Samples and reagents were weighed using an analytical balance ML 204T (Mettler Toledo, Columbus, OH, USA). The microwave-assisted acid digestion was performed in 100 mL closed Teflon vessels using an ETHOS PLUS microwave lab-station (Milestone, Sorisole, Italy). The total metal content was assessed using a NexION® 300X ICP-MS (Perkin Elmer, Waltham, MA, USA) equipped with a SeaFastSC2 DX autosampler (Elemental Scientific, Omaha, NB, USA). The nebulisation system consisted of a Meinhard® nebuliser and a cyclonic spray chamber thermostated by a Peltier refrigerator.
Ethos plus microwave lab station
Manufactured by Milestone
Sourced in Italy
The ETHOS PLUS microwave lab-station is a compact and versatile piece of laboratory equipment designed for sample preparation and analysis. It features a high-power microwave system that can be used for a variety of applications, including digestion, extraction, and synthesis. The unit is equipped with advanced safety features and is suitable for use in various research and industrial settings.
Automatically generated - may contain errors
Lab products found in correlation
Nexion 300x icp ms, by PerkinElmer (1 mentions)
Ml204t, by Mettler Toledo (1 mentions)
Agilent 8800 triple quadrupole icp ms, by Agilent Technologies (1 mentions)
Solaar m6, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Trace metal grade, by Thermo Fisher Scientific (1 mentions)
Concentrated hydrochloric acid, by Thermo Fisher Scientific (1 mentions)
Concentrated nitric acid, by Thermo Fisher Scientific (1 mentions)
4 protocols using ethos plus microwave lab station
1
Microwave-Assisted Elemental Analysis
2
Trace Metal Analysis in Biological Samples
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An Agilent 8800 Triple Quadrupole ICP-MS (ICP-QQQ, Agilent Technologies, Singapore) was used to determine the Cd and Tl content of biological samples. The apparatus was equipped with a MicroMist glass concentric nebulizer, a Peltier-cooled quartz double-pass Scott-type spray chamber, a quartz torch with a 2.5-mm i.d., Ni interface cones, an x-lens, and an octopole reaction system (ORS3). Samples were introduced directly into the ICP-MS using a SPS 4 autosampler with a standard peristaltic pump and tubing (i.d. 1.02 mm).
A Solaar M6 (Thermo Electron Corporation, Gloucester, UK) atomic absorption spectrometer, equipped with an electrothermal atomizer and a Zeeman background correction system, was used to determine the Cd concentration of liver samples.
A Milestone ETHOS Plus Microwave Labstation (Italy) was used for microwave-assisted digestion of samples. Samples were homogenized using a blender with a glass jar (Łucznik, Poland), a mechanical micro-stirrer (MPW-321; Mechanika Precyzyjna, Poland), and a SONOREX DIGIPLUS ultrasonic bath (DL 255H; ultrasonic nominal power: 160 W, power settings: 20–100%, ultrasonic frequency: 35 kHz) from Bandelin (Germany). A Memmert UE 400 oven (Germany) was used to dry biological samples. A DSX110 inverted microscope (Olympus Corporation, Japan) was used for measurements of puparial length and width.
A Solaar M6 (Thermo Electron Corporation, Gloucester, UK) atomic absorption spectrometer, equipped with an electrothermal atomizer and a Zeeman background correction system, was used to determine the Cd concentration of liver samples.
A Milestone ETHOS Plus Microwave Labstation (Italy) was used for microwave-assisted digestion of samples. Samples were homogenized using a blender with a glass jar (Łucznik, Poland), a mechanical micro-stirrer (MPW-321; Mechanika Precyzyjna, Poland), and a SONOREX DIGIPLUS ultrasonic bath (DL 255H; ultrasonic nominal power: 160 W, power settings: 20–100%, ultrasonic frequency: 35 kHz) from Bandelin (Germany). A Memmert UE 400 oven (Germany) was used to dry biological samples. A DSX110 inverted microscope (Olympus Corporation, Japan) was used for measurements of puparial length and width.
3
Ruthenium Bioaccumulation in Murine Organs
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Ruthenium content
in explanted organs (i.e., liver, kidneys, spleen, lungs, heart, brain,
prostate, and reproductive system) from mice treated withpnpRu-14 or PBS solution were evaluated by ICP–MS. Additionally, the
serum content of this metal was measured at fractions collected 0.5,
1.5, 4, 12, 36, and 48 h after administering the dose ofpnpRu-14 .
Optimized digestion of samples was carried out in a microwave
digestor (Ethos Plus Microwave Labstation, Milestone Inc., Monroe,
CT, USA) by placing 50 μL of serum, or a fragment of each organ
specimen previously weighed, in a Teflon digestion vessel where 2
mL of 20% HNO3 (diluted from 69% purity HNO3) was added. The vessels were closed, and the samples digested with
125 W of power per vessel using a four-stage program (stage 1 heating
to 200 °C for 5 min; stage 2 holding at 200 °C for 5 min;
stage 3 heating to 220 °C for 5 min; and a final step of 5 min
at 220 °C). Then, the vessels were allowed to cool to room temperature
for 15 min prior to opening the vessels. Digested solutions were then
adjusted with ultrapure water to obtain a 10 mL solution with the
correct acid concentration to perform the analysis. All the solutions
were prepared using ultrapure water obtained from a Millipore Milli-Q
system.
in explanted organs (i.e., liver, kidneys, spleen, lungs, heart, brain,
prostate, and reproductive system) from mice treated with
serum content of this metal was measured at fractions collected 0.5,
1.5, 4, 12, 36, and 48 h after administering the dose of
Optimized digestion of samples was carried out in a microwave
digestor (Ethos Plus Microwave Labstation, Milestone Inc., Monroe,
CT, USA) by placing 50 μL of serum, or a fragment of each organ
specimen previously weighed, in a Teflon digestion vessel where 2
mL of 20% HNO3 (diluted from 69% purity HNO3) was added. The vessels were closed, and the samples digested with
125 W of power per vessel using a four-stage program (stage 1 heating
to 200 °C for 5 min; stage 2 holding at 200 °C for 5 min;
stage 3 heating to 220 °C for 5 min; and a final step of 5 min
at 220 °C). Then, the vessels were allowed to cool to room temperature
for 15 min prior to opening the vessels. Digested solutions were then
adjusted with ultrapure water to obtain a 10 mL solution with the
correct acid concentration to perform the analysis. All the solutions
were prepared using ultrapure water obtained from a Millipore Milli-Q
system.
4
Quantitative Iron Analysis by AAS
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The iron content of the stock solutions was verified by AAS. Briefly, each stock solution (1 mL) was mixed with ultrapure water (3–4 mL), concentrated nitric acid (0.75 mL; trace metal grade; Fisher Scientific, Loughborrough, U.K.), and concentrated hydrochloric acid (0.15 mL; trace metal grade; Fisher Scientific, Loughborrough, U.K.) in Teflon microwave digestion vessels. The microwave digestion was then carried out in an Ethos plus microwave lab station (Milestone; Sorisole, Italy) at 180 °C for 15 min. Iron concentration in the microwave-digested samples was determined using an Agilent 240/280 Series AA spectrometer (Santa Clara, CA, USA).
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