Log In Sign up
The largest database of trusted experimental protocols

Sc 5279

Manufactured by Fortis Life Sciences
Visit Supplier

The Sc-5279 is a laboratory equipment product designed for scientific research applications. It serves as a tool for researchers to conduct various experiments and analyses. The core function of the Sc-5279 is to provide a reliable and consistent platform for data collection and analysis, without any further interpretation or extrapolation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Ab13840, by Abcam (2 mentions) Ab9110, by Abcam (2 mentions) Anti suz12, by Cell Signaling Technology (2 mentions) Anti ring1b, by Fortis Life Sciences (2 mentions) Anti cbfa2t2, by Fortis Life Sciences (2 mentions) Alexa fluor 660 streptavidin, by Thermo Fisher Scientific (2 mentions) Streptavidin hrp, by BioLegend (2 mentions) Anti oct4, by Santa Cruz Biotechnology (2 mentions)

2 protocols using sc 5279

1

Purification and Characterization of PRDM14 Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human PRDM14 antigen (N-ter residue 1-243) was generated by using PreScission protease to cleave the recombinant fusion protein GST-PRDM14(1-243). Rabbit polyclonal antibody was affinity purified by affi-gel 15 matrix conjugated with a his6 tagged antigen of PRDM14(1-243). Mouse PRDM14 antibody was described previously29 . Briefly, N-ter construct (amino acids 1-231) of mouse Prdm14 was cloned into pET30a vector (Novagen, #69909-3). The corresponding His6 tagged protein was overexpressed and purified for rabbit polyclonal antigen production. The antibody was purified with Affi-gel 15 as mentioned above. In-house rabbit Ezh2 antibody was reported previously30 . Other antibodies used in this study were from commercial sources with the following catalog numbers: Anti-CBFA2T2: Bethyl A303-593A, anti-OCT4: Santa Cruz #sc-5279, anti-Ring1B: Bethyl #A302-869A, anti-SUZ12: Cell Signaling #3737S, anti-HA: Abcam #ab9110, anti-tubulin Abcam #ab6046, anti-TRA-1-81 biotin: eBiosceince, #13-8883-82, Alexa Fluor 660 Streptavidin: LifeTechnologies s21377, HRP Streptavidin: Biolegend #405210, MVH: Abcam #ab13840.
Tu S., Narendra V., Yamaji M., Vidal S.E., Rojas L.A., Wang X., Kim S.Y., Garcia B.A., Tuschl T., Stadtfeld M, & Reinberg D. (2016). CO-REPRESSOR CBFA2T2 REGULATES PLURIPOTENCY AND GERMLINE DEVELOPMENT. Nature, 534(7607), 387-390.
+ Open protocol
+ Expand
2

Purification and Characterization of PRDM14 Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human PRDM14 antigen (N-ter residue 1-243) was generated by using PreScission protease to cleave the recombinant fusion protein GST-PRDM14(1-243). Rabbit polyclonal antibody was affinity purified by affi-gel 15 matrix conjugated with a his6 tagged antigen of PRDM14(1-243). Mouse PRDM14 antibody was described previously29 . Briefly, N-ter construct (amino acids 1-231) of mouse Prdm14 was cloned into pET30a vector (Novagen, #69909-3). The corresponding His6 tagged protein was overexpressed and purified for rabbit polyclonal antigen production. The antibody was purified with Affi-gel 15 as mentioned above. In-house rabbit Ezh2 antibody was reported previously30 . Other antibodies used in this study were from commercial sources with the following catalog numbers: Anti-CBFA2T2: Bethyl A303-593A, anti-OCT4: Santa Cruz #sc-5279, anti-Ring1B: Bethyl #A302-869A, anti-SUZ12: Cell Signaling #3737S, anti-HA: Abcam #ab9110, anti-tubulin Abcam #ab6046, anti-TRA-1-81 biotin: eBiosceince, #13-8883-82, Alexa Fluor 660 Streptavidin: LifeTechnologies s21377, HRP Streptavidin: Biolegend #405210, MVH: Abcam #ab13840.
Tu S., Narendra V., Yamaji M., Vidal S.E., Rojas L.A., Wang X., Kim S.Y., Garcia B.A., Tuschl T., Stadtfeld M, & Reinberg D. (2016). CO-REPRESSOR CBFA2T2 REGULATES PLURIPOTENCY AND GERMLINE DEVELOPMENT. Nature, 534(7607), 387-390.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!