Esg gal4

The following fly stocks were used in this study: sina²FRT2A (BDSC, #30724); sina³FRT80B (BDSC, #26270); FRT42D phyl²³⁶⁶ (BDSC, #30723); phyl²²⁴⁵ (Kyoto Stock Center, #108363); FRT2A, FRT80B, FRT42D, Tub-Gal80^ts, UAS-ttk69, Myo1A-Gal4, UAS-Notch-RNAi, and Act<stop<Gal4 (all obtained from BDSC); esg-Gal4 and UAS-GFP, (gift from Shigeo Hayashi, RIKEN Center for Development Biology, Japan); UAS-phyl-RNAi#1 (BDSC, #29433); UAS-phyl-RNAi#2 (VDRC, v35469); UAS-phyl (BDSC, #52015); UAS-sina.myc (BDSC, #30931); UAS-sina-RNAi (VDRC, v100691); UAS-sc (BDSC, #26687); phyl^3.4-nGFP (a gift from Haiwei Pi, Department of Life Science, Chang-Gung University, Taiwan; Pi et al., 2004 (link)); Su(H)-Gal4 and Dl-Gal4 (a gift from Xiankun Zeng and Steven Hou, National Cancer Institute, USA; Zeng et al., 2010 (link)); UAS-N^icd (a gift from Ting Xie, Stowers Institute for Medical Research, USA); UAS-sc-3HA (Fly ORF, F000085) (Bischof et al., 2013 (link)). UAS-ttk69-3xflag was generated in the course of the present study. Briefly, the cDNA of ttk69 was cloned into the gateway attB-PUAST-3xflag vector (Drosophila Genomics Resource Center), and the plasmid was injected into embryos of attP40 flies.

The following stocks were obtained from Bloomington Stock Center bloominton drosophila stock center (BDSC): Canton-S, w¹¹¹⁸, Sirtuin1^2A-7-11 (BDSC, #8838), Sirtuin2^5B-2-35 (BDSC, #8839), Sirtuin4 (BDSC, #8840), DopEcR (BDSC, #10847), and Foxo_RNAi (BDSC, #32427). Dop1R1 (BDSC, #43667), UAS-Kir2.1 (BDSC, #6596), UAS-reaper (BDSC, #5823), nSyb-GAL4 (BDSC, #51635), Ppl-GAL4 (BDSC, #58768), Esg-GAL4 (BDSC, #26816), Adh (BDSC, #3976), Trh^c01440 (BDSC, #10531), Tdc2-GAL4 (BDSC, #9313), GAL4 covering a broader subset of ring neurons (BDSC, #39805), R2,3d,4 m-GAL4 (BDSC, #SS02709), R1-GAL4 (BDSC, #49661), R2-GAL4 (BDSC, #48343), R3d-GAL4 (BDSC, #40074), R4d-GAL4 (BDSC, #48487), R4m-GAL4 (BDSC, #39209), R5-GAL4 (BDSC, #39198), Rel^E38, and Dif mutants were gifts from M. Hedengren. NPF^sk2 was provided by S. Kondo, and ppk28 was a gift from K. Scott. E. Johnson provided the Crz-GAL4 flies. DIlp^2,3,5 and foxo^∆94 were a gift from L. Partridge, and UAS-Tsc1-Tsc2 were from J. H. Lee. Akh, AkhR, and bmm mutant flies were a gift from M. Dus. Aldh^∆24 and CrzR⁰¹ mutant flies were provided by F. James and J. Park, respectively. Tbh^nM18 flies were provided by K. Iliadi, and Poxn^{∆M22−B5∆XB} mutants were provided by J. Alcedo. Gr63a mutant flies were a gift from A. Ray. Serotonin receptor mutant flies were a gift from Y. Rao. Gr63a/orco/Ir8a/Ir25a mutant flies were provided by R. Bento.