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Sodium dihydrogen phosphate

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Sourced in Spain

Sodium dihydrogen phosphate is a soluble inorganic salt that is commonly used as a buffering agent in laboratory applications. It is a white, crystalline powder that is highly soluble in water. The primary function of sodium dihydrogen phosphate is to maintain a specific pH level in solutions, ensuring a stable and controlled environment for various experiments and analytical procedures.

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8 protocols using sodium dihydrogen phosphate

1

Tetracycline Derivatives and Redox-Active Compounds

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Tetracycline hydrochloride (TET), chlorTetracycline hydrochloride (CTC) doxycycline hydrochloride (DOX), oxyTetracycline hydrochloride (OTC), hexaammineruthenium(II) chloride, hexaammineruthenium(III) chloride, graphene oxide (GO, ref:763705) and trichloroacetic acid were purchased from Sigma-Aldrich (https://www.sigmaaldrich.com). Disodium hydrogen phosphate and sodium dihydrogen phosphate were purchased from Panreac (http://www.panreac.com). Ascorbic acid was purchased from Fluka Chemie (via Sigma-Aldrich, Darmstadt, Germany). All solutions were prepared in Milli-Q water (https://www.merckmillipore.com). Skim milk was acquired in a Spanish market. River water samples were collected from Henares River (Spain).
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2

Analysis of Pharmaceutical Compounds

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Chemicals and reagents used in this research were of analytical grade. Chitosan of 310,000–375,000 Da molecular weight, MeP, EtP, PrP, iPrP, BuP, iBuP, BzP, KTP, NAX, SAL, MRB, ENR, and FLM were acquired from Fluka-Sigma-Aldrich (Madrid, Spain). HPLC-grade methanol, acetic acid, and hydrochloric acid were obtained from Merck (Darmstadt, Germany). Sodium hydroxide and sodium dihydrogen phosphate were obtained from Panreac (Barcelona, Spain). Working standard solutions of parabens, NSAIDs, and FQs were daily prepared by adequate dilutions in ultrapure water from methanolic stock solutions (400 mg L−1). A Milli-Q water purification system (Millipore, Bedford, MA, USA) was employed to provide ultra-high-purity water.
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3

Phaeophyceae Seaweed Bioactivity Evaluation

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Grounded F. vesiculosus samples from July 2017 were purchased from Algaplus Lda (production site located at Ria de Aveiro coastal lagoon, Northern Portugal, 40°36′43″ N, 8°40′43″ W) an enterprise dedicated to the production of edible seaweeds in an integrated multi-trophic aquaculture (IMTA) system. HPLC grade acetone, ethanol, methanol, n-hexane, ethyl acetate, acetonitrile, dimethylsulfoxide, hydrochloric acid, glacial acetic acid, sodium chloride, sodium hydroxide, potassium hydroxide, sodium and potassium tartarate, tris-HCl, and starch were acquired from Fisher (Pittsburgh, PA, USA). The enzymes α-glucosidase from Saccharomyces cerevisiae (EC No. – 3.2.1.20), α-amylase from porcine pancreas (EC No. – 3.2.1.1) and lipase from porcine pancreas (EC No. – 3.1.1.3) together with 2,4-dimethoxybenzaldehyde (DMBA), 4-nitrophenyl α-D-glucopyranoside (PNPG), 4-nitrophenyl butyrate (PNPB) and formic acid were purchased from Sigma (St. Louis, MO, USA). Sodium di-hydrogen phosphate and potassium di-hydrogen phosphate were acquired from Panreac (Barcelona, Spain). Dinitrosalycilic acid and Acarbose were purchased from Acros Organics (Hampton, NH, USA), calcium chloride from ChemLab (Eernegem, Belgium) and orlistat from AlfaAesar (Ward Hill, MA, USA). All reagents were of analytical grade or of the highest available purity.
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4

Synthesis and Characterization of CNC/SWCNT Hybrids

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K4Fe(CN)6·3H2O, K3Fe(CN)6, Ru(NH3)6Cl3, N,N-dimethylformamide (DMF), alpha-1-acid glycoprotein (AGP) (≥ 99%), transferrin (≥ 98%), human serum (certified reference material, ERM-DA470K/IFCC), potassium osmate (VI) dihydrate, N,N,N′,N′-tetramethylenediamine (TEMED), potassium nitrate (≥ 98%), uric acid, dopamine, l-tyrosine, and cellulose microcrystalline powder (20 µm) were purchased from Sigma–Aldrich (Darmstadt, Germany). Hydrochloric acid (37%), sulfuric acid (95–98%), sodium chloride, disodium hydrogen phosphate, and sodium dihydrogen phosphate were purchased from PanReac (Barcelona, Spain).
SWCNTs were acquired from Carbon Solutions Inc. (type P2-SWNT, Riverside, CA, USA). These correspond to a purified material following a non-specified non-oxidative process.
For the synthesis of CNC and characterization of CNC/SW hybrids, ultrapure water was obtained from a Siemens Ultraclear device, with a conductivity of 0.055 μS cm−1. For the analytical studies, all aqueous solutions were prepared in Milli-Q water (Merck Millipore, Darmstadt, Germany).
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5

Inhibition of Multicopper Oxidases

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All chemicals used were analytical grade and were used as purchased. 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sodium fluoride, 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO), quinhydrone, 2,6-dimethoxyphenol (DMP) and sodium azide were Scheme 1 Scheme of proposed binding sites of azide and halide inhibitors to MCOs active site 1 3 purchased from Sigma. Sodium chloride, potassium chloride, di-sodium hydrogen phosphate and sodium di-hydrogen phosphate were purchased from Panreac. All aqueous solutions were prepared in deionized water (Milli-Q grade, Millipore).
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6

Trace Metal Analysis Methodology

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Organic solvents (i.e., 1-undecanol, chloroform, 1-butyl-3-methylimidazolium hexafluorophosphate, acetone, methanol, absolute ethanol and 1-propanol) and chelating agents (i.e. diethyldithiocarbamate (DDTC), 2-theonyltrifluoroacetone (TTA), ammonium pyrrolidine dithiocarbamate (APDC) and a multi-element 200 mg L -1 organometallic solution were purchased from Sigma-Aldrich (Steinheim, Germany). Sodium chloride, 69% w w -1 nitric acid, 36% w w -1 hydrochloric acid, 85% w w -1 phosphoric acid, sodium dihydrogen phosphate, acetic acid and sodium acetate were obtained from Panreac (Barcelona, Spain). An ICP-IV multi-element 1000 mg L -1 solution was provided by Merck (Darmstadt, Germany).
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7

Quantification of Phytochemicals in Foods

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All the chemicals and solvents used were of analytical grade. Oxalic acid, fumaric acid, tartaric acid, malic acid, citric acid, lactic acid, D-(+)-fructose, D-(+)-glucose, sucrose, gallic acid 1hydrate, Folin-Ciocalteu reagent, sodium dihydrogen phosphate, sodium carbonate anhydrous, HPLC-grade acetonitrile and 37% (v/v) chloridric acid were purchased from Panreac Applichem (IWT group, Barcelona, Spain). Methanol and anhydrous di-sodium hydrogen phosphate were supplied by Scharlau Chemie (Scharlab S.L. Barcelona, Spain). Thermostable amyloglucosidase (GOPOD) reagent buffer, GOPOD reagent enzymes, D-(+)-glucose standard solution, amyloglucosidase and pancreatic-α-amylase were purchased from Megazyme International (Ireland). Fluorescein, 2, 2-azobis (2-amidinopropane) dihydrochloride (AAPH) and 6-hydroxy-2, 5, 7, 8-tetramethylchromane-2-carboxylic acid (Trolox) were obtained from Acros Organics (Thermo Fisher Scientific Inc. Waltham, MA, USA).
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8

Quantification of LND in Human Plasma

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All reagents were of analytical grade. LND, 3-(4-amino-1,3-dihydro-1-oxo-2H-isoindol-2-yl)-2,6-piperidinedione, was purchased from Cayman Chemical (Ann Arbor, MI) and stored at -20 °C. Sodium dihydrogen phosphate was purchased from Panreac (Barcelona, Spain). Purified water was purchased from Grifols (Barcelona, Spain). Ortophosporic acid was obtained from Fluka (St. Louis, MO). Acetonitrile and methanol were obtained from Scharlab (Barcelona, Spain). Human plasma samples were collected from healthy volunteers at Doctor Peset University Hospital (Valencia, Spain) and kept frozen at -20 °C until analysis.
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