Anti cd105 apc

The myogenic nature of purified cells was evaluated by flow cytometry analysis performed on CytoFLEX (Beckman Coulter) as previously described (Awaya et al., 2012 (link); Lapan and Gussoni, 2012 (link)); the following panel of antibodies was used to determine immunophenotype: anti-CD56 PC7 (Beckman Coulter, USA, A21692), anti-CD146 PE (Beckman Coulter, USA, A07483), anti-CD166 PE (Beckman Coulter, USA, A22361), anti-CD73 PE (BD Pharmingen, USA, 550257), anti-CD105 APC (R&D Systems, USA, FAB1097A-100), and anti-CD45 PC5 (Beckman Coulter, USA, A07785). Data were analyzed using the CytExpert 2.0 (Beckman Coulter). The phenotypic characteristics of the obtained cells are illustrated in the Supplementary Material, Figure S2A.

The immunophenotype of stem cells was evaluated by flow cytometry analysis performed on CytoFLEX (Beckman Coulter). Сells were resuspended in 100 μL of PBS containing 1% of bovine serum albumin (Sigma-Aldrich, Saint Louis, MO, USA) and incubated for 20 min at 20°C in the dark with the following monoclonal antibodies (Ab): anti-CD56 PC7 (Beckman Coulter, USA, A21692), anti-CD146 PE (Beckman Coulter, USA, A07483), anti-CD166 PE (Beckman Coulter, USA, A22361), anti-CD73 PE (BD Pharmingen, USA, 550257), anti-CD105 APC (R&D Systems, USA, FAB1097A-100), anti-CD45 PC5 (Beckman Coulter, USA, A07785), anti-PDGFRβ APC (BD Pharmingen, USA, FAB1263A), and anti-CD140a PE (BioLegend, USA, 323506). Data were analyzed using the CytExpert 2.0 (Beckman Coulter).