8 well chambered slides

Manufactured by Thermo Fisher Scientific

Sourced in United States

The 8-well chambered slides are a laboratory equipment designed to facilitate cell culture and microscopy applications. The product provides eight individual chambers on a single slide, allowing for the simultaneous culturing and observation of multiple samples or cell types. The chambers are transparent and made of a material suitable for live-cell imaging and analysis.

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Lab products found in correlation

Lsm 780 confocal microscope, by Zeiss (3 mentions) Axiovert 100 tv epifluorescence microscope, by Zeiss (1 mentions) Mitotracker green fm, by Thermo Fisher Scientific (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Vectashield antifade mounting medium with dapi, by Vector Laboratories (1 mentions) Alexa fluor 488 or 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Lsm 880 confocal system, by Zeiss (1 mentions) Alexa fluor 488 conjugated goat anti mouse igg h l antibody, by Abcam (1 mentions) Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Bz x800 fluorescence microscope, by Keyence (1 mentions)

Close spelling variants of this product

8-well chambered cover glass slides Falcon 8-well chambered cell culture slides 8-well chambered glass slides Chambered slides

10 protocols using 8 well chambered slides

Fluorescent probe cellular uptake

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The A549, OVCAR-4, and HT-1080 cells were seeded into 8-well chambered slides (Lab-Tek, Nunc, USA) and were grown to 70% confluency. Cells were incubated with 1 μM probe (SF8(DRADG)₂, SF8(DDGRG)₂, or SF8(DRGDG)₂) in media for 30 min at 37 °C. The cells were washed three times with phosphate buffered saline and fixed with 4% cold paraformaldehyde for 20 min at room temperature. Cells were then washed one time with phosphate buffered saline, co-stained with 3 μM Hoechst 33342 for 10 min at room temperature, washed two times with phosphate buffered saline, and imaged.

Schreiber C.L., Zhai C, & Smith B.D. (2021). Structural Engineering of Fluorescent Self-Threaded Peptide Probes for Targeted Cell Imaging. Photochemistry and photobiology, 98(2), 354-361.

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Cell-based Probe Uptake Assay

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The A549, OVCAR-4, and HT-1080 cells were seeded into 8-well chambered slides (Lab-Tek, Nunc, USA) and were grown to 70% confluency. Prior to probe incubation, the cells were placed either in the fridge (4 °C) or a cell incubator (37 °C) for 30 min. The cells were then washed with phosphate buffered saline and incubated at the same temperature as before with 1 μM SF8(DRGDG)₂ in media for 30 min. The cells were washed three times with phosphate buffered saline, co-stained with 3 μM Hoechst 33342 for 10 min at room temperature, washed two times with phosphate buffered saline, and imaged.

Schreiber C.L., Zhai C, & Smith B.D. (2021). Structural Engineering of Fluorescent Self-Threaded Peptide Probes for Targeted Cell Imaging. Photochemistry and photobiology, 98(2), 354-361.

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Visualizing Integrin-Mediated Cell Adhesion

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The A549 cells were seeded into 8-well chambered slides (Lab-Tek, Nunc, USA) and were grown to 70% confluency. Cells were co-incubated with 1 μM Cy3-cRGDfK (Molecular Targeting Technologies Inc.) and 1 μM SF8(DRGDG)₂ in media for 30 min at 37 °C. The live cells were then washed three times with phosphate buffered saline, co-stained with 3 μM Hoechst 33342 for 10 min at room temperature, washed two times with phosphate buffered saline, and imaged.

Schreiber C.L., Zhai C, & Smith B.D. (2021). Structural Engineering of Fluorescent Self-Threaded Peptide Probes for Targeted Cell Imaging. Photochemistry and photobiology, 98(2), 354-361.

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Fluorescent Imaging of Organelle Dynamics

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HT-1080 cells were seeded into 8-well chambered slides (Lab-Tek, Nunc, USA) and were grown to 70% confluency. The cells were incubated with 1 μM SF8 probe in media for 30 min at 37 °C. The cells were washed three times with phosphate buffered saline and co-stained with either 100 nM MitoTracker Green FM (Thermo Fisher Scientific) or 100 nM LysoTracker Red DND-99 (Thermo Fisher Scientific) for 15 min in opti-MEM at 37 °C. Cells were then washed two times with phosphate buffered saline and incubated with 3 μM Hoechst 33342 for 10 min at room temperature. The live cells were washed two additional times and imaged on a Zeiss Axiovert 100 TV epifluorescence microscope equipped with a UV filter (ex: 387/11, em: 447/60), FITC filter (ex: 485/20, em: 524/24), TxRed filter (ex: 562/40, em: 624/40), and Cy5.5 filter (ex: 655/40, em: 716/40). Image processing for each micrograph was then conducted using ImageJ2 software with a 10-pixel rolling ball radius background subtraction.

Schreiber C.L., Zhai C, & Smith B.D. (2021). Chiral figure-eight molecular scaffold for fluorescent probe development. Organic & biomolecular chemistry, 19(14), 3213-3219.

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OVCAR3 Cells RPS6 and PAR Immunostaining

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OVCAR3 cells were seeded on 8-well chambered slides (Thermo Fisher, 154534) one day prior to the experiment. The cells were washed twice with PBS, fixed in 4% paraformaldehyde for 15 minutes at room temperature, and washed three times with PBS. The cells were permeabilized for 5 minutes using Permeabilization Buffer (PBS containing 0.01% Triton X-100), washed three times with PBS, and incubated for 1 hour at room temperature in Blocking Solution (PBS containing 1% BSA, 10% FBS, 0.3M Glycine and 0.1% Tween-20). The fixed cells were incubated with a mixture of RPS6 antibody at a 1:200 dilution and the MAR or PAR detection reagents at a concentration of 20 μg/mL in PBS overnight at 4°C, followed by three washes with PBS. The cells were then incubated with a mixture of Alexa Fluor 594 donkey anti-rabbit IgG (ThermoFisher, A-21207) and Alexa Fluor 488 goat anti-mouse IgG (ThermoFisher, A-11001) each at a 1:500 dilution in PBS for 1 hour at room temperature. After incubation, the cells were washed three times with PBS. Finally, coverslips were placed on cells coated with VectaShield Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200) and images were acquired using an inverted Zeiss LSM 780 confocal microscope.

Challa S., Khulpateea B.R., Nandu T., Camacho C.V., Ryu K.W., Chen H., Peng Y., Lea J.S, & Lee Kraus W. (2021). Ribosome ADP-Ribosylation Inhibits Translation and Maintains Proteostasis in Cancers. Cell, 184(17), 4531-4546.e26.

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Osteoclastogenesis Assay with RANKL

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RAW264.7 (3 × 10³ cells/well) and BMMs (5 × 10³ cells/well) were seeded in 96‐well plates and stimulated with RANKL (50 ng/mL) for 5 days. Cells were subjected to TRAP staining as described.⁽²¹⁾ TRAP‐positive multinucleated cells (≥3 nuclei) were scored as OC. For F‐actin staining, BMMs were seeded in 8‐well chambered slides (Thermo Fisher Scientific) at the density of 10⁴ cells per well and stimulated with RANKL for 5 days. BMMs were precultured in α‐MEM containing 30 ng mL M‐CSF and 50 ng mL of RANKL for 3 days, then the cells were regarded as mature OCs (mOCs). To determine F‐actin ring formation of mOCs from Wt or Mpo^−/− BMMs, an equal number of mOCs (10⁴ cells/well) were further seeded in 8‐well chambered slides and stimulated with RANKL (50 ng/mL) for another 5 days. Cells were fixed and stained with TRITC‐phalloidin working solution (red) (Yeasen, Shanghai, China) as described previously.⁽²²⁾ Five random fields per sample were counted and averaged for each sample. Each experiment was performed in triplicate and repeated independently more than three times.

Zhao X., Lin S., Li H., Si S, & Wang Z. (2020). Myeloperoxidase Controls Bone Turnover by Suppressing Osteoclast Differentiation Through Modulating Reactive Oxygen Species Level. Journal of Bone and Mineral Research, 36(3), 591-603.

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Immunofluorescence Staining of Cultured Cells

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Cells were seeded into 8-well chambered slides (Thermo Fisher Scientific). After treatment, cells were fixed with 4% paraformaldehyde at 37 °C for 30 min, permeabilized with 0.2% Triton X-100 and incubated in 3% BSA blocking solution for 30 min to block non-specific bindings of antibody. Cells were then incubated with a primary antibody diluted in 3% BSA in PBS overnight at 4 °C. Then, the cells were washed with PBS for 5 min 3 times and incubated with Alexa Fluor 488- or 594-conjugated-secondary antibodies (Thermo fisher Scientific) for 1 h at room temperature in the dark. Cell nuclei were stained with 1 μg/ml Hoechst33342 for 30 min. The fluorescence images were acquired by Zeiss LSM 880 Confocal system (Carl Zeiss, Thornwood, NY).

Ren G., Chen J., Pu Y., Yang E.J., Tao S., Mou P.K., Chen L.J., Zhu W., Chan K.L., Luo G., Deng C, & Shim J.S. (2024). BET inhibition induces synthetic lethality in PTEN deficient colorectal cancers via dual action on p21CIP1/WAF1. International Journal of Biological Sciences, 20(6), 1978-1991.

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Immunofluorescence Assay for iSGECs

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Untreated iSGECs were cultured on 8-well chambered slides (ThermoFisher) coated with gelatin (0.2%) for 24–48 h prior to fixation with 4% PFA. Dilutions for primary and secondary antibodies are listed in Supplementary Table 2. Detailed IF methods are described in Supplementary Methods.

Noll B., Mougeot F.B., Brennan M.T, & Mougeot J.L. (2022). Regulation of MMP9 transcription by ETS1 in immortalized salivary gland epithelial cells of patients with salivary hypofunction and primary Sjögren’s syndrome. Scientific Reports, 12, 14552.

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Cellular Uptake of PEGylated MSbNSs/DOX

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The cellular uptake of the PEGylated MSbNSs/DOX was observed using a confocal laser scanning microscope. In brief, panc 02 cells were seeded into 8-well chambered slides (Thermo Scientific, USA) and cultured for 12 h. Then, the cells were incubated with PEGylated MSbNSs/DOX for 3 h, and followed with Lyso-Tracker (green color) and Hoechst 33342 (blue color) double staining. The cells were washed with PBS for three times and analyzed via confocal laser scanning microscope.

Chen Y., Yu Z., Zheng K., Ren Y., Wang M., Wu Q., Zhou F., Liu C., Liu L., Song J, & Qu J. (2022). Degradable mesoporous semimetal antimony nanospheres for near-infrared II multimodal theranostics. Nature Communications, 13, 539.

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Immunocytochemistry of dsRNA in Cells

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For immunocytochemical analysis, the cells were cultured in 8-well chambered slides (Thermo Scientific, #154534) and treated as previously described. After three washes with PBS, cells were fixed with 4% formaldehyde for 20 min at RT. After two washes cells were incubated with a blocking-permeabilization buffer (5% goat serum and 0.3% Triton X-100 in PBS) for 1 h. Then cells were incubated with anti-dsRNA (J2) antibody diluted (2.5 μg/mL) in antibody diluent buffer (1% BSA in PBS) overnight at 4 °C in a humidified chamber. After three 5 min washes with PBS, the cells were incubated with Alexa Fluor 488 conjugated goat anti-mouse IgG H&L antibody (2 μg/mL) (abcam, #ab150113) at RT for 1 h in the dark. After three 5 min washes, the cells were mounted using the ProLong™ Gold Antifade Mountant with DAPI (Invitrogen, #P36935) and coverslips. Slides were imaged using a KEYENCE BZ-X800 fluorescence microscope.

Wang Y., Pattarayan D., Huang H., Zhao Y., Li S., Wang Y., Zhang M., Li S, & Yang D. (2024). Systematic investigation of chemo-immunotherapy synergism to shift anti-PD-1 resistance in cancer. Nature Communications, 15, 3178.

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