Lsm 510 or 710 confocal microscope

Manufactured by Zeiss

Sourced in Germany

The LSM 510 or 710 confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It provides optical sectioning capabilities, allowing for the acquisition of high-resolution, three-dimensional images of samples. The instrument is equipped with a range of laser excitation sources and sensitive detectors to enable the visualization of a variety of fluorescent labels and samples.

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2 protocols using lsm 510 or 710 confocal microscope

Simultaneous Ligand Binding and Gating Measurement in Olfactory CNG Channels

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The ligand binding was measured in macropatches by patch-clamp fluorometry41 (link)42 combined with confocal microscopy26 (link)27 (link)43 (link). By means of fluorescently-labeled cGMP and cAMP derivatives, this technique allowed us to simultaneously measure ligand binding and gating in olfactory CNG channels. Recordings were performed with an LSM 510 or 710 confocal microscope (Zeiss, Jena, Germany) and were triggered by the ISO3 software (MFK, Niedernhausen, Germany). To distinguish between the fluorescence of the unbound ligands from that of the bound ligands, a second dye, DY647 (Dyomics, Jena, Germany), was added to the bath solution. The 543-nm and 633-nm lines of a He-Ne laser were used to excite fcAMP/fcGMP and DY647, respectively. The method of confocal patch-clamp fluorometry has been described in detail previously26 (link).

Nache V., Wongsamitkul N., Kusch J., Zimmer T., Schwede F, & Benndorf K. (2016). Deciphering the function of the CNGB1b subunit in olfactory CNG channels. Scientific Reports, 6, 29378.

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Laser-Mediated Axon Regeneration in C. elegans

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L4 stage hermaphrodites were immobilized with 0.05% levamisole in M9 buffer and mounted on 2% agar pads. Axons were severed using a femtosecond laser as described in (Wu et al., 2007 (link)). Animals were recovered and cultured on normal NGM plates at 20C and imaged 24 h later using 63X magnification on a Zeiss LSM 510 or 710 confocal microscope. Regrowth length was measured as previously described (Chen et al., 2011 (link); Wu et al., 2007 (link)). To account for day-to-day variability in the regeneration response, regrowth data from mutant animals were normalized to control animals axotomized on the same day. Non-normalized raw values are presented in Table S2.

Andrusiak M.G., Sharifnia P., Lyu X., Wang Z., Dickey A.M., Wu Z., Chisholm A.D, & Jin Y. (2019). Inhibition of axon regeneration by liquid-like TIAR-2 granules. Neuron, 104(2), 290-304.e8.

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