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Lps e coli 0111 b4 lipopolysaccharide

Manufactured by Merck Group

LPS [E.coli 0111:B4 Lipopolysaccharide] is a biochemical compound derived from the outer membrane of Escherichia coli bacteria. It is a complex molecule consisting of a lipid and a polysaccharide component. This product is intended for research use only.

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2 protocols using lps e coli 0111 b4 lipopolysaccharide

1

Dendritic Cell Activation Assay

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Bone marrow derived DCs were generated by flushing out femora and tibiae. Viable cells were cultured in clone medium supplemented with GM-CSF and IL-4 (both 20 ng/ml, R&D Systems) for 6 days with splitting every other day. DCs were removed by vigorous pipetting and cultured in the presence of TLR ligands CpG [ODN 1826] from InVivoGen or LPS [E.coli 0111:B4 Lipopolysaccharide], Sigma-Aldrich.
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2

Dendritic Cell Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow derived DCs were generated by flushing out femora and tibiae. Viable cells were cultured in clone medium supplemented with GM-CSF and IL-4 (both 20 ng/ml, R&D Systems) for 6 days with splitting every other day. DCs were removed by vigorous pipetting and cultured in the presence of TLR ligands CpG [ODN 1826] from InVivoGen or LPS [E.coli 0111:B4 Lipopolysaccharide], Sigma-Aldrich.
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