The following reagents were used: EMDM (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); FBS (Gemini Bio Products, West Sacramento, CA, USA); TRIzol (Ambion; Thermo Fisher Scientific, Inc.); MDC (Tiangen Biotech Co., Ltd., Beijing, China); WIP organization cell lysis solution (Boaoseng, Beijing, China); RNase Away (Ed lai, Beijing, China); BCA protein concentration determination kit (Blue skies, China); SDS-PA (Nanjing KeyGen Biotech. Co. Ltd., Nanjing, China); Reverse transcription kit (Lisu, Shanghai, China); Primers (Hongxun, Suzhou, China); High sensitive chemiluminescence detection kit (Qihaifutan, Shanghai, China); TRAP1 Rabbit mAb (Cell Signaling Technology, Inc., Danvers, MA, USA); Beclin1 Rabbit mAb (Cell Signaling Technology, Inc.); LC3 Rabbit mAb (Cell Signaling Technology, Inc.); Anti-rabbit IgG, HRP-linked Antibody (Isotype: Goat; Cell Signaling Technology, Inc.); Mouse anti β-actin mAb (Zhongshanjinqiao, China); Anti-β-actin Mouse Monoclonal Antibody (Isotype: Goat; Zhongshanjinqiao, China); and Protein Simple (San Jose, CA, USA).
Mouse anti β actin mab
Manufactured by Cell Signaling Technology
Sourced in United States
Mouse anti-β-actin mAb is a monoclonal antibody that recognizes the β-actin protein, a ubiquitous cytoskeletal protein found in all eukaryotic cells. This antibody can be used for the detection and quantification of β-actin in various applications such as Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Anti β actin mouse monoclonal antibody, by Cell Signaling Technology (1 mentions)
Beclin1 rabbit mab, by Cell Signaling Technology (1 mentions)
Lc3 rabbit mab, by Cell Signaling Technology (1 mentions)
Recombinant human egf, by R&D Systems (1 mentions)
Sp600125, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Cytiva (1 mentions)
Celltracker orange cmtmr, by Thermo Fisher Scientific (1 mentions)
Pd98059, by Merck Group (1 mentions)
U0126, by Merck Group (1 mentions)
Erlotinib, by Santa Cruz Biotechnology (1 mentions)
Mouse anti cx43, by Thermo Fisher Scientific (1 mentions)
U0126, by Roche (1 mentions)
Erlotinib, by Roche (1 mentions)
Cy3 conjugated goat anti rabbit igg, by Abcam (1 mentions)
Mouse anti nf κb p65 antibody, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Abcam (1 mentions)
Staurosporine, by Abcam (1 mentions)
3 protocols using mouse anti β actin mab
1
Autophagy Pathway Regulation in Cell Lines
2
Investigating EGF Signaling Pathways
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All tissue culture media were purchased from Gibco (Grand Island, NY, USA), and fetal calf serum was purchased from Hyclone Laboratories (Logan, UT, USA). Recombinant human EGF was obtained from R&D Systems (Minneapolis, MN, USA). For fluorescent imaging, CellTracker Orange CMTMR [5-(and-6)-(4-chloromethyl) benzoyl)amino)tetramethylrhodamine] was purchased from Invitrogen (Carlsbad, CA, USA).
Mouse anti-Cx43, anti-Cx32 and anti-Cx26 were purchased from Invitrogen and, mouse anti-β-actin mAb, HRP-anti-rabbit and HRP-anti-mouse IgG from Cell Signaling Technology (Billerica, CA, USA). EGFR blocking humanized antibody (Cetuximab) and humanized IgE (Omalizumab) were kind gifts from Roche (Nutley, NJ, USA).
EGFR signaling inhibition was achieved using the following inhibitors: Erlotinib (10 μM, IC50=5–20 μM), SP600125 (25 μM, IC50=10–50 μM), PD98059 (5 μM, IC50=5–10 μM) and U0126 70 μM, IC50=50–70 μM). SP600125, PD98059 and U0126 were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Erlotinib was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Mouse anti-Cx43, anti-Cx32 and anti-Cx26 were purchased from Invitrogen and, mouse anti-β-actin mAb, HRP-anti-rabbit and HRP-anti-mouse IgG from Cell Signaling Technology (Billerica, CA, USA). EGFR blocking humanized antibody (Cetuximab) and humanized IgE (Omalizumab) were kind gifts from Roche (Nutley, NJ, USA).
EGFR signaling inhibition was achieved using the following inhibitors: Erlotinib (10 μM, IC50=5–20 μM), SP600125 (25 μM, IC50=10–50 μM), PD98059 (5 μM, IC50=5–10 μM) and U0126 70 μM, IC50=50–70 μM). SP600125, PD98059 and U0126 were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Erlotinib was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
3
Signaling Pathways and Apoptosis Assay
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Rabbit anti-phosphorylated and anti-total p38MAPK mAb, rabbit anti-phosphorylated and anti-total IκB mAb, rabbit anti-phosphorylated and anti-total AKT mAb, rabbit anti-Mcl-1 mAb, rabbit anti-procaspase 3 mAb, mouse anti-active caspase-3 mAb, mouse anti-β-actin mAb, rabbit anti-IgG, and mouse anti-IgG were purchased from Cell Signaling Technology (Beverly, MA, USA). Mouse anti-His mAb was purchased from Affinity Biosciences, Inc (OH, USA). The rabbit anti-C. psittaci mAb was a gift from Professor G. Zhong (University of Texas Health Science Center at San Antonio, TX). The rabbit anti-SOD2 antibody, mouse anti-Bax antibody was purchased from ImmunoWay Biotechnology Company (Plano, TX, USA). The inhibitors SB203580, BAY117082, LY294002, staurosporine (STS), and Cy2-conjugated goat anti-rabbit IgG, DAPI, Cy3-conjugated goat anti-mouse IgG, and Cy3-conjugated goat anti-rabbit IgG and mouse anti-NF-κB p65 antibody were obtained from Abcam (Cambridge, MA, USA), recombinant human IL-8 from (Biolegend, CA, USA), anti-IL-8 mAb was obtained from GeneTex, USA.
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