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Gtx100015

Manufactured by GeneTex
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The GTX100015 is a laboratory equipment device. It is designed for scientific research applications.

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Lab products found in correlation

Can get signal solution 1, by Toyobo (2 mentions) Chemidoc xrs system with image lab software, by Bio-Rad (2 mentions) Immun star westernc chemiluminescence kit, by Bio-Rad (2 mentions) Can get signal solution 2, by Toyobo (2 mentions) Can get signal kit, by Toyobo (2 mentions) Hrp conjugated goat polyclonal antibody against rabbit igg, by Bio-Rad (2 mentions)

2 protocols using gtx100015

1

GRPR Western Blotting Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was conducted according to our previously described methods (Takanami et al., 2016 (link)). Membranes were blocked with the PVDF blocking reagent from the Can Get Signal kit (Toyobo) for 30 min at room temperature and then incubated overnight at 4°C in Can Get Signal Solution 1 (Toyobo) containing a 1:1,000 dilution of rabbit polyclonal antibody against human GRPR (GTX100015, GeneTex). Blotted membranes were washed three times with 0.05% Tween 20 in Tris-HCI buffered saline (TBST) and incubated with HRP-conjugated goat polyclonal antibody against rabbit IgG (Bio-Rad Laboratories) at a 1:10,000 dilution in Can Get Signal Solution 2 (Toyobo) for 1 h at room temperature. After washing five times with TBST, blots were visualized by the Immun-Star WesternC Chemiluminescence Kit (Bio-Rad Laboratories). Images of blots were detected by ChemiDoc XRS+ System with Image Lab Software (Bio-Rad Laboratories) and adjusted slightly for brightness and contrast to provide a uniform background.
Takanami K., Oti T., Kobayashi Y., Hasegawa K., Ito T., Tsutsui N., Ueda Y., Carstens E., Sakamoto T, & Sakamoto H. (2022). Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans. The Journal of comparative neurology, 530(16), 2804-2819.
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2

GRPR Western Blotting Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was conducted according to our previously described methods (Takanami et al., 2016 (link)). Membranes were blocked with the PVDF blocking reagent from the Can Get Signal kit (Toyobo) for 30 min at room temperature and then incubated overnight at 4°C in Can Get Signal Solution 1 (Toyobo) containing a 1:1,000 dilution of rabbit polyclonal antibody against human GRPR (GTX100015, GeneTex). Blotted membranes were washed three times with 0.05% Tween 20 in Tris-HCI buffered saline (TBST) and incubated with HRP-conjugated goat polyclonal antibody against rabbit IgG (Bio-Rad Laboratories) at a 1:10,000 dilution in Can Get Signal Solution 2 (Toyobo) for 1 h at room temperature. After washing five times with TBST, blots were visualized by the Immun-Star WesternC Chemiluminescence Kit (Bio-Rad Laboratories). Images of blots were detected by ChemiDoc XRS+ System with Image Lab Software (Bio-Rad Laboratories) and adjusted slightly for brightness and contrast to provide a uniform background.
Delacrétaz E., Michalopoulos P.G., Ruiz J., Saner H, & Meier B. (1998). Management of hyperlipidaemia after coronary revascularisation: follow up study. BMJ : British Medical Journal, 316(7143), 1499-1500.
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