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Nexera lc system

Manufactured by AB Sciex
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Sourced in Germany

The Nexera LC system is a liquid chromatography (LC) system designed for high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) applications. It is a modular system that can be configured with various components, such as pumps, autosamplers, column ovens, and detectors, to meet the specific requirements of different analytical workflows.

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Lab products found in correlation

Qtrap 5500 mass spectrometer, by AB Sciex (1 mentions) Biphenyl column, by Phenomenex (1 mentions) Sciex qtrap 5500, by AB Sciex (1 mentions) 6500 qtrap ms, by AB Sciex (1 mentions) Analyst 1, by AB Sciex (1 mentions)

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Nexera system (3 citations)

3 protocols using nexera lc system

1

Quantitative LC-MS/MS Analysis of Compounds

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The DSS were extracted with 3 mL methanol using ultrasonication for 15 minutes. After evaporation (40°C, N2, 100 µL propan-2-ol/HCl [v/v; 3/1]), samples were reconstituted in 100 µL mobile phase and analyzed using an liquid chromatography-electrospray ionization-tandem mass spectrometry system consisting of a Shimadzu Nexera LC system and a Sciex QTRAP 5500 mass spectrometer. Analysis was done in positive scheduled multiple reaction monitoring (sMRM) mode using a Phenomenex biphenyl column for chromatographic separation. More details are given in Supplement 2 .
Hanke K., Fiedler S., Grumann C., Ratmann O., Hauser A., Klink P., Meixenberger K., Altmann B., Zimmermann R., Marcus U., Bremer V., Auwärter V, & Bannert N. (2020). A Recent Human Immunodeficiency Virus Outbreak Among People Who Inject Drugs in Munich, Germany, Is Associated With Consumption of Synthetic Cathinones. Open Forum Infectious Diseases, 7(6), ofaa192.
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2

Amino Acid, Homocysteine, and Enzyme Analysis

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Serum amino acids, homocysteine, and aminotransferases were measured using standard biochemical methods at Children’s Medical Center, University of Texas Southwestern Medical Center. SAH and S-adenosylmethionine (SAM) levels were measured by tandem mass spectrometry (Shimadzu Nexera LC System interfaced with a 5500QTRAP® Sciex) at the Institute for Metabolic Disease at Baylor University Medical Center, Dallas, TX as previously described [18 ].
Stender S., Chakrabarti R.S., Xing C., Gotway G., Cohen J.C, & Hobbs H.H. (2015). Adult-onset liver disease and hepatocellular carcinoma in S-adenosylhomocysteine hydrolase deficiency. Molecular genetics and metabolism, 116(4), 269-274.
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3

Quantitative LC-MS Analysis of Samples

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Analysis of samples was performed on a liquid chromatography-mass spectrometry system (LC-MS) consisting of a Shimadzu Nexera LC-system and a Sciex QTrap 6500 MS controlled by Analyst 1.7 from AB Sciex (Darmstadt, Germany). Compound specific parameters (parent ion, fragment and collision energy) were obtained by automatic tuning using DiscoveryQuant 3.0.7. These parameters were stored in a database to be used for selective quantitation of each test article. Samples (2 µL) were injected onto a Phenomenex Kinetex Polar C18, 2.1 x 30 mm, 2.6 µm column (Brechbühler, Schlieren, Switzerland) and were eluted with a gradient of 0.1 % formic acid in water (mobile phase A) versus 0.1 % formic acid in acetonitrile (mobile phase B) at a flow of 0.8 mL/min at 50°C using the following gradient: 0 min 2 % B; 0.2 min 2 % B; 1 min 60 %B; 1.3 min 100 % B; 1.7 min 100 % B; 1.71 min 2 % B and 1.95 min 2 % B.
Trunzer M., Teigão J., Huth F., Poller B., Desrayaud S., Rodríguez-Pérez R, & Faller B. (2024). Improving In Vitro-In Vivo Extrapolation of Clearance Using Rat Liver Microsomes for Highly Plasma Protein-Bound Molecules. Drug metabolism and disposition: the biological fate of chemicals, 52(5).
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