RNA was extracted using an RNeasy kit. cDNAs were synthesized using the first-strand cDNA synthesis kit. Quantitative real-time PCR was performed using Taqman probes for human NIS and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with assay IDs Hs00166567_m1 and Hs02758991_g1, respectively, on the 7900HT PCR cycler (Applied Biosystems, USA). Triplicate samples were run for each sample. The comparative ΔΔCt method was used to calculate relative gene expression.
7900ht pcr cycler
Manufactured by Thermo Fisher Scientific
Sourced in United States
The 7900HT PCR cycler is a high-throughput real-time PCR system designed for a wide range of applications. It features a 384-well block format and is capable of performing fast and standard PCR protocols. The system is equipped with 96-well and 384-well sample block options and supports a temperature range of 4°C to 100°C.
Automatically generated - may contain errors
2 protocols using 7900ht pcr cycler
1
Quantitative Analysis of NIS Expression
2
Quantifying NIS Expression in HDACi-Treated Cells
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After the cells were treated with HDACi for 48 hours, RNA was extracted using RNeasy kit (QIAGEN, USA). cDNAs were synthesized using the first strand cDNA synthesis kit (Invitrogen, USA). Quantitative real-time PCR was performed using Taqman probe mix on the 7900HT PCR cycler (Applied Biosystems, USA). The Taqman probes for human NIS and GAPDH with assay ids Hs00166567_m1 and Hs02758991_g1 respectively were used (Applied Biosystems, USA). Triplicate samples were run for each sample. The comparative ΔΔCt method was used to calculate relative gene expression.
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