ER+ human breast cancer cell lines (MCF-7) were from ATCC (University Boulevard, Manassas, VA, USA). Cell culture medium DMEM/F12, Lipofectamine 2000, TRIzol reagent and OPTI-MEM were from Life Technology (Van Allen Way, Carlsbad, CA, USA). Antibodies against Runx2 and b-actin and siRNA of Runx2 were obtained from Santa Cruz (Santa Cruz, CA, USA). Brf1 antibody was from Bethyl laboratories Inc (West FM, Montgomery, TX, USA). JNK inhibitor, SP600125 was from A.G. Scientific, Inc (Lusk Blvd, San Diego, CA, USA). The siRNAs of c-Jun and Runx2 were bought from Santa Cruz (Santa Cruz, CA, USA). The sequences of JNK1 and Brf1 siRNA (Table S1 ) and primers of Pol III genes (Table S2 ) were listed in Supplements (36 (link)–37 (link)). E2 (17b-estradiol) was from Sigma-Aldrich (St. Louis, MO, USA). Plasmid of Runx2 expression and Runx2-luciferase reporter construct were kindly provided by Dr. Baruch Frenkel (University of Southern California, USA) (38 (link)).
Brf1 antibody
Manufactured by Fortis Life Sciences
Sourced in United States
The Brf1 antibody is a laboratory reagent used for the detection and analysis of the Brf1 protein. Brf1 is a subunit of the RNA polymerase III transcription initiation complex and plays a role in the regulation of gene expression. The Brf1 antibody can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression, localization, and interactions of the Brf1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Trizol reagent, by Thermo Fisher Scientific (3 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Mcf 10f, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Histone h3 antibody, by Cell Signaling Technology (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Mcf 10 2a, by American Type Culture Collection (1 mentions)
H1975, by American Type Culture Collection (1 mentions)
Dorsomorphin compound c 2hcl, by Selleck Chemicals (1 mentions)
Aml 12 cell line, by American Type Culture Collection (1 mentions)
Antibody against β actin, by Santa Cruz Biotechnology (1 mentions)
4 protocols using brf1 antibody
1
Regulation of Runx2 in ER+ Breast Cancer
2
Breast Cancer Cell Lines Characterization
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ER- human breast non-tumorigenic epithelial cell lines (MCF-10A, MCF-10F and MCF-10-2A), ER+ human breast cancer cell lines (MCF-7 and T-47D) and ER- human breast cancer cell lines MDA-MB231 and SKBR-3) were from ATCC (Manassas, Virginia, USA). Tamoxifen was from Sigma-Aldrich. Cell culture medium (DMEM/F12), OPTI-MEM, Lipofectamine 2000 and TRIzol reagent were from Life Technologies (San Diego, CA, USA). Antibodies against β-actin and TFIIIC63 and c-Jun siRNA (Catalog No. SC-29224) were obtained from Santa Cruz Biotech (Santa Cruz, CA, USA). Mismatch RNA was described previously [24 (link)]. Histone H3 antibody were from Cell Signaling (Danvers, MA, USA). Brf1 antibody was from Bethyl laboratories Inc (Montgomery, TX, USA). The sequences of primers were described in (Supplements) [8 (link), 30 (link)].
3
Lung Cancer Cell Line Characterization
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The lung cancer cell lines A549 and H1975, and normal human bronchial epithelial cell line 16HBE were purchased from the American Type Culture Collection (Manassas, VA) and cultured in RPMI 1640 containing 10% FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin (HyClone, Utah, USA). All cells were tested for negative mycoplasma contamination and authenticated based on short tandem repeat fingerprinting before use. AMPK inhibitor, S7306 (Dorsomorphin (Compound C) 2HCl, Cat No. S7306), was purchased from Selleck and dissolved in sterile water. N-Methyl-N′-nitro-N-nitrosoguanidine (MNNG) was purchased from Accu. Standard, Inc (Cat No. R-081N) and dissolved in DMSO. Brf1 antibody was from Bethyl Laboratories Inc (Cat No. A301-228A). The MTT assay kit was from Boster Biotech (Cat No. AR1156).
4
Comprehensive Liver Cell Culture Protocol
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Cell culture medium (DMEM/F12), OPTI-MEM, Lipofectamine 2000 and TRIzol reagent were from Life Technologies (San Diego, CA, USA). Antibody against β-actin was obtained from Santa Cruz Biotech (Santa Cruz, CA, USA). Mismatch RNA (mmRNA) was described previously [16 (link)]. Brf1 antibody was from Bethyl laboratories Inc (Montgomery, TX, USA). The sequences of primers and Brf1 siRNA were described in (Supplementary Table S3 ) [20 (link)]. Tumor stem cells of mouse liver (TSCML) were kindly provided by Dr. K. Machida at USC (University of Southern California). AML-12 cell line was from ATCC. HepG2-ADH cell line was kindly provided by Dr. D.L. Clemens (University of Nebraska). The animal experiments were conducted in accordance with the approved institutional animal care and use committee protocol at USC.
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