Colon tissues and tumors were collected and homogenized into single cell suspension in PBS using an electric homogenizer. Subsequently, cells were lysed in total cell lysis buffer (20 mM HEPES, pH 7.4, 20mM NaCl, 10% glycerol, 1% Triton X-100). Lysates were subjected to standard SDS-polyacrylamide gel electrophoresis (10μg protein/lane) and western blotting procedures using the following primary antibodies: Anti-IRF8 (Cell Signaling, Danvers, MA), Anti-STAT3, anti-pSTAT3, anti-STAT1 and anti-pSTAT1 (BD Biosciences, San Diego, CA), Anti-DNMT1 and Anti-DNMT3b (Abcam, Cambridge, MA). Anti-β-actin (Sigma-Aldrich, St Louis, MO). Anti-Rabbit-HRP (1:5000), Anti-Mouse-HRP (1:5000) secondary antibodies (Cell Signaling, Danvers, MA). Signal was detected using the enhanced chemiluminescence system (ECL; Perkin Elmer, Waltham, MA) and the Xray Film Processor Konica SRX-101A (Konica Minolta, Tokyo, Japan).
Konica srx 101a
Manufactured by Konica Minolta
Sourced in Japan
The Konica SRX-101A is a medical imaging system designed for use in healthcare facilities. It is a digital radiography system that captures and processes X-ray images for diagnostic purposes. The system provides high-quality imaging capabilities to support medical professionals in their clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
Anti irf8, by Cell Signaling Technology (2 mentions)
Anti pstat3, by BD (2 mentions)
Anti pstat1, by BD (2 mentions)
Enhanced chemiluminescence system ecl, by PerkinElmer (2 mentions)
Anti dnmt3b, by Abcam (2 mentions)
Anti dnmt1, by Abcam (2 mentions)
Anti stat1, by BD (2 mentions)
Anti mouse hrp, by Cell Signaling Technology (2 mentions)
Anti rabbit hrp, by Cell Signaling Technology (2 mentions)
Anti β actin, by Merck Group (2 mentions)
Anti stat3, by BD (2 mentions)
4 protocols using konica srx 101a
1
Molecular Profiling of Colon Tissues
2
Radiographic Confirmation of Tibial Cancer
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To confirm cancer development in the tibia, TCI and Sham rats were radiographed on POD 14. Rats were anesthetized with sodium pentobarbital (50 mg/kg, i.p.), and placed prone on an X-ray film (Henry Schein blue sensitive film, Henry Schein, Melville, NY, United States), and then exposed to an X-ray source (Emerald 125) for 1/20 s at 40 KVP. The X-ray film was developed in a film developer (Konica SRX-101A, Konica Minolta, Tokyo, Japan).
3
Radiographic Assessment of TCI-Induced Bone Destruction
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To confirm TCI-induced bone destruction, the tibia of rats were radiographed on POD 21. They were placed supine on an X-ray film (Henry Schein blue sensitive film, Henry Schein, NY, USA) after analgesia and were exposed to an X-ray source (Emerald 125) for 1/20 s at 40 KVP. The X-ray film was developed by a film developer (Konica SRX-101A, Konica Minolta, Tokyo, Japan).
4
Molecular Profiling of Colon Tissues
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