Fluticasone propionate (FP), lipopolysaccharide from Escherichia coli (LPS), and propidium iodide were purchased from Sigma-Aldrich (St. Louis, MO, USA). IC87114, an inhibitor of PI3Kδ, was purchased from Chemdea (Ridgewood, NJ, USA). Fluorescein isothiocyanate (FITC)-conjugated anti-neutrophils (7/4) antibody was purchased from Acris Antibodies GmbH (Herford, Germany). Anti-Nrf2 (ab31163) and anti-HDAC2 (ab12169) antibodies were purchased from Abcam plc (Cambridge, UK). Anti-Akt antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-phospho-Akt (Ser473), clone SK703, was purchased from Merck Millipore Co. (Darmstadt, Germany). Anti-β-actin (AC-15) antibody was purchased from Sigma-Aldrich Co, LLC (St. Louis, MO, USA). HRP-conjugated anti-rabbit IgG and anti-mouse IgG and ECL Plus were purchased from GE Healthcare UK Ltd. (Buckingham, UK).
Anti nrf2 ab31163
Manufactured by Abcam
Sourced in United Kingdom, United States
Anti-Nrf2 (ab31163) is a primary antibody that recognizes the Nrf2 protein. Nrf2 is a transcription factor that plays a key role in the regulation of genes involved in the antioxidant response and cellular detoxification processes. This antibody can be used in various applications, such as Western blotting, to detect and study the Nrf2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin antibody ac 15, by Merck Group (1 mentions)
Anti akt antibody, by Cell Signaling Technology (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Ecl plus, by GE Healthcare (1 mentions)
Zen 2010 program version 6, by Zeiss (1 mentions)
Fluorescent mounting solution, by Agilent Technologies (1 mentions)
Dako antibody diluent, by Agilent Technologies (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Ab6640, by Abcam (1 mentions)
Anti phosphorylated smad1 5 8, by Cell Signaling Technology (1 mentions)
Anti phosphorylated p38, by Cell Signaling Technology (1 mentions)
Anti phosphorylated erk, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Anti phosphorylated p53, by Cell Signaling Technology (1 mentions)
Anti phosphorylated stat3, by Cell Signaling Technology (1 mentions)
Anti phosphorylated ampk, by Cell Signaling Technology (1 mentions)
Rat anti mouse cd44 clone im7, by BD (1 mentions)
Anti phosphorylated mtor, by Cell Signaling Technology (1 mentions)
Anti α sma ab5694, by Abcam (1 mentions)
Anti ampk, by Cell Signaling Technology (1 mentions)
4 protocols using anti nrf2 ab31163
1
Studying LPS-Induced Inflammation and Nrf2 Regulation
2
Nrf2 Immunohistochemistry in Brain Tissue
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A total of 5 μm thick sections of routine paraffin embedded brain tissues were used. Anti-Nrf2 (#ab31163; Abcam, MA, USA) proteins were analyzed following protocols from earlier studies (Ebokaiwe et al. 2019 , 2020a , b ).
3
Nrf2 Immunofluorescence in Astrocytes
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Astrocytes were washed with PBS and fixed with 4% paraformaldehyde for 30 min. Cells were then incubated in blocking buffer (2% BSA, .2% nonfat milk, .4% Triton X100 in PBS) for 1 h and then incubated for 3 h with the primary antibody anti-Nrf2 (AB 31163) (1:50) (Abcam, Cambridge, MA). Cells were washed 3 times with PBS-Tween (.2%) and subsequently incubated for 1 h with the ALEXA FLUOR 594 anti-rabbit secondary antibody (1:200). Four washes were performed with PBS-Tween and the nuclei were stained with DAPI (1ug/mL) for 5 min. Slides were mounted with fluorescent mounting solution (DakoCytomation, Glostrup, Denmark). Single plane images were obtained in a confocal microscope LSM-META-Zeiss Axioplan 2 (40X); the 405 nm Laser Diode was used for the DAPI chromophore; and the Ar/ML 458/488/514 nm for the ALEXA 594. The analysis of the images was performed using the ZEN 2010 program version 6.0 (Carl Zeiss).
4
Comprehensive Kidney Tissue Immunostaining Protocol
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For immunofluorescence staining of kidney tissues, the antibodies used were guinea pig anti-Nephrin (clone GP-N2, Progen, Heidelberg, Germany), mouse anti-synaptopodin (clone G1D4, Progen), and rat anti-mouse CD44 (clone IM7, BD Biosciences). For immunohistochemistry, the antibodies used were rat anti-F4/80 (ab6640, Abcam, Cambridge, UK), rabbit anti-WT1 (clone C-19, Santa Cruz Biotechnology, CA, USA), rabbit anti-α-SMA (ab5694, Abcam), and mouse anti-PCNA (clone PC10, DAKO). All antibodies were diluted at 1:100 in DAKO antibody diluent (Agilent, CA, USA). The following polyclonal rabbit antibodies used for primary reaction of immunoblots were obtained from Cell Signaling Technology (MA, USA): anti-phosphorylated Smad1/5/8 (#95115), anti-phosphorylated AMPK (#2531), anti-AMPK (#2532), anti-phosphorylated p38 (#4511), anti-p38 (#9212), anti-phosphorylated ERK (#4370), anti-ERK (#4695), anti-phosphorylated STAT3 (#9145), anti-phosphorylated p53 (#9284), and anti-phosphorylated mTOR (#2971). Rabbit antibodies anti-α-SMA (ab5694) and anti-NRF2 (ab31163) were from Abcam. Rabbit antibodies anti-STAT3 (clone C-20), anti-p53 (clone FL393), and anti-vinculin (clone H-300) were obtained from Santa Cruz Biotechnology.
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