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Mirna mimic or inhibitor

Manufactured by RiboBio
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The MiRNA mimic or inhibitor is a lab equipment product designed for the modulation of microRNA (miRNA) expression. It can be used to either increase or decrease the levels of specific miRNAs in a sample, enabling researchers to study their functional roles. The product provides a reliable and efficient tool for manipulating miRNA levels in various experimental settings.

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Lab products found in correlation

Turbofect transfection reagent, by Thermo Fisher Scientific (1 mentions) Ribofect cp reagent, by RiboBio (1 mentions) Ribofect cp buffer, by RiboBio (1 mentions)

Spelling variants of this product

MiRNA mimics (108 citations) MiRNA inhibitors (47 citations) MiRNA mimic/inhibitor (5 citations) MiRNA mimics or inhibitors (4 citations)

2 protocols using mirna mimic or inhibitor

1

Plasmid and miRNA Transfection Protocol

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The transfection of plasmid DNA, miRNA mimic or inhibitor was performed by TurboFect Transfection Reagent (Thermo Fisher Scientific) with the manufacture's protocol. Briefly, 100 nM miRNA mimic or inhibitor (purchased from Guangzhou RiboBio Co., Ltd.) or 1 μg of pCMV14-3X flag or pCMV-BMI1-flag was complexed with transfection reagent as a ratio of 1 μg nuclei acid: 2 μl reagent at room temperature for 15 minutes and then added into wells of 6-well-plates with cell attachment at 60% confluency. Cells were then harvested at 48h post transfection for further experiments.
Chen S.M., Wang B.Y., Lee C.H., Lee H.T., Li J.J., Hong G.C., Hung Y.C., Chien P.J., Chang C.Y., Hsu L.S, & Chang W.W. (2017). Hinokitiol up-regulates miR-494-3p to suppress BMI1 expression and inhibits self-renewal of breast cancer stem/progenitor cells. Oncotarget, 8(44), 76057-76068.
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2

miRNA Transfection Optimization Protocol

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Cells were transfected with miRNA mimic or inhibitor (Ribobio) according to the manufacturer’s instructions. Briefly, cells were plated in 6-well plates and grown to 70 % confluence. For each well, 50 nM miR-133b mimic or 100 nM inhibitor in riboFECT™ CP Buffer (Ribobio) was mixed gently with riboFECT™ CP Reagent and added to the cells for 12 h.
Zhuang Q., Zhou T., He C., Zhang S., Qiu Y., Luo B., Zhao R., Liu H., Lin Y, & Lin Z. (2016). Protein phosphatase 2A-B55δ enhances chemotherapy sensitivity of human hepatocellular carcinoma under the regulation of microRNA-133b. Journal of Experimental & Clinical Cancer Research : CR, 35, 67.
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