Sf9 insect cell line

Manufactured by American Type Culture Collection

The SF9 insect cell line is a commonly used host cell line for the expression of recombinant proteins. It is derived from the fall armyworm (Spodoptera frugiperda) ovarian cells and is known for its ability to produce high levels of recombinant proteins.

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Lab products found in correlation

Quikchange 2 xl, by Agilent Technologies (1 mentions)

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Sf9 cells (32 citations)

2 protocols using sf9 insect cell line

Generating ERAP1 Variant Expression Constructs

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Sequence variants of ERAP1 were generated (inserted in pFastBac plasmid) using Agilent QuikChange II XL site-directed mutagenesis. Primers used for site-directed mutagenesis of ERAP1 are listed in Supplementary Table 4. All ERAP1 variants were validated by sequencing the entire open reading from in the expression plasmid to confirm identity and lack of unexpected mutations. Positive clones were used to make bacmids and baculoviral stocks were prepared using SF9 insect cell line (CRL-1711, ATCC) following Bac-to-Bac baculoviral system protocol.

Maben Z., Arya R., Georgiadis D., Stratikos E, & Stern L.J. (2021). Conformational dynamics linked to domain closure and substrate binding explain the ERAP1 allosteric regulation mechanism. Nature Communications, 12, 5302.

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Recombinant Hemagglutinin Production

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Recombinant HAs were produced using recombinant baculoviruses expressing soluble HAs with trimerization domains and hexahistidine tags. An Sf9 insect cell line (ATCC CRL-1711) was used to propagate the baculovirus, which was then used to infect BTI-TN-5B1-4 cells, for efficient secretion of recombinant HA. Recombinant HA was purified from cell supernatant using Ni-nitrilotriacetic acid resin columns. The procedure is described in detail in published protocols (35 (link), 36 (link)).

Meade P., Kuan G., Strohmeier S., Maier H.E., Amanat F., Balmaseda A., Ito K., Kirkpatrick E., Javier A., Gresh L., Nachbagauer R., Gordon A, & Krammer F. (2020). Influenza Virus Infection Induces a Narrow Antibody Response in Children but a Broad Recall Response in Adults. mBio, 11(1), e03243-19.

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