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SC-888 is a laboratory centrifuge designed for general-purpose applications. It provides high-speed separation of samples in a variety of tube sizes. The centrifuge features a durable, well-balanced rotor and a digital display for speed and time control.

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Lab products found in correlation

Esgro, by Merck Group (1 mentions) F1804, by Merck Group (1 mentions) Ab55603, by Abcam (1 mentions) Sc 585x, by Santa Cruz Biotechnology (1 mentions) Ab4729, by Abcam (1 mentions) Ab8895, by Abcam (1 mentions) Ab21635, by Abcam (1 mentions)

2 protocols using sc 888

1

Hoxb1 ChIP-seq in ES cell differentiation

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ES cells were cultured in feeder-free conditions using N2B27 + 2i media supplemented with 2000U mL−1 of ESGRO (Millipore) on a gelatinized plate. KH2 ES cells72 (link) with epitope-tagged Hoxb1 (3XFLAG-MYC) were used for Hoxb1 ChIP using anti-flag antibody (F1804-Sigma). Unmodified KH2 cell lines were used for ChIP experiments for Pbx (SC-888; Santa Cruz), Meis (SC-25412; Santa Cruz), Prep1 (ab55603; Abcam), Prep2 (sc-292315X; Santa Cruz) and EP300 (Sc-585X; Santa Cruz). Cells were differentiated to neuroectoderm in differentiation media containing DMEM + 10% (vol/vol) Serum + NEAA + 3 µM RA for a requisite length of time. Cells were harvested at 80–90% confluency.
Parker H.J., De Kumar B., Green S.A., Prummel K.D., Hess C., Kaufman C.K., Mosimann C., Wiedemann L.M., Bronner M.E, & Krumlauf R. (2019). A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates. Nature Communications, 10, 1189.
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2

Transcription Factor ChIP-seq and ATAC-seq

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Anti-Flag antibody was used to pull down 3XFLAG tagged-HOXB1 for ChIP-seq experiments. We used the Upstate protocol for ChIP-seq experiments as described in Smith et al [38] (link). All other ChIP-seq experiments were done using specific antibodies against the transcription factors (Anti-PBX: Santacruz; SC-888, Anti-MEIS: Santacruz; SC-25412, Anti-REST: Abcam; ab21635) and histone proteins (Anti-H3K27Ac: Abcam; ab4729, Anti-H3K4me3: Abcam; ab8895). Expression analysis of the genes at various time points (0, 4, 6, 12, 24 and 36 hour) after RA differentiation of ES cells were analysed using RNA-seq [39] (link). Buenrostro and colleagues protocol was used for the chromatin accessibility (ATAC-seq) experiment using approximately 50,000 feeder-free uninduced and differentiated ES cells [40] (link)
Singh N.P., Kumar B.D., Paulson A., Parrish M.E., Scott C., Zhang Y., Florens L., & Krumlauf R. (2020). Genome-wide binding analyses of HOXB1 revealed a novel DNA binding motif associated with gene repression.
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