The protein suspension of liver tissues for Western blotting was obtained using a total protein extraction kit (APPLYGEN P1250, Beijing, China) and protein concentrations were determined by BCA protein assay kit following the manufacturer’s instructions. CD68 (ab283654, Abcam), HSP90 (ab59459, Abcam), HSF1 (ab242138, Abcam), SGT1 (11019-2, Proteintech), IL-1β (ab234437, Abcam), Caspase 1 (ab179515, Abcam), cleaved-IL-1β (#63124, CST), cleaved-caspase-1 (#89332, CST), NLRP3 (ab263899, Abcam), ASC (#67824, CST), GAPDH (#5174, CST), and β-actin (sc-69879, Santa Cruze) anti-bodies were used and blots were quantified using Image J Software (NIH, Bethesda, MD, USA). Each group was tested using three samples that were created by mixing the liver samples from mice numbered 1–3, 4–6, and 7–8, respectively. Western blot results were normalized to the GAPDH band or the β-actin band.
Ab242138
Manufactured by Abcam
Sourced in United States
Ab242138 is a lab equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab179515, by Abcam (1 mentions)
Ab59459, by Abcam (1 mentions)
Ab234437, by Abcam (1 mentions)
Ab283654, by Abcam (1 mentions)
P1250, by Applygen (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Ab263899, by Abcam (1 mentions)
Ab179800, by Abcam (1 mentions)
Ab178945, by Abcam (1 mentions)
Ab8227, by Abcam (1 mentions)
Goat anti rabbit igg, by Abcam (1 mentions)
Ab108319, by Abcam (1 mentions)
2 protocols using ab242138
1
Protein Extraction and Western Blotting of Liver Tissues
2
Quantitative Protein Analysis in Brain Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Upon extraction of the total protein from brain tissues, the protein concentration was determined using Bradford protein analysis kits (Beyotime). The equal amount of protein was isolated from each sample using 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride membranes. After blockade with 5% milk in PBS-0.05% Tween, membranes were incubated with the primary antibodies at 4°C overnight, washed three times, and then incubated with the secondary antibody goat anti-rabbit IgG (1 : 2000, Abcam) at room temperature for 90 min. With β-actin as the internal reference, immunoblots were visualized with BeyoECL Plus ECL kits (Beyotime). Primary antibodies were as follows: rabbit monoclonal antibody HSF1 (ab242138, 1 : 1000, Abcam); rabbit monoclonal antibody iNOS (ab178945, 1 : 1000, Abcam), rabbit monoclonal antibody COX-2 (ab179800, 1 : 1000, Abcam), rabbit monoclonal antibody BDNF (ab108319, 1 : 1000, Abcam), and rabbit polyclonal antibody β-actin (ab8227, 1 : 1000, Abcam).
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