Caco 2

Human mononuclear macrophage (THP-1) cells and human colorectal adenocarcinoma (Caco-2) cells were purchased from Shanghai Cell Bank. THP-1 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum, and Caco-2 cells were cultured in DMEM medium containing 10% fetal bovine serum. Both of the cells were used in this study after stable passage to the sixth generation in the environment of 37 °C and 5% CO₂. The cells in the logarithmic growth phase were inoculated in a 6-well plate with 1 × 10⁶ cells in each well. The normal control group (NC), 5-FU group (5-FU), 5-FU + sodium acetate group (NaAc), 5-FU + sodium propionate group (NaPc) and 5-FU + sodium butyrate group (NaB) were set up in the experiment. The cells in the NC group were not do any treatment. The NaAc group, NaPc group and NaB group cells were pretreated with 100 μmol/L NaAc, NaPc, NaB for 24 h, respectively. The cells in the 5-FU group, NaAc group, NaPc group and NaB group were treated with 5-FU for 24 h. The concentration of 5-FU was 2.5 mmol/L in THP-1 cells and 5 mmol/L in Caco-2 cells. All methods were carried out in accordance with relevant guidelines and regulations.

The human colonic carcinoma cell lines LoVo, CaCo2, SW116, SW480 and HCT-116, and the normal colonic mucosa cell line NCM460 were purchased from the Shanghai Cell Bank, China. All cell lines were cultured in DMEM medium containing 10% FBS, 100 U/ml penicillin and 100 mg/ml streptomycin at 37 °C in a 5% CO₂ incubator. A939572 (#HY-50709), T0901317 (#HY-10626) both were purchased from MedChem Express, Shanghai, China.