1-Vinyl imidazole (VIM, ≥98%), N-isopropyl acrylamide (NIPAM, ≥99%), 2,2′-azoisobutyronitrile (AIBN, ≥98%), 1,4-dioxane (DOA, ≥99%), 1, 5-dibromopentane (DMP, ≥97%), polyethyleneimine (PEI, branched, Mw = 10 000, ≥99%), sodium polystyrene sulfonate (PSS, ≥99%), potassium persulfate (≥99%), and N,N′-methylenebis(acrylamide) (MBA, ≥98%) were all purchased at J & K Chemical Co., Ltd. (Beijing, Shanghai). Ascorbic acid (C6H8O6, ≥99%) and potassium antimony tartrate (≥99%) were obtained from Sinopharm Group Chemical Reagent Co., Ltd. Ammonium molybdate tetrahydrate ((NH4)6MO7O24·4H2O, ≥99%) was bought from Shanghai Shenbo Chemical Co., Ltd. Potassium dihydrogen phosphate (KH2PO4, ≥99%) was obtained from Yonghua Chemical Technology Co., Ltd. (Suzhou, China). Sodium phosphate dodecahydrate (Na3PO4·12H2O, ≥99%) was purchased from Jiangsu Qiangsheng Chemical Co., Ltd. (Changshu, China).
N isopropylacrylamide
Manufactured by J&K Scientific
Sourced in China
N-Isopropylacrylamide is a chemical compound used in various laboratory applications. It is a white, crystalline solid with the chemical formula C6H11NO. The compound exhibits temperature-responsive properties, making it useful in the development of stimuli-responsive materials and systems.
Automatically generated - may contain errors
Lab products found in correlation
N n methylenebisacrylamide, by J&K Scientific (3 mentions)
Potassium persulfate, by J&K Scientific (2 mentions)
2 2 azoisobutyronitrile, by J&K Scientific (1 mentions)
1 4 dioxane, by J&K Scientific (1 mentions)
Ascorbic acid, by Sinopharm (1 mentions)
Potassium antimony tartrate, by Sinopharm (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Acrylic acid, by Merck Group (1 mentions)
Rpmi 1640 medium, by Cytiva (1 mentions)
Dulbecco modified eagle medium (dmem), by Cytiva (1 mentions)
Hydrogen peroxide, by J&K Scientific (1 mentions)
3 bromo 1 propanol, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Tetraethoxysilane, by Shanghai Lingfeng Chemical Reagent (1 mentions)
Sodium phosphate tribasic dodecahydrate, by Sinopharm (1 mentions)
Milli q system, by Merck Group (1 mentions)
Methanol, by Sinopharm (1 mentions)
Hydrochloric acid (hcl), by Sinopharm (1 mentions)
Potassium dihydrogen phosphate, by Sinopharm (1 mentions)
Sodium dodecyl sulfate (sds), by J&K Scientific (1 mentions)
6 protocols using n isopropylacrylamide
1
Synthesis of Polymeric Materials
2
Synthesis and Characterization of Doxorubicin-loaded Hydrogel Nanoparticles
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Acrylic acid (Sigma-Aldrich, China) was distilled before use. N,N’-methylenebisacrylamide and N-Isopropylacrylamide (J&K Scientific., Ltd., Shanghai, China) were recrystallized from methanol and n-hexane, respectively. Doxorubicin hydrochloride (DOX HCl) was purchased from Xianghe Shunda Fine Chemical Co., Ltd. (Wuhan, China). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC.HCl), N-Hydroxysuccinimide (NHS), potassium persulfate, sodium dodecylsulfate, and 5-aminofluorescein were analytic grade and purchased from J&K Technology, Ltd. cRGD was synthesized by ABBiochem Co., Ltd. (Shanghai, China). DMEM and RPMI-1640medium were obtained from HyClone Laboratories, Inc. (Utah, USA). Trypsin was purchased from Gibco-BRL Life Technologies (Carlsbad, CA). Biosharp (Hefei, China) supplied DAPI and MTT. All other chemicals were analytical grade and used without further treatment.
3
Synthesis of Functional Polymeric Materials
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Sodium borohydride and 3-bromo-1-propanol were purchased from Sigma-Aldrich. Acrylic chloride and tellurium powder were purchased from Shanghai Reagent Company. Acrylic chloride was purchased from Anhui Wotu Co., Ltd. and used after being purified by vacuum distillation. N,N′-Methylene diacrylamide (MBA), acrylamide (AM), hexadecyl trimethyl ammonium bromide (CTAB), 5,5′-dithio-bis(2-nitrobenzoic acid), 4-nitrothiophenol (NBT), cumyl hydroperoxide, and hydrogen peroxide were used as received from J&K Scientific Ltd. N-Isopropylacrylamide (NIPAM) was purchased from J&K Scientific Ltd., and used after cyclohexane/methanol recrystallization and drying. 2-Bromopropionyl bromide was purchased from Lancaster. Tris(2-dimethylaminoethyl)amine (ME6TREN) was prepared according to the previously reported methods.51 (link) Triethylamine and tetrahydrofuran were purchased from Nanning Lantian Reagent Co., Ltd., and used after rigorously dried with sodium. 3-Carboxyl-4-nitrobenzenethiol (TNB) was synthesized from 5,5′-dithiobis(2-nitrobenzoic acid) using the previously reported methods.20 (link)
4
Synthesis of Functional Polymer Nanoparticles
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N-isopropyl acrylamide (NIPAM, 99%), 3-(methoxymethylsilyl) propyl methacrylate (TMSPMA 98%), and potassium persulfate (KPS 99%) were bought from J&K Chemical Ltd. (Beijing, China) and used as received; analytical grade tetraethoxysilane (TEOS, ≥28%, SiO2) was bought from Shanghai Lingfeng Chemical Reagent (Shanghai China); 3-ureidopropyltriethoxysilane (UPTES, 40.0–50.0%, methanol solution) was obtained from Aladdin Reagent (Shanghai, China). N-Cetyltrimethylammonium bromide (CTAB, 99%) was purchased from Shanghai Richjoint Chemical Reagents (Shanghai, China). Sodium phosphate tribasic dodecahydrate, potassium dihydrogen phosphate, methanol, sodium hydroxide (NaOH), ascorbic acid, antimony potassium tartrate, and hydrochloric acid (HCl) were of analytical grade and were purchased from Sinopharm Chemical Reagent (Beijing, China); Ammonium molybdate tetrahydrate (99%) was purchased from Shanghai Shenbo Chemical (Shanghai, China). Deionized water, which was purified by a Millipore Milli-Q system, was put to use in all experiments.
5
Synthesis of NIPAM-based Hydrogels
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Monomers N-isopropylacrylamide (NIPAM), Methyl methacrylate (MAA), the crosslinker N,N-methylenebisacrylamide, surfactants sodium dodecyl sulfate (SDS), and initiator potassium persulfate were purchased from J & K Scientific Ltd., Beijing, China. Ammonia water (NH3∙H2O) was purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. All other chemicals and solvents used in this paper were of analytical grade and solutions were prepared from distilled water.
6
Silica Wafer Surface Modification
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Silica
wafers (2.0 cm × 2.0 cm) were
treated in a mixture of H2SO4 and 30% H2O2 (volumetric ratio = 7:3) for 30 min, rinsed
with deionized water, and
dried under nitrogen gas (N2) flow. The N-Isopropylacrylamide (NIPAAm) monomer was purchased from J&K
Chemical, N,N,N′,N″,N″-pentamethyldiethylenetriamine
(PMDETA) was purchased from TCI. Trichloro(octyl)silane (TCOS), octadecyltrichlorosilane
(OTCS), 3-aminopropyl trimethoxysilane (APTMS), 3-mercaptopropyl trimethoxysilane
(MPTMS), trichloro(1H,1H,2H,2H-perfluorooctyl) silane (PFS), 2-bromoisobutyryl
bromide, and copper(I) chloride (CuCl) were provided by Aldrich. The
polystyrene microparticles with a diameter of 620 nm were provided
by Wuhan Sphere Scientific Co., Ltd. and were cleaned with water and
50% ethanol several times before use. Dichloromethane, absolute ethanol,
triethylamine, sodium dodecyl sulfate (SDS), methanol, 1,2-dichloroethane,
and the four components of the photoresist were used directly after
received. The masks employed for photolithography were machined by
the Institute of Microelectronics of Chinese Academy of Sciences.
The TJ-1A-Micro Flow syringe pump was purchased from Baoding Longer
Precision Pump Co., Ltd. The water in all experiments was deionized
and doubly distilled prior to use.
wafers (2.0 cm × 2.0 cm) were
treated in a mixture of H2SO4 and 30% H2O2 (volumetric ratio = 7:3) for 30 min, rinsed
with deionized water, and
dried under nitrogen gas (N2) flow. The N-Isopropylacrylamide (NIPAAm) monomer was purchased from J&K
Chemical, N,N,N′,N″,N″-pentamethyldiethylenetriamine
(PMDETA) was purchased from TCI. Trichloro(octyl)silane (TCOS), octadecyltrichlorosilane
(OTCS), 3-aminopropyl trimethoxysilane (APTMS), 3-mercaptopropyl trimethoxysilane
(MPTMS), trichloro(1H,1H,2H,2H-perfluorooctyl) silane (PFS), 2-bromoisobutyryl
bromide, and copper(I) chloride (CuCl) were provided by Aldrich. The
polystyrene microparticles with a diameter of 620 nm were provided
by Wuhan Sphere Scientific Co., Ltd. and were cleaned with water and
50% ethanol several times before use. Dichloromethane, absolute ethanol,
triethylamine, sodium dodecyl sulfate (SDS), methanol, 1,2-dichloroethane,
and the four components of the photoresist were used directly after
received. The masks employed for photolithography were machined by
the Institute of Microelectronics of Chinese Academy of Sciences.
The TJ-1A-Micro Flow syringe pump was purchased from Baoding Longer
Precision Pump Co., Ltd. The water in all experiments was deionized
and doubly distilled prior to use.
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