BCBL-1 cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS, Gibco-BRL, Gaithersburg, MD, USA). For the lytic replication of KSHV, BCBL-1 cells were induced with 20 ng/mL 12-O-tetradecanoyl-phorbol-13-acetate (PMA/TPA) (Beyotime Institute of Biotechnology, Wuhan, China). Human iSLK.219 cells, derived from iSLK cells, were latently infected with a recombinant rKSHV.219 virus and also contained a doxycycline-inducible RTA [26 (link)]. The rKSHV.219 expresses the red fluorescent protein (RFP) from the KSHV lytic PAN promoter, the green fluorescent protein (GFP) from the EF-1α promoter [27 (link)]. Human iSLK.219 cells were cultured in DMEM supplemented with 10% FBS. The cells were reactivated with 1 µg/mL doxycycline (Dox) and 1.2 mM sodium butyrate (NaB). The chemicals used for cell culture were purchased from Invivogen (San Diego, CA, USA). Celecoxib was purchased from Sigma-Aldrich (St. Louis, MO, USA), NS-398, SP600125 and SB203580 were purchased from Beyotime Institute of Biotechnology, and nimesulide and cidofovir (CDV) were obtained from Selleck Chemicals (Shanghai, China). All drugs were dissolved in dimethylsulfoxide (DMSO).
Cidofovir cdv
Manufactured by Selleck Chemicals
Sourced in China
Cidofovir (CDV) is a synthetic nucleotide analogue with antiviral properties. It is primarily used as a laboratory reagent for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Nimesulide, by Selleck Chemicals (1 mentions)
Celecoxib, by Merck Group (1 mentions)
Pma tpa, by Beyotime (1 mentions)
Ns 398, by Beyotime (1 mentions)
Sp600125, by Beyotime (1 mentions)
Sb203580, by Beyotime (1 mentions)
Rpmi 1640 hyclone, by Cytiva (1 mentions)
293t cell, by American Type Culture Collection (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Ly2228820, by Selleck Chemicals (1 mentions)
3 protocols using cidofovir cdv
1
KSHV Replication and Inhibition Assay
2
EBV-positive B lymphoma cell lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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B95-8 and P3HR-1 EBV-positive B lymphoma cell lines were kindly provided by Professor Y. Cao at Central South University (Changsha, China). 293T cells were purchased from the American Type Culture Collection. EBV cells were maintained in RPMI-1640 (HyClone; Cytiva) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc.), while 293T cells were maintained in DMEM (HyClone; Cytiva) supplemented with 10% FBS at 37°C with 5% CO2. All cell lines were authenticated via short tandem repeat analysis and cultured for 5 months following resuscitation. TP, cycloheximide (both purchased from Merck KGaA), and cidofovir (CDV; Selleck Chemicals) were dissolved in DMSO.
3
Culturing KSHV+ PEL Cell Line BCBL-1
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