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2 protocols using myricetin

1

Grape Pomace Extraction and HPLC Analysis

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The organic solvents for grape pomace extraction and HPLC analysis were HPLC grade (Fisher Scientific, Atlanta, GA). Intestinal acetone powders from rat, 4-nitrophenyl-α-d-glucopyranoside (pNPG), Folin–Ciocalteu reagent, and phenolic standards including caffeic acid, delphinidin chloride, gallic acid, malvin chloride, malvidin chloride, quercetin hydrate and quercetin 3-O-glucoside were purchased from Sigma-Aldrich (St. Louis, MO). Acarbose and other phenolic standards including catechin, epicatechin gallate, kaempferol, myricetin and resveratrol were obtained from LKT Laboratories, Inc. (St. Paul, MN). Phenolic standards including cyanidin chloride and p-coumaric acid were purchased from Fluka Analytical (Buchs, Switzerland). Rutin was purchased from ACROS (Geel, Belgium).
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2

Comprehensive Chemical Reagents for Cellular Studies

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Resveratrol, piceatannol, resminostat, entinostat, mocetinostat, vorinostat, curcumin, garcinol, anacardic acid and Tip60i were purchased from Selleckchem (Houston, TX, USA). MB-3 and BMS-345541 were purchased from MilliporeSigma (Burlington, MA, USA). Pterostilbene and myricetin were from LKT Laboratories (St. Paul, MN, USA) and trimethoxy-Resveratrol (trans-3,5,4′-trimethoxystilbene) was from Cayman Chemical Co. (Ann Arbor, MI, USA). Stock solutions of the chemicals were prepared based on the information provided by the manufacturer and maintained at −20°C. The antibodies for human PD-L1 (E1L3N, 13684), p38 MAPK (D13E1, 8690), NF-κB p65 (D14E12, 8242), γH2AX (20E3, 9718), cleaved caspase 3 (D3E9, 9579), IRF-1 (D5E4, 8478) and rabbit IgG isotype monoclonal antibody (DA1E, 5742) conjugated to PE were obtained from Cell Signaling Technology (Beverly, MA, USA). c-Myc antibody (9E10, sc-40) was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Fetal bovine serum, RPMI-1640, DMEM, streptomycin and penicillin were from Gibco/Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and solvents were of analytical grade.
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