Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. For mRNA quantification, cDNA was synthesized using iScript RT Supermix (Bio-Rad), following the manufacturer’s protocol. Quantitative real-time PCR (qRT-PCR) analysis was performed in triplicate using iQ SYBR green Supermix (BioRad) on an iCycler Real-Time Detection System (Biorad). The mRNA level was normalized to GAPDH or 18S as a house keeping gene. For miRNA quantification, total RNA was reverse transcribed using the miScript II RT Kit (Qiagen). Primers specific for human and mouse pre-miR-148a and miR148a (Qiagen) were used and values normalized to SNORD68 (Qiagen) or 18S as a housekeeping gene. For mouse tissues, total liver RNA from WT mice fed a high-fat diet (HFD) were isolated using the Bullet Blender Homogenizer (Next Advance) in TRIzol. 1 μg of total RNA was reverse transcribed and gene/miRNA expression assessed as above. Primer sequences are available upon request.
Primers specific for human and mouse pre mir 148a and mir148a
Manufactured by Qiagen
The Primers specific for human and mouse pre-miR-148a and miR-148a are laboratory equipment used for the detection and analysis of specific microRNA (miRNA) sequences. These primers are designed to target the precursor and mature forms of the miR-148a miRNA, which are found in both human and mouse samples.
Automatically generated - may contain errors
Lab products found in correlation
Iq sybr green supermix, by Bio-Rad (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Iscript rt supermix, by Bio-Rad (2 mentions)
Miscript 2 rt kit, by Qiagen (2 mentions)
Icycler real time detection system, by Bio-Rad (2 mentions)
Snord68, by Qiagen (2 mentions)
Bullet blender homogenizer, by Next Advance (2 mentions)
2 protocols using primers specific for human and mouse pre mir 148a and mir148a
1
Tissue-Specific RNA Expression Analysis
2
Tissue-Specific RNA Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. For mRNA quantification, cDNA was synthesized using iScript RT Supermix (Bio-Rad), following the manufacturer’s protocol. Quantitative real-time PCR (qRT-PCR) analysis was performed in triplicate using iQ SYBR green Supermix (BioRad) on an iCycler Real-Time Detection System (Biorad). The mRNA level was normalized to GAPDH or 18S as a house keeping gene. For miRNA quantification, total RNA was reverse transcribed using the miScript II RT Kit (Qiagen). Primers specific for human and mouse pre-miR-148a and miR148a (Qiagen) were used and values normalized to SNORD68 (Qiagen) or 18S as a housekeeping gene. For mouse tissues, total liver RNA from WT mice fed a high-fat diet (HFD) were isolated using the Bullet Blender Homogenizer (Next Advance) in TRIzol. 1 μg of total RNA was reverse transcribed and gene/miRNA expression assessed as above. Primer sequences are available upon request.
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