Log In Sign up
The largest database of trusted experimental protocols

Elisa max deluxe set human il 10

Manufactured by BioLegend
Visit Supplier
Sourced in United States

The ELISA Max Deluxe Set Human IL-10 is a laboratory equipment product designed for the quantitative measurement of human interleukin-10 (IL-10) levels in various sample types.

Automatically generated - may contain errors

Lab products found in correlation

Ifn γ, by BioLegend (2 mentions) Elisa max deluxe set human il 2, by BioLegend (1 mentions) Elisa max deluxe set human ifn γ, by BioLegend (1 mentions) Elisa max deluxe set human il 6, by BioLegend (1 mentions) Tnf α, by BioLegend (1 mentions) Interleukin 4 (il 4), by BioLegend (1 mentions) Human ifn gamma uncoated elisa, by Thermo Fisher Scientific (1 mentions) Elisa max deluxe set human cxcl10 ip 10, by BioLegend (1 mentions)

4 protocols using elisa max deluxe set human il 10

1

Cytokine Secretion Profiling of CAR-T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The E:T = 3:1 in vitro killing conditioned medium (as described in Section 2.4) was collected and divided into the T cell group, anti-CD19 CAR-T cell group, single KD anti-CD19 CAR-T cell group, double KD anti-CD19 CAR-T cell group, and tumor cell negative control group (1:1 mixture of K562 and K562-CD19 only). The secretion of IL-6, IFN-γ, IL-17, IL-2 and IL-10 was detected using ELISA MAX™ Deluxe Set Human IL-6 (Biolegend, Cat: 430504), ELISA MAX™ Deluxe Set Human IFN-γ (Biolegend, Cat: 430104), ELISA MAX™ Deluxe Set Human IL-17A kit (Biolegend, Cat: 433914), ELISA MAX™ Deluxe Set Human IL-2 (Biolegend, Cat: 431804), and ELISA MAX™ Deluxe Set Human IL-10 (Biolegend, Cat: 430604), respectively, following the manufacturer’s instructions.
Zhang H., Lv X., Kong Q, & Tan Y. (2022). IL-6/IFN-γ double knockdown CAR-T cells reduce the release of multiple cytokines from PBMCs in vitro. Human Vaccines & Immunotherapeutics, 18(1), 1-14.
+ Open protocol
+ Expand
2

Quantifying Cytokine Levels in Splenocyte Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercially available kits ELISA Max Deluxe Set Human IL-10 (Cat No. 430605), IFN-γ (Cat No. 430105), TNF-α (Cat No. 430204) and IL-4 (Cat No. 430304) (Biolegend, U.S.A) were used to estimate concentration of cytokines in splenic culture supernatants, using the manufacturer’s instructions. The manufacturer-reported detection sensitivities for ELISA MAX Deluxe Set kits are 2 pg/mL for IL-10, TNF-α and IL-4 whereas 4 pg/mL for IFN-γ cytokine.
Singh N, & Sundar S. (2018). Combined neutralization of interferon gamma and tumor necrosis factor alpha induces IL-4 production but has no direct additive impact on parasite burden in splenic cultures of human visceral leishmaniasis. PLoS ONE, 13(6), e0199817.
+ Open protocol
+ Expand
3

Cytokine and Chemokine Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentration of TNFα, IL1β, IL10, CXCL10, IL17, and IFNγ in the cell supernatants was measured using an enzyme-linked immunosorbent assay (ELISA) according to the manufacturers’ protocols (Human TNFα Uncoated ELISA, eBioscience, San Diego, CA; Human IL1β Uncoated ELISA, eBioscience; ELISA MAX Deluxe Set Human IL10, BioLegend, San Diego, CA; ELISA MAX Deluxe Set Human CXCL10 (IP-10), BioLegend; Human IL17A alpha Uncoated ELISA, Invitrogen; Human IFN gamma Uncoated ELISA, Invitrogen, Waltham, MA). LPS concentration in the FS samples was assessed by the LPS ELISA Kit (Aviva Systems Biology, San Diego, CA). The concentration of calprotectin in fecal samples was determined by a sandwich ELISA (Calprotectin ELISA; Bühlmann Laboratories AG, Basel, Switzerland).
Maasfeh L., Härtlova A., Isaksson S., Sundin J., Mavroudis G., Savolainen O., Strid H., Öhman L, & Magnusson M.K. (2021). Impaired Luminal Control of Intestinal Macrophage Maturation in Patients With Ulcerative Colitis During Remission. Cellular and Molecular Gastroenterology and Hepatology, 12(4), 1415-1432.
+ Open protocol
+ Expand
4

Quantifying IL-10 and IFN-γ by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-10 and IFN-γ concentrations were measured in plasma using ELISA Max Deluxe Set Human IL-10 (Cat No. 430605) and IFN-γ (Cat No. 430105)Biolegend’s commercial kit(US), as per manufacturer’s protocol.
Singh N, & Sundar S. (2017). Inflammatory chemokines and their receptors in Human Visceral Leishmaniasis:Gene expression profile in peripheral blood, splenic cellular sources and their impact on trafficking of inflammatory cells. Molecular immunology, 85, 111-119.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!