572 2 infrared thermometer

Four‐week‐old male C57BL/6J mice were purchased at weaning from the Jackson Laboratory (Stock #000664; Bar Harbor, ME, USA). All mice were single‐housed and maintained in a conventional animal facility at 21–22°C at a relative humidity of 50 ± 10% on a 12‐h light:dark cycle from time of weaning. All mice were fed a diet consisting of 60% kcal from fat, 20% kcal from protein, and 20% kcal from carbohydrate (VHFD; cat #D12492, Research Diets, New Brunswick, NJ, USA). Animals randomized to BAM15 were fed a diet consisting of 60% kcal from fat, 20% kcal from protein, and 20% kcal from carbohydrate with the addition of 0.1% w/w BAM15 (Research Diets). Each animal was given ad libitum access to food (maintaining approximately 50 g of food in the hopper) and water unless indicated. Prior to treatment allocation or experimentation, each animal was weighed twice weekly and body temperature was recorded via infrared thermometry during weighing (Fluke 572‐2 Infrared Thermometer; Fluke, Everett, WA, USA). All experiments and procedures involving animals were approved by the Institutional Animal Care and Use Committee of Pennington Biomedical Research Center (permit number 1048).

Body temperature was recorded via infrared thermometry during weighing (Fluke 572-2 Infrared Thermometer; Fluke, Everett, WA, USA) as described previously [18 (link)].