Recombinant human fb growth factor

We used HaCaT cells, HS27 fibroblasts (FBS), and HUVECs as substitutes for the epithelial cells, FBS and blood vessel endothelial cells present in skin tissue54 (link). The human epidermal keratinocytes (HaCaT, ATCC^®, USA) and human skin Fb HS27 cells (CRL-1634, ATCC^®, USA) were cultured in high-glucose DMEM (WELGENE, South Korea) supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY) and 1% penicillin/streptomycin solution (Gibco, Grand Island, NY). The HUVECs (PCS-100-010^™, ATCC^®, USA) were cultured in EGM-2 supplemented with ascorbic acid, vascular endothelial growth factor, 20% FBS, recombinant human Fb growth factor, hydrocortisone, insulin-like growth factor-1, a recombinant analog of human insulin-like growth factor-1, gentamicin–amphotericin, and heparin (Lonza, Walkersville, MD, USA). All experiments used cells from passages 3–10.

Human epidermal keratinocytes HaCaT (Addexbio, San Diego, CA, USA), a spontaneously transformed immortal human keratinocyte cell line, and human skin fibroblasts Hs27 (CRL1735, ATCC^®, Manassas, VA, USA) were cultured in high-glucose DMEM (WELGENE, Daegu, Korea) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin solution (Gibco). The HUVECs (PCS-100-01, ATCC^®) were cultured in EGM-2 supplemented with ascorbic acid, vascular endothelial growth factor, 20% FBS, recombinant human Fb growth factor, hydrocortisone, insulin-like growth factor-1, a recombinant analog of human insulin-like growth factor-1, gentamicin–amphotericin, and heparin (Lonza, Basel, Switzerland). HaCaT, Hs27, and HUVECs were used as substitutes for epithelial cells, fibroblasts, and blood vessel endothelial cells present in the skin tissue. All experiments used cells from passages 3–10. All cells were incubated in 5% CO₂ at 37 °C.