Sporophylls of the brown algae U. pinnatifida were obtained from the seaweed-processing factory in Wando, South of Korea. Impurities were removed using tap water, and the samples were subjected to freeze-drying and stored at −40 °C. Trifluoroacetic acid (TFA) of analytical grade (Dae-Jung Chemicals & Metals Co., Seoul, Korea) was used for the monosaccharide compositional analysis and 97% ethanol (Dae-Jung Chemicals & Metals Co., Seoul, Korea) was used for extraction. Celluclast was purchased from Novo Nordisk (Bagsvaerd, Denmark). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), and AAPH were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents used in these experiments were of analytical grade.
Trifluoroacetic acid tfa
Manufactured by Daejung Chemicals
Trifluoroacetic acid (TFA) is a colorless, corrosive liquid that is commonly used in analytical and organic chemistry laboratories. It is a strong acid with a pungent odor. TFA is a versatile reagent that can be used for various applications, including peptide synthesis, protein purification, and organic synthesis.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dimethyl sulfoxide dmso, by Merck Group (1 mentions)
Celluclast, by Novo Nordisk (1 mentions)
Methyl sulfonic acid, by Merck Group (1 mentions)
Ginsenoside rg3, by Merck Group (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Piperidine, by Daejung Chemicals (1 mentions)
Diethyl ether, by Daejung Chemicals (1 mentions)
N n dimethylformamide (dmf), by Daejung Chemicals (1 mentions)
Methanol, by Daejung Chemicals (1 mentions)
Dichloromethane dcm, by Daejung Chemicals (1 mentions)
6 protocols using trifluoroacetic acid tfa
1
Monosaccharide Analysis of Sporophylls from Undaria pinnatifida
2
Chemical Reagents Procurement Protocol
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Bisphenol A, terphenyl (TP), biphenyl (BP), dibenzyl (DB), trifluorosulfonic acid (TFSA), and N‐methyl piperidine (MP) were purchased from TCI Development Co., Ltd. Methylsulfonic acid was purchased from Sigma‐Aldrich Chemical Co. (Milwaukee, WI, USA). Trifluoroacetic acid (TFA) and other chemicals were purchased from Daejung Chemicals & Metals (Siheung‐si, Gyeonggi‐do, Korea). All chemicals were used without further purification.
3
Phytochemical Profiling of Herbal Extracts
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Naringin, hesperidin, glycyrrhizin, atractylenolide III, and magnolol were provided by Korea Food and Drug Administration. The purities of these compounds were determined to be higher than 98% by HPLC and stored at 4C. HPLC grade acetonitrile, methanol and water were purchased from J. K. Baker (USA). Trifluoroacetic acid (TFA) was purchased from DAE JUNG (Korea). PWS samples were provided by Korea Institute of Oriental Medicine.
4
Analytical Characterization of Medicinal Herbs
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Ginsenoside Rg3 and caffeic acid were purchased from Sigma (USA). Puerarin, hesperidin, naringin, glycyrrhizin, and 6-gingerol were purchased from the Korea Food and Drug Administration. Costunolide was purchased from Chromadex (USA). The purity of each compound was determined to be above 98%. The chemical structures of the eight marker compounds were shown in Figure 1 . Acetonitrile and water were of HPLC grade and purchased from J.T. Baker (USA). Analytical grade trifluoroacetic acid (TFA) was obtained from DAE JUNG (Korea). Samsoeum sample was prepared, and other commercial Samsoeum samples were obtained from different herbal medicine companies.
5
Isolation and Identification of Phytosterols
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Campesterol, stigmasterol and daucosterol used for standard compounds were isolated from A. apiacea by silica gel column chromatography. Structures of isolated three compounds were determined by spectroscopic methods, including nuclear magnetic resonance spectrum and compared with spectroscopic data of the literatures.
High performance liquid chromatography-grade acetonitrile (ACN) and water were purchased from J. T. Baker (USA). Trifluoroacetic acid (TFA) was purchased from DAE JUNG (Korea). Methanol and dimethyl sulfoxide (DMSO) was purchased from DAE JUNG (Korea).
High performance liquid chromatography-grade acetonitrile (ACN) and water were purchased from J. T. Baker (USA). Trifluoroacetic acid (TFA) was purchased from DAE JUNG (Korea). Methanol and dimethyl sulfoxide (DMSO) was purchased from DAE JUNG (Korea).
6
Solid-Phase Peptide Synthesis Protocol
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2-Chlorotrityl chloride
(CTC) (100–200 mesh, 1.34 mmol/g) resin, fritted polypropylene
tubes (Libra tube RT-20M, 20 mL), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium
hexafluorophosphate (HBTU), hydroxybenzotriazole (HOBt), and Fmoc-protected
amino acids were purchased from BeadTech Inc. (Seoul, Korea). N,N-Diisopropylethylamine (DIPEA), phenol,
thioanisole, and 1,2-ethanedithiol (EDT) were purchased from Aldrich
(St. Louis, MO, USA). Dichloromethane (DCM), N,N-dimethylformamide (DMF), methanol, piperidine, diethyl
ether, and trifluoroacetic acid (TFA) were obtained from Dae-Jung
Chemicals (Korea). Hydrogen peroxide solution (30%), sulfuric acid
(98%), DMSO, and CuCl2 were of analytical grade and used
as purchased.
(CTC) (100–200 mesh, 1.34 mmol/g) resin, fritted polypropylene
tubes (Libra tube RT-20M, 20 mL), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium
hexafluorophosphate (HBTU), hydroxybenzotriazole (HOBt), and Fmoc-protected
amino acids were purchased from BeadTech Inc. (Seoul, Korea). N,N-Diisopropylethylamine (DIPEA), phenol,
thioanisole, and 1,2-ethanedithiol (EDT) were purchased from Aldrich
(St. Louis, MO, USA). Dichloromethane (DCM), N,N-dimethylformamide (DMF), methanol, piperidine, diethyl
ether, and trifluoroacetic acid (TFA) were obtained from Dae-Jung
Chemicals (Korea). Hydrogen peroxide solution (30%), sulfuric acid
(98%), DMSO, and CuCl2 were of analytical grade and used
as purchased.
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