Cfi plan fluor 10 0.30 n a objective lens

Manufactured by Nikon

Sourced in Japan

The CFI plan fluor 10 × /0.30 N.A. objective lens is a high-quality optical component designed for use in various laboratory instruments. It provides a 10× magnification with a numerical aperture of 0.30, enabling efficient light gathering and imaging capabilities. The plan fluor design ensures a flat image field for accurate and consistent observations.

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Lab products found in correlation

Intensilight, by Nikon (4 mentions) Orca r2, by Hamamatsu Photonics (4 mentions) Eclipse ti inverted fluorescence microscope, by Nikon (3 mentions) Gfp hyq, by Nikon (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Nikon (3 mentions) Ti s er motorized stage with encoders, by Nikon (2 mentions) Tritc, by Nikon (1 mentions)

4 protocols using cfi plan fluor 10 0.30 n a objective lens

Inverted Fluorescence Microscopy Protocol

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The sample containing cells was placed on the stage of a Nikon ECLIPSE Ti inverted fluorescence microscope equipped with a CFI plan fluor 10 × /0.30 N.A. objective lens (Nikon, Tokyo, Japan), CCD camera (ORCA-R2; Hamamatsu Photonics, Hamamatsu City, Japan), mercury lamp (Intensilight; Nikon), XYZ automated stage (Ti-S-ER motorized stage with encoders; Nikon), and filter cubes for fluorescence channels (DAPI, GFP HYQ, TRITC; Nikon). For image acquisition, the exposure times were set to 200 ms for DAPI, 200 ms for GFP HYQ (for ALB), 50 ms for GFP HYQ (for CK19), and 200 ms for TRITC (for CYP3A7).

Yoshimoto K., Minier N., Yang J., Imamura S., Stocking K., Patel J., Terada S., Hirai Y, & Kamei K.I. (2020). Recapitulation of Human Embryonic Heartbeat to Promote Differentiation of Hepatic Endoderm to Hepatoblasts. Frontiers in Bioengineering and Biotechnology, 8, 568092.

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Microscopic Analysis of Cell Viability

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The chips were placed on the stage of a Nikon ECLIPSE Ti inverted fluorescence microscope, which was equipped with a CFI plan fluor 10×/0.30 N.A. objective lens (Nikon, Tokyo, Japan), charge-coupled device (CCD) camera (ORCA-R2; Hamamatsu Photonics, Hamamatsu City, Japan), mercury lamp (Intensilight; Nikon), XYZ automated stage (Ti-S-ER motorized stage with encoders; Nikon), and filter cubes for the fluorescence channels (DAPI and GFP HYQ; Nikon). For image acquisition, the exposure times were set to 100 ms for (DAPI) Hoechst 33258, 5 ms for (GFP HYQ) Calcein AM, 2 s for (TRITC) Annexin V, and 100 ms for the (TRITC) AdipoRed assay.

Yang J., Hirai Y., Iida K., Ito S., Trumm M., Terada S., Sakai R., Tsuchiya T., Tabata O, & Kamei K.I. (2023). Integrated-gut-liver-on-a-chip platform as an in vitro human model of non-alcoholic fatty liver disease. Communications Biology, 6, 310.

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Fluorescence Microscopy Imaging Protocol

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Samples containing cells were placed on the imaging stage of a Nikon ECLIPSE Ti inverted fluorescence microscope equipped with a CFI Plan Fluor 10×/0.30 NA objective lens (Nikon), CCD camera (ORCA-R2; Hamamatsu Photonics), mercury lamp (Intensilight; Nikon), XYZ automated stage (Ti-S-ER motorized stage with encoders; Nikon), and filter cubes for the fluorescence channels (DAPI, GFP HYQ, and TRITC; Nikon).

Wen X., Takahashi S., Hatakeyama K, & Kamei K.I. (2021). Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures. Micromachines, 12(5), 550.

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Microscopic imaging of fluorescent cells

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The sample containing cells was placed on the stage of a Nikon ECLIPSE Ti inverted fluorescence microscope equipped with a CFI plan fluor 10×/0.30 N.A. objective lens (Nikon, Tokyo, Japan), CCD camera (ORCA-R2; Hamamatsu Photonics, Hamamatsu City, Japan), mercury lamp (Intensilight; Nikon), XYZ automated stage (Ti-S-ER motorized stage with encoders; Nikon), and filter cubes for fluorescence channels (DAPI and GFP HYQ; Nikon). For image acquisition, the exposure times were set at 500 ms for DAPI and 500 ms for GFP HYQ (for A1AT).

Kamei K.I., Yoshioka M., Terada S., Tokunaga Y, & Chen Y. (2019). Three-dimensional cultured liver-on-a-Chip with mature hepatocyte-like cells derived from human pluripotent stem cells. Biomedical microdevices, 21(3).

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