These two methods have been depicted in detail in our previous study [28 (link)]. The primer sequences are summarized in Table 1 . The primary antibodies in this study included CGRP (ab283568, 1 : 1000, Abcam, USA), CALCRL (861587, Zen Bio, Chengdu, China), RAMP1(385544, Zen Bio, Chengdu, China), Bax (200958, 1 : 1000, Zen Bio, Chengdu, China), Bcl2 (381702, 1 : 1000, Zen Bio, Chengdu, China), Cleaved-caspase3, iNOS (1 : 1000, Zen Bio, Chengdu, China), COX-2 (1 : 1000, Zen Bio, Chengdu, China), MMP3 (384995, 1 : 1000, Zen Bio, Chengdu, China), type I collagen (1 : 1000, ab34710, Abcam), type II collagen (1 : 1000, Zen Bio, Chengdu, China), aggrecan (ab36861, 1 μg/mL, Abcam, USA), NF-κB p65 (D14E12, #8242, Cell Signaling Technology, Inc., three Trask Lane Danvers, United States), p-p65 (Ser536; #3033, Cell Signaling Technology, Inc., three Trask Lane Danvers, United States), Ik-Ba (380682, 35 kDa; Zen Bio, Chengdu, China, 1 : 1,000), p-Ik-Ba (340776, 35 kDa; Zen Bio, Chengdu, China, 1 : 1,000), ERK1/2 (201245-4A4, 42/44 kDa; Zen Bio, Chengdu, China, 1 : 1,000), p-ERK1/2 (301245, 42/44 kDa; Zen Bio, Chengdu, China, 1 : 1,000), p38 (200782, 43 kDa; Zen Bio, Chengdu, China, 1 : 500), p-p38 (310069, 43 kDa; Zen Bio, Chengdu, China, 1 : 1,000), JNK (381100, 46/54 kDa; Zen Bio, Chengdu, China, 1 : 1,000), and p-JNK (380556, 46/54 kDa; Zen Bio, Chengdu, China, 1 : 1,000).
Ab283568
Manufactured by Abcam
Sourced in United States
Ab283568 is a protein-based reagent used in laboratory research. It is a primary antibody that can be used to detect and study specific target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Nf κb p65, by Cell Signaling Technology (1 mentions)
Ramp1, by ZenBio (1 mentions)
Erk1 2, by ZenBio (1 mentions)
P erk1 2, by ZenBio (1 mentions)
Ab34710, by Abcam (1 mentions)
Ab36861, by Abcam (1 mentions)
Bcl2 associated x (bax), by ZenBio (1 mentions)
Gb23303, by Wuhan Servicebio Technology (1 mentions)
Gb21301, by Wuhan Servicebio Technology (1 mentions)
Gb22303, by Wuhan Servicebio Technology (1 mentions)
2 protocols using ab283568
1
Detailed Immunohistochemistry Antibody Protocol
2
Immunohistochemical Analysis of Osteoarthritis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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These two methods have been explained in detail in our previous study [28 (link)]. The primary antibodies included CGRP (ab283568, 1 : 1000, Abcam, USA), CALCRL, RAMP1, type II collagen, Cleaved-caspase-3, iNOS, aggrecan, MMP3, and NF-κB p65. The secondary antibody included HRP-conjugated secondary antibody for goat or mouse (GB23303, Servicebio), Cy3-conjugated Goat Anti-mouse IgG (GB21301, Servicebio), and FITC-conjugated Goat Anti-Rabbit IgG (GB22303, Servicebio).
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