Anti aldh1a1 antibody

Immunofluorescence and immunoblotting were performed as previously described [19 (link)]. The following antibodies were used for immunoblotting. Anti-KMT2D antibody (dilution 1:1000, #ABE1867, Sigma); anti-CD133 antibody (dilution 1:1000, #18,470, Proteintech); anti-ALDH1A1 antibody (dilution 1:2000, #15,910, Proteintech); anti-OCT4 antibody (dilution 1:1000, #11,263, Proteintech); anti-β-catenin antibody (dilution 1:2000, #51,067, Proteintech); anti-c-Myc antibody (dilution 1:100, #ab32072, Abcam); anti-Cyclin D1 antibody (dilution 1:1000, #26,939, Proteintech); anti-H3K4me1 antibody (dilution 1:500, #ab8895, Abcam); and anti-H3K27ac antibody (dilution 1:500, #ab4729, Abcam); anti-MEF2A antibody (dilution 1:500, #12,382, Proteintech). The following antibodies were used for immunofluorescence. Anti-KMT2D: antibody (dilution 1:100, #27,266, Proteintech); anti-CD133 antibody (dilution 1:200, #18,470, Proteintech); anti-β-catenin antibody (dilution 1:200, #51,067, Proteintech).

Protein extraction and western blotting were performed as described previously [46 (link)]. The antibodies were as follows: anti-α-SMA (abcam, ab5694), Vimentin (Proteintech, 10366-1-AP), anti-E-cadherin antibody (proteintech, 20874-1-AP), anti-CD63 antibody (santa cruze, sc-5275), anti-CD81 antibody (proteintech, 27855-1-AP), anti-TSG101 antibody (Santa Cruz, California, United States, sc-7964), anti-RNF43 antibody (bioss, bs-24331R), anti-β-catenin antibody (proteintech, 51067-2-AP), anti-ALDH1A1 antibody (proteintech, 15910-1-AP), anti-OCT4 antibody (proteintech, 11263-1-AP), anti-N-cadherin antibody (proteintech, 66219-1-Ig), and anti-α-tubulin (abcam, ab7291). Protein levels were normalized to α-tubulin and analyzed by Image J software.