2 naphtol

The following chemicals were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France): bisphenol-A (BPA purity >99%), triclosan (TCS >97%), benzophenone-3 (BP3_98%), decabromodiphenyl ether (BDE-209 98%), sodium perchlorate (>98%), 4-4′-dichlorodiphenyldichloroethylen (4,4′-DDE 99%), hexachlorobenzene (HCB 99.9%), dibutylphtalate (DBP 99%), diethylhexylphtalate (DEHP 99%), PCB-153, 2-Naphtol (99%), perfluorooctanesulfonic acid (PFOS >98%), perfluorooctanoic acid (PFOA >98%), methyl mercury chloride (>99.9%), lead chloride (98%), dimethyl sulfoxide (DMSO), acetone, 3,3′,5,5′tetraiodo-L-thyronine T₄ >98%) and triiodothyronine (T₃ 99%). NH-3, a thyroid hormone antagonist18 (link) was synthesized by AGV Discovery (France), absence of contamination by benzofurane was verified56 (link). All chemicals were dissolved at 10⁻¹ M in DMSO, with the exception of HCB (10⁻¹ M in acetone) and BDE 209 (10⁻²M in DMSO). These solutions were aliquoted and stored at −20 °C until use. T₃ was prepared in 30% NaOH, 70% milliQ Water at 10⁻²M concentration, aliquoted and stored at −20 °C until use. The chemical mixture was prepared at 10⁵-fold concentration by mixing appropriate volumes of stocks as described in Supplementary Table 1, aliquoted and stored at −20 °C.

Mefenamic acid (≥99%), tacrine (≥99%), carvedilol (≥98%), nifedipine (≥98%), ellipticine, α-naphthoflavone (≥97%), ticlopidine (≥99%), 1-naphtol (≥99%), 2-naphtol (≥98%), 4-methoxy-benzaldehyde (≥98%), 2-(p-tolyl)ethylamine (≥97%) were purchased from Sigma-Aldrich (Schnelldorf, Germany); phenacetin was obtained from Brocades-ACF (Maarssen, the Netherlands). 7-Methoxyresorufin was synthesized by the method of Burke and Mayer [28 (link)] and final purity was assessed to be higher than 95%.