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Procartaplex elisa kits

Manufactured by Thermo Fisher Scientific
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The ProcartaPlex ELISA kits are a multiplex assay platform designed for the simultaneous detection and quantification of multiple analytes in a single sample. The kits utilize magnetic beads coated with capture antibodies specific to the target analytes, allowing for the efficient and accurate measurement of protein levels in various sample types.

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Lab products found in correlation

Bio plex 200 system, by Bio-Rad (2 mentions)

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ELISAs (35 citations) ProcartaPlex kit (18 citations)

2 protocols using procartaplex elisa kits

1

Cytokine ELISA Analysis of PBMCs

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ELISA analysis for cytokine productions of IFNγ, IL-4, IL-13 and IL-5 was performed using the ProcartaPlex ELISA kits (eBioScience, Thermo-Fisher) and analyzed by Bio-Plex 200 systems (Bio-Rad). PBMCs were cultured in RPMI plus 10% FBS with 20 ng/mL PMA and 1mM ionomycin for 5–6 hours in 106 cells/mL. The media were harvested, diluted and analyzed.
Ma C.A., Stinson J.R., Zhang Y., Abbott J.K., Weinreich M.A., Hauk P.J., Reynolds P.R., Lyons J.J., Nelson C.G., Ruffo E., Dorjbal B., Glauzy S., Yamakawa N., Arjunaraja S., Voss K., Stoddard J., Niemela J., Zhang Y., Rosenzweig S.D., McElwee J.J., DiMaggio T., Matthews H.F., Jones N., Stone K.D., Palma A., Oleastro M., Prieto E., Bernasconi A.R., Dubra G., Danielian S., Zaiat J., Marti M.A., Kim B., Cooper M.A., Romberg N.D., Meffre E., Gelfand E.W., Snow A.L, & Milner J.D. (2017). Germline hypomorphic CARD11 mutations in severe atopic disease. Nature genetics, 49(8), 1192-1201.
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2

Cytokine ELISA Analysis of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISA analysis for cytokine productions of IFNγ, IL-4, IL-13 and IL-5 was performed using the ProcartaPlex ELISA kits (eBioScience, Thermo-Fisher) and analyzed by Bio-Plex 200 systems (Bio-Rad). PBMCs were cultured in RPMI plus 10% FBS with 20 ng/mL PMA and 1mM ionomycin for 5–6 hours in 106 cells/mL. The media were harvested, diluted and analyzed.
Ma C.A., Stinson J.R., Zhang Y., Abbott J.K., Weinreich M.A., Hauk P.J., Reynolds P.R., Lyons J.J., Nelson C.G., Ruffo E., Dorjbal B., Glauzy S., Yamakawa N., Arjunaraja S., Voss K., Stoddard J., Niemela J., Zhang Y., Rosenzweig S.D., McElwee J.J., DiMaggio T., Matthews H.F., Jones N., Stone K.D., Palma A., Oleastro M., Prieto E., Bernasconi A.R., Dubra G., Danielian S., Zaiat J., Marti M.A., Kim B., Cooper M.A., Romberg N.D., Meffre E., Gelfand E.W., Snow A.L, & Milner J.D. (2017). Germline hypomorphic CARD11 mutations in severe atopic disease. Nature genetics, 49(8), 1192-1201.
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