C6h12o6 h2o

The surface of the SiO₂@ZrO₂ core–shell material was modified by solvo-thermal synthesis [47 (link)]. The SiO₂@ZrO₂ structures were stirred with ethanol absolute reagent for 15 min, and this mixture was named dissolution 1. C₆H₁₂O₆∙H₂O (2.00 g, Merck) and H₂NCONH₂ (0.440 g, Merck) were dissolved in HPLC water grade Merck (20 mL), and 0.250g of Ca(NO₃)₂·4H₂O Sigma-Aldrich precursor was incorporated over the solution. The resulting mixture was kept for 15 min under stirring, and this mixture was named dissolution 2. After that, dissolution 2 was added dropwise into dissolution 1 under stirring for an additional 15 min. The formed nanoparticles were transferred into a Teflon-lined stainless-steel autoclave Parr reactor and maintained for 20 h at 160 °C. The material was washed several times in ethanol absolute and dried at 70 °C for 12 h, and then the solid was calcined at 700 °C in air for 6 h.

Acetylcholine, atropine, modified Tyrode's Salt Solution, and Nω-nitro-L-arginine methyl ester hydrochloride were acquired from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA); NaCl, KCl, CaCl₂·6H₂O, MgCl₂·6H₂O, NaHCO₃, NaH₂PO₄, and C₆H₁₂O₆·H₂O were purchased from Merck (Darmstadt, Germany). Phenylephrine was acquired from AcrosOrganics (Geel, Belgium). The blockers CNCKAPETALCARRCQQH (apamin), XFTNVSCTTSKECWSVCQRLHNTSRGKCMNKKCRCYS (charybdotoxin), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), (9S,10R,12R)-2,3,9,10,11,12-hexahydro-10-methoxy-2,9-dimethyl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg:3′,2′,1′-kl]pyrrolo[3,4-i][1,6]benzodiazocine-10-carboxylic acid, and methyl ester (KT5823) were obtained from Tocris Bioscience (Bristol, UK).