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Proteinase k

Manufactured by Vector Laboratories
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Proteinase K is a broad-spectrum serine protease commonly used in molecular biology applications. It effectively digests and inactivates a wide range of proteins, including enzymes, structural proteins, and DNA-binding proteins. This makes it a valuable tool for sample preparation, DNA extraction, and other applications where the removal of proteins is required.

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Lab products found in correlation

Abc kit, by Vector Laboratories (5 mentions) Antigen unmasking solution, by Vector Laboratories (5 mentions) Nis element software, by Nikon (5 mentions) Microscope image system, by Nikon (5 mentions)

5 protocols using proteinase k

1

Immunohistochemical Staining Protocol

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Immunohistochemical staining was performed on paraffin sections. Antigen retrieval was performed with antigen unmasking solution (Vector Laboratories) or proteinase K. Endogenous peroxidase activity was quenched with 3% H2O2 for 10 min. After blocking with 5% normal serum, slides were incubated with primary antibodies in a humidified chamber overnight. After washing, slides were incubated with appropriate secondary antibodies and ABC solution sequentially according to the ABC kit (Vector Laboratories). Slides were then visualized by incubation in diaminobenzidine solution for an appropriate period of time. Nuclear staining was performed with hematoxylin. The slides were dehydrated, cleared, and mounted. The images from these slides were obtained and analyzed by NIS Element software (Nikon Instruments) with Nikon microscope image system (Nikon Instruments).
Liang H., Zhang Z., He L, & Wang Y. (2016). CXCL16 regulates cisplatin-induced acute kidney injury. Oncotarget, 7(22), 31652-31662.
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2

Immunohistochemical Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical staining was performed on paraffin sections. Antigen retrieval was performed with antigen unmasking solution (Vector Laboratories) or proteinase K. Endogenous peroxidase activity was quenched with 3% H2O2 for 10 min. After blocking with 5% normal serum, slides were incubated with primary antibodies in a humidified chamber overnight. After washing, slides were incubated with appropriate secondary antibodies and ABC solution sequentially according to the ABC kit (Vector Laboratories). Slides were then visualized by incubation in diaminobenzidine solution for an appropriate period of time. Nuclear staining was performed with hematoxylin. The slides were dehydrated, cleared, and mounted. The images from these slides were obtained and analyzed by NIS Element software (Nikon Instruments) with Nikon microscope image system (Nikon Instruments).
Liang H., Zhang Z., Yan J., Wang Y., Hu Z., Mitch W.E, & Wang Y. (2017). The IL-4 receptor α has a critical role in bone marrow-derived fibroblast activation and renal fibrosis. Kidney international, 92(6), 1433-1443.
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3

Immunohistochemical Staining Protocol

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Immunohistochemical staining was performed on paraffin sections. Antigen retrieval was performed with antigen unmasking solution (Vector Laboratories) or proteinase K. Endogenous peroxidase activity was quenched with 3% H2O2 for 10 min. After blocking with 5% normal serum, slides were incubated with primary antibodies in a humidified chamber overnight. After washing, slides were incubated with appropriate secondary antibodies and ABC solution sequentially according to the ABC kit (Vector Laboratories). Slides were then visualized by incubation in diaminobenzidine solution for an appropriate duration of time. Nuclear staining was performed with hematoxylin. The slides were dehydrated, cleared, and mounted. The images from these slides were obtained and analyzed by NIS Element software (Nikon Instruments) with the Nikon microscope image system (Nikon Instruments).
Fan Y., Chen H., Peng H., Huang F., Zhong J, & Zhou J. (2017). Molecular Mechanisms of Curcumin Renoprotection in Experimental Acute Renal Injury. Frontiers in Pharmacology, 8, 912.
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4

Immunohistochemical Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical staining was performed on paraffin sections. Antigen retrieval was performed with antigen unmasking solution (Vector Laboratories) or proteinase K. Endogenous peroxidase activity was quenched with 3% H2O2 for 10 min. After blocking with 5% normal serum, slides were incubated with primary antibodies in a humidified chamber overnight. After washing, slides were sequentially incubated with appropriate secondary antibodies and ABC solution according to the ABC kit (Vector Laboratories). Slides were then visualized by incubation in diaminobenzidine solution for an appropriate time duration. Nuclear staining was performed with hematoxylin. The slides were dehydrated, cleared, and mounted. The images from these slides were obtained and analyzed using the NIS Element software (Nikon Instruments) with a Nikon microscope image system (Nikon Instruments).
Zhou J., Fan Y., Tang S., Wu H., Zhong J., Huang Z., Yang C, & Chen H. (2017). Inhibition of PTEN activity aggravates cisplatin-induced acute kidney injury. Oncotarget, 8(61), 103154-103166.
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5

Immunohistochemical Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical staining was performed on paraffin sections. Antigen retrieval was performed with an antigen unmasking solution (Vector Laboratories) or proteinase K. The endogenous peroxidase activity was quenched with 3% H 2 O 2 for 10 min. After blocking with 5% normal serum, slides were incubated with primary antibodies in a humidified chamber overnight. After washing, slides were incubated with appropriate secondary antibodies and ABC solution sequentially according to the ABC kit (Vector Laboratories). Slides were then visualized by incubation in diaminobenzidine solution for an appropriate duration. Nuclear staining was performed with hematoxylin. The slides were dehydrated, cleared, and mounted. The images from these slides were obtained and analyzed by NIS Element software (Nikon Instruments) using the Nikon microscope image system (Nikon Instruments).
Zhou J., Zhong J., Lin S., Huang Z., Chen H., Tang S., Yang C, & Fan Y. (2017). Inhibition of PTEN Activity Aggravates Post Renal Fibrosis in Mice with Ischemia Reperfusion-Induced Acute Kidney Injury. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 43(5).
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