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Horseradish peroxidase streptavidin sa 5004

Manufactured by Vector Laboratories
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Horseradish peroxidase streptavidin (SA-5004) is a conjugate of streptavidin and horseradish peroxidase. Streptavidin is a tetrameric protein that binds biotin with high affinity. Horseradish peroxidase is an enzyme that catalyzes oxidation-reduction reactions.

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Lab products found in correlation

M0630, by Merck Group (2 mentions) Ez run prestained protein ladder bp3603, by Thermo Fisher Scientific (2 mentions) Natural unstained protein ladder 161 0317, by Bio-Rad (2 mentions) B2787, by Bio-Rad (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Thermomixer, by Eppendorf (2 mentions) Biotinyl asp glu val asp aldehyde, by Bachem (2 mentions)

3 protocols using horseradish peroxidase streptavidin sa 5004

1

Preparation and Analysis of Homocysteinylated Myoglobin

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Horseradish peroxidase streptavidin (SA-5004) was from Vector Laboratories (Burlingame, CA, USA). SuperSignal West Pico chemiluminescent substrate was from Thermo Scientific (Rockford, IL, USA). Myoglobin from equine skeletal muscle (M0630) was from Sigma-Aldrich. N-Homocysteinylated myoglobin was prepared as we previously reported [64 (link)]. Biotinyl-Asp-Glu-Val-Asp-aldehyde (CAS registry number: 178603-73-1) was from Bachem Americas (N-1470, Torrance, CA, USA; see Figure S1 for its structure in Support Information). Rhodamine-aldehyde (10 mM) in 50% 200-proof ethanol was synthesized as previously described [64 (link)]. EZ-Run prestained protein ladder (BP3603) was from Fisher Scientific. Natural unstained protein ladder (161-0317) was from Bio-Rad. Biotinylated protein markers were from Sigma-Aldrich (B2787) and Bio-Rad (161-0319), respectively. All reagents were ACS grade and used as received without further purification. All incubations were carried out in an Eppendorf Thermomixer® (Eppendorf North America, Hauppauge, NY, USA) at 25 °C unless specified otherwise.
Zang T., Pottenplackel L.P., Handy D.E., Loscalzo J., Dai S., Deth R.C., Zhou Z.S, & Ma J. (2016). Comparison of Protein N-Homocysteinylation in Rat Plasma under Elevated Homocysteine Using a Specific Chemical Labeling Method. Molecules, 21(9), 1195.
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2

Homocysteinylated Myoglobin Labeling Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Horseradish peroxidase streptavidin (SA-5004) was from Vector Laboratories (Burlingame, CA, USA). SuperSignal West Pico chemiluminescent substrate was from Thermo Scientific (Rockford, IL, USA). Myoglobin from equine skeletal muscle (M0630) was from Sigma-Aldrich. N-Homocysteinylated myoglobin was prepared as we previously reported [64 (link)]. Biotinyl-Asp-Glu-Val-Asp-aldehyde (CAS registry number: 178603-73-1) was from Bachem Americas (N-1470, Torrance, CA, USA; see Figure S1 for its structure in Support Information). Rhodamine-aldehyde (10 mM) in 50% 200-proof ethanol was synthesized as previously described [64 (link)]. EZ-Run prestained protein ladder (BP3603) was from Fisher Scientific. Natural unstained protein ladder (161-0317) was from Bio-Rad. Biotinylated protein markers were from Sigma-Aldrich (B2787) and Bio-Rad (161-0319), respectively. All reagents were ACS grade and used as received without further purification. All incubations were carried out in an Eppendorf Thermomixer® (Eppendorf North America, Hauppauge, NY, USA) at 25 °C unless specified otherwise.
Zang T., Pottenplackel L.P., Handy D.E., Loscalzo J., Dai S., Deth R.C., Zhou Z.S, & Ma J. (2016). Comparison of Protein N-Homocysteinylation in Rat Plasma under Elevated Homocysteine Using a Specific Chemical Labeling Method. Molecules, 21(9), 1195.
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3

Immunohistochemical Analysis of PCNA

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For the immunohistochemical studies, the sections were incubated with primary PCNA antibody (1:500, AV03018, Sigma-Aldrich, St. Louis, MO, USA) overnight at 4°C. Then, the sections were incubated with goat anti-rabbit IgG and biotin-conjugated antibody (1:300, CW0107, CWBioTech, Beijing, China) for 2 h 30 min at room temperature. Horseradish peroxidase streptavidin (SA-5004, Vector, Germany) and a diaminobenzidine (DAB) kit (ZLI-9017, ZsBio, Beijing, China) were used for staining and imaging. The mean density (IOD/area) of positive cells was measured from 3 random fields of 5 sections in each sample using Image-Pro Plus 6.0.
Xu W., Lu J., Chen Y., Wang Z., Cao J, & Dong Y. (2021). Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress. Endocrine journal, 68(4).
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