP8B1 (cat # TA313734) were purchased from Origene. Antibodies against SR-BI (cat # NB400–101) or Calnexin (cat # NB100–1965) were purchased from Novus. Antibodies against phospho-Smad2/3 (cat # 8828), total Smad2/3 (cat # 5678), cleaved Caspase 3 (cat # 9661) or total Caspase-3 (cat # 9662) were purchased from Cell Signaling Technology. Antibodies against CES1 (cat # ab45957), CES2 (cat # ab56528), ACAT2 (cat # ab23669) or Tubulin (cat # ab4074) were purchased from Abcam.

Xu Y., Zhu Y., Hu S., Xu Y., Stroup D., Pan X., Bawa F.C., Chen S., Gopoju R., Yin L, & Zhang Y. (2021). Hepatocyte nuclear factor 4α prevents the steatosis-to-NASH progression by regulating p53 and bile acid signaling. Hepatology (Baltimore, Md.), 73(6), 2251-2265.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!