Chromosomes were analyzed in Olympus Provis AX 70 or Carl Zeiss Axioskop 20 microscopes equipped with fluorescence lamp HBO50 and appropriate filters. Images were recorded with Olympus DP30BW CCD and cooled Carl Zeiss AxioCam HRc CCD cameras and processed using AxioVision and Lucia ver. 2.0 (Laboratory Imaging) softwares.
Axiocam hrc ccd
Manufactured by Zeiss
Sourced in Germany
The AxioCam HRc CCD is a high-resolution digital camera designed for microscopy applications. It features a 12.5 megapixel CCD sensor and delivers high-quality, detailed images. The camera is compatible with various microscopy techniques and can be integrated with Zeiss imaging software.
Automatically generated - may contain errors
Lab products found in correlation
Provis ax 70, by Zeiss (1 mentions)
Axioskop 20, by Zeiss (1 mentions)
Axiovision, by Zeiss (1 mentions)
Tetramisole hydrochloride, by Merck Group (1 mentions)
Axio imager a2, by Zeiss (1 mentions)
Vectashield mounting medium, by Vector Laboratories (1 mentions)
Brn3a, by Santa Cruz Biotechnology (1 mentions)
Statistica software, by StatSoft (1 mentions)
Alexa fluor 488, by Dianova (1 mentions)
4 protocols using axiocam hrc ccd
1
Microscopy Analysis of Chromosomes
2
Visualizing elc-1 Expression in Animals
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Images of elc‐1p::elc‐1::gfp animals were taken using an AxioCam HRc CCD digital camera (Zeiss Corporation, Jena, Germany) with a Zeiss Axio Scope A1 compound microscope (Zeiss Corporation). Tetramisole hydrochloride (0.4 mm; Sigma) was used as an anesthetic.
3
Immunofluorescence Imaging of Microtubules
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Cells were fixed and incubated with antibodies as described previously (Lee and Song, 2007 (link)). Human anti-α-tubulin (1:1000, Sigma-Aldrich, #T5168) was used as a primary antibody to detect α-tubulin in the cells. DNA was detected with 0.2 μg/ml 14, 6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Coverslips were mounted with Vectashield mounting medium (Vector Laboratories, USA). Immunofluorescence images were collected at 488 nm on an Axio Imager A2 using a 40× 0.75 NA EC Plan-Neofluar objective lens and 10× ocular lens with an AxioCam Hrc CCD camera (all Carl Zeiss). The acquisition parameters and focus were controlled by the AxioVision software.
4
Quantification of Retinal Ganglion Cells
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14 days after immunization, eyes were fixed in 4% paraformaldehyde (PFA) for 1 h and then prepared as flatmounts (n = 6/group). The following steps were performed at 20°C on a thermo shaker (70 rpm). First, the flatmounts were blocked with 10% donkey serum and 0.5% Triton-X in PBS for 90 min. Then, they were incubated with the RGC marker Brn-3a (Nadal-Nicolas et al., 2009 (link)) (1:300; Santa Cruz, CA, USA) overnight, followed by a 2 h incubation of donkey anti-goat Alexa Fluor 488 (1:1000; Dianova, Hamburg, Germany). From each of the four flatmount arms, three photos were captured (central, middle, and peripheral) with an Axiocam HRc CCD camera on an Axio Imager M1 fluorescence microscope (Zeiss, Jena, Germany). Cells were counted using ImageJ software (NIH, USA). Group comparison was performed after transferring the data to Statistica software (V10.0; Statsoft, Tulsa, OK, USA).
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