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Superlumia ecl hrp substrate kit

Manufactured by Merck Group
Sourced in Japan, United States

The SuperLumia ECL HRP Substrate Kit is a laboratory reagent designed to detect and quantify the presence of horseradish peroxidase (HRP) in Western blot and other immunoassay applications. The kit provides a chemiluminescent substrate solution that produces a luminescent signal upon reaction with HRP, which can then be measured to determine the amount of target protein present in the sample.

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2 protocols using superlumia ecl hrp substrate kit

1

Western Blot Analysis of Lipid Metabolism

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Total cellular protein was extracted using RIPA lysis buffer containing protease inhibitor cocktail (Beyotime, China). The extracted proteins were separated by SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, NY), which were subsequently blocked with a 5% solution of nonfat milk for 1 h. The membranes were then incubated with primary antibodies at 4°C overnight, followed by secondary antibody incubation for 1 h at room temperature. The proteins were visualized with a SuperLumia ECL HRP Substrate Kit (Millipore, NY) and detected using an LAS4000 chemiluminescence detection system (Fuji, Tokyo, Japan). The primary antibodies used were as follows: anti-β-actin (1:1,000, AF5001, Beyotime), SREBP1 (1:1,000, 14088-1-AP, Proteintech), and SCD1 (1:500, sc-515844, Santa Cruz Biotechnology). The anti-β-actin antibody was used as an internal control.
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2

Western Blot Analysis of E2F8

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Total cellular protein was extracted using RIPA lysis buffer containing protease inhibitor cocktail (Beyotin, China). The extracted proteins were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, NY, USA), which were subsequently blocked with a 5% solution of skim milk for 1 h. The membranes were then incubated with primary antibodies at 4 °C overnight, followed by secondary antibody incubation for 1 h at room temperature. The proteins were visualized with a SuperLumia ECL HRP Substrate Kit (Millipore, NY, USA) and detected using an LAS4000 chemiluminescence detection system (Fuji, Tokyo, Japan). The primary antibodies used were as follows: anti-β-actin (1:1000, Beyotin, AA128) and anti-E2F8 (1: 500, sc-514064, Santa-Cruz Technology). An anti-β-actin antibody was used as an internal control.
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